FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266259
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGGCACAGAACTCAGCTTCAGGTAAA69340.5475972924970326No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG50180.3962854360758739No Hit
GTGTATTACTGTGCGACCATGGGATATTGTAGTGGTGGTAGCTGCCACAA42170.33302823513988844No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA38150.301281175494113No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34320.27103459876691893No Hit
GTCTCTGGTGGCTCCATCAGCGGTAATAGTTACTACTGGACCTGGATCCG30940.24434179737320721No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30620.24181466824717535No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC29010.22910004983182747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27400.21638543141647956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27380.21622748584610257No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT26050.2057241054160326No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGGCACAGAACTCAGCTTCAGGTA25360.20027498323802634No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24510.19356229649700418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23060.18211124264467224No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATC22990.18155843314835277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22880.1806897325112793No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21600.1705812160071518No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGGTCCAGTAGTAACTATTACCGCTGATGGAG21320.1683699780218739No Hit
GGCTAACAGTTTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT21100.16663257674772697No Hit
GTATTACTGTGCGACCATGGGATATTGTAGTGGTGGTAGCTGCCACAACTGGTTCGACCCCTGGGGCCAGGGAAC20840.16457928433282606No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT20550.1622890735623597No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20220.15968297165113932No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19960.1576296792362384No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATTAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA19950.1575507064510499No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19470.1537600127620021No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18990.14996931907295427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18960.14973240071738877No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18930.1494954823618233No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18570.14665246209503743No Hit
GTCCATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT18270.14428327853938255No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC18250.14412533296900557No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC17500.13820237407986832No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17420.13757059179836037No Hit
GGATTGGGAGTGTCCATTATAGTGGGAGCACCTACTACAACCCGTCCCTC17080.13488551710195149No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA16830.1329111974722391No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16760.13235838797591962No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT16740.13220044240554263No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16590.13101585062771517No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT16490.1302261227758302No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16420.12967331327951073No Hit
CCATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA16330.12896255821281427No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16320.1288835854276258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16190.12785693922017533No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC15790.12469802781263548No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15760.12446110945707001No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15650.12359240881999653No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15620.12335549046443106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15590.12311857210886556No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG15570.12296062653848858No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15460.1220919259014151No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA15300.12082836133839917No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15270.12059144298283367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15050.11885404170868677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15030.11869609613830978No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT14940.11798534107161332No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCC14820.11703766764935136No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14790.11680074929378587No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC14600.11530026637520444No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGTGGCAGCTACCACCACTA14510.11458951130850797No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14500.11451053852331947No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG14450.114115674597377No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG14380.11356286510105752No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14300.11293108281954956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14280.11277313724917257No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14160.11182546382691061No Hit
GGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGG13740.10850860684899377No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC13460.10629736886371588No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT13370.1055866137970194No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA13370.1055866137970194No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG13320.10519174987107693No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGT13310.10511277708588843No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA13090.10337537581174151No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12970.10242770238947956No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC12950.10226975681910257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGAAA12940.10219078403391407No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC12890.10179592010797159No Hit
GGACTGGTGAAGCCTTCGGAGACCCTGTCCGTCACCTGCGCTGTCTCTGGTGGCTCCATCAGCGGTAATAGTTA12700.10029543718939017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)