FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336534
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTGTGCAGC44270.33122988266665865No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43010.3218025130673817No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACG41490.31042981323333335No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38570.2885822582889773No Hit
GTCCAGGAGTGTTTGTAAGTTGTACCATAAATCAGTGCCCTGGGTGCTTG38470.28783405435252674No Hit
CCATAGTAGAGCAGGCAGTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCCTTGCCC38070.28484123860672456No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35710.26718362570649157No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACGAGTACCACCATAGTAGAGCAGGCAGTAATAATCAG35300.2641159895670443No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA35100.2626195816941432No Hit
GATTATTACTGCCTGCTCTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG34910.26119799421488715No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG32160.24062238596249705No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT30730.22992306967125417No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC30470.22797773943648275No Hit
GTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGCCCTGACACTGTCAGGTG30350.22707989471274204No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30260.22640651116993654No Hit
GTGATAACCATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGG30020.22461082172245522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29270.2189992921990761No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28350.21211581598373105No Hit
GATTTATGGTACAACTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGGGGCAAGGCTGC27840.20829997590783325No Hit
CTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCC27620.20665392724764203No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27540.2060553640984816No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27280.20411003386371016No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26310.19685245568013982No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26260.19647835371191455No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25160.18824811041095849No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25070.187574726868153No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23350.17470561916120353No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTAAGTTGTACCATAAAT22240.1664005554666024No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21990.16453004562547605No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAA21970.16438040483818592No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21670.16213579302883427No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21640.1619113318478991No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21550.1612379483050936No Hit
GTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTAAGTTGTAC21460.16056456476228811No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21230.15884369570845186No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC21200.1586192345275167No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21180.1584695937402266No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20670.15465375366432876No Hit
GCCTGAGGACGAGGCTGATTATTACTGCCTGCTCTACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAG20170.15091273398207602No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC19840.1484436609917892No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19760.14784509784262878No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC19060.14260767028747492No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18980.14200910713831447No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18710.13998895650989798No Hit
CCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTTATCACTGGTGACTGCT18570.13894147099886722No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18100.13542491249754965No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17930.1341529658055837No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17240.1289903586440749No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17110.12801769352668918No Hit
GCACTGGAGCAGTCACCAGTGATAACCATCCAAACTGGTTCCAGCAGAAA16890.12637164486649796No Hit
GTTTGTAAGTTGTACCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTTAT16820.12584790211098257No Hit
ACTATGGTGGTACTCGTGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA16620.12435149423808149No Hit
CACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAACCATCCAAACT16560.12390257187621116No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16520.12360329030163093No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCGGGCAGGGGTC16270.12173278046050456No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16120.12061047455582874No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15590.11664499369264082No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15270.11425074109599904No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACACGAGTACCACCATAGTAGAGCAGGCAGTAATAATCAGC14970.1120061292866474No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14780.11058454180739137No Hit
CTCTCACCTGTGCTTCCAGCACTGGAGCAGTCACCAGTGATAACCATCCA14700.10998597865823091No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14690.10991115826458588No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14640.10953705629636058No Hit
CCTGACAGTGTCAGGGCAGCCTTGCCCCCAAGGAGGGAACCTGAGAACCG14330.10721762409336387No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14260.10669388133784849No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14150.10587085700775288No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14140.10579603661410783No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13790.1031773228365309No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13680.1023542985064353No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13650.10212983732550014No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGATTATTACTG13640.10205501693185508No Hit
CTTACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGTTCCCTCCTTGGG13570.1015312741763397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)