FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47550.35577097178223677No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACG45130.33766443652013345No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTGTGCAGC43560.3259176347178598No Hit
CCATAGTAGAGCAGGCAGTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCCTTGCCC42690.31940826047074No Hit
GTCCAGGAGTGTTTGTAAGTTGTACCATAAATCAGTGCCCTGGGTGCTTG39380.2946427101742268No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACGAGTACCACCATAGTAGAGCAGGCAGTAATAATCAG38320.2867117484478509No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37800.28282108797830807No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36920.27623689333754325No Hit
GATTATTACTGCCTGCTCTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG36270.2713735677506146No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA33810.2529677509139311No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT33710.25221954697748056No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33530.25087277989186957No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG33110.24773032335877726No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30300.22670579274451677No Hit
GTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTG30200.2259575888080662No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30110.22528420526526072No Hit
GTGATAACCATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGG29800.22296477306226406No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC28460.21293884031382665No Hit
CTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCC28390.21241509755831126No Hit
GATTTATGGTACAACTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGC27990.20942228181250908No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27210.2035862911081948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26230.19625389253097938No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25270.1890711347410541No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25150.18817329001731345No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24740.18510565387786618No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT24360.1822624789193541No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23880.17867110002439146No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTAAGTTGTACCATAAAT23850.1784466388434563No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAA23450.1754538230976541No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23240.17388259483110793No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22860.17103941987259583No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22630.16931855081875957No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22200.1661012738920222No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC21610.16168687066696394No Hit
GTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTAAGTTGTAC21380.15996600161312768No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21370.15989118121948262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20760.15532713720713426No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20670.15465375366432876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20470.15315734579142767No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20450.15300770500413757No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20430.15285806421684744No Hit
CCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTTATCACTGGTGACTGCT19710.1474709958744035No Hit
GCCTGAGGACGAGGCTGATTATTACTGCCTGCTCTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAG19310.14447818012860128No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19160.14335587422392546No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC18640.13946521375438262No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18360.13737024273232104No Hit
GCACTGGAGCAGTCACCAGTGATAACCATCCAAACTGGTTCCAGCAGAAA18240.13647239800858038No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACGAGTACCACCATAGTAGAGCAGGCAGTAATAATCAGC17910.13400332501829357No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17880.1337788638373584No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTC17580.13153425202800675No Hit
ACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA16970.12697020801565842No Hit
CACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAACCATCCAAACT16970.12697020801565842No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16800.12569826132369247No Hit
CCTGACAGTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCG16730.12517451856817707No Hit
GTTTGTAAGTTGTACCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTTAT16480.12330400872705072No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16040.1200119114066683No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15940.11926370747021775No Hit
CTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAACCATCCA15810.11829104235283201No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15550.1163457121180606No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15320.11462484306422434No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15220.1138766391277738No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15030.11245505164851773No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGATTATTACTG14920.11163202731842212No Hit
CTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGG14810.11080900298832654No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14430.10796582802981443No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14350.10736726488065398No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14300.1069931629124287No Hit
GCCTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAGGAGTG14090.10542193464588255No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14030.10497301228401222No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13920.10414998795391663No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13760.10295286165559574No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13480.1008578906335342No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13430.10048378866530892No Hit
GGTTATCACTGGTGACTGCTCCAGTGCTGGAAGCACAGGTGAGAGTGACT13390.10018450709072871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)