FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327766
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC71410.5378206702084554No Hit
GCCTTGGGCTGACTGAGGACGGTCAGTTTGGTGCCTCCGCCGAAAAAAGT66260.4990337152781439No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTACTATCTGCCTTCCAG47280.35608684060293755No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43780.3297267741454443No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGTTTGGTGCCTC43560.3280698556824019No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40550.30540019852895767No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT39550.29786875096967386No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36970.2784376162667217No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATATCCTG33010.24861308393195786No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32220.24266324036012368No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTT31630.23821968630014626No Hit
GACTGAGGACGGTCAGTTTGGTGCCTCCGCCGAAAAAAGTGCCACTCTGTGAATATGAACTGCAATAATAATGAG31530.23746654154421787No Hit
TGGTAAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATCGGAAACCCCTGAGGGCCGATCACTGACGT31490.23716528364184655No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31390.23641213888591817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31370.2362615099347325No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29920.22534091097377099No Hit
GGGCTACTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC29020.21856260817041556No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28840.2172069476097445No Hit
CTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28640.21570065809788774No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTACTATCTGCCTTCCAGGCCACTGTCACGGCTC28530.2148721988663665No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28480.2144956264884023No Hit
GTGTTGGTACCAAGAGACAAGATTATATGTAAGAAGACTGTCTCCAGTGC28380.21374248173247393No Hit
GTCCAGAGATGGTAAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATCGGAAACCCCTGAGGGCCGAT28200.21238682117180285No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27150.20447880123455486No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27130.20432817228336922No Hit
CCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG26720.20124027878406286No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26700.20108964983287717No Hit
TAGTAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG26680.2009390208816915No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25530.19227785618851514No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGG25520.1922025417129223No Hit
GTTTGGTGCCTCCGCCGAAAAAAGTGCCACTCTGTGAATATGAACTGCAATAATAATGAGCCTCGTCGGCAGC24880.18738241527498067No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24640.18557486786075258No Hit
CCCCTGAGGGCCGATCACTGACGTCATAAATCAGGAGTTTGGGGACTTTGCCTGGGTGGTGTTGGTACCAAGAGA24570.1850476665316027No Hit
GGATATGGTGATCGCCTGTCCAGGAGACCCAGACACGGAGGCAGGTTGAGTCAGGGCAGACTGGGCCCAGGACC23170.1745036399486054No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22980.1730726649123415No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22820.1718676333028561No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22430.1689303687547354No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21710.16350772651205106No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAC21630.16290521070730837No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21510.16200143700019431No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21460.1616248646222301No Hit
GTGAATATGAACTGCAATAATAATGAGCCTCGTCGGCAGCCTGGAGTCCAGAGATGGTAAGGGAGGCCGTGTCGC20580.15499719077006038No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20310.15296369992905376No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20130.15160803936838269No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGTCAGTTTGGTGCCTCCGCCGAAAAAAGTGCCACT19750.14874608929585484No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC19420.14626071160129117No Hit
TTATTATTGCAGTTCATATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCTCAGTCAGCC19230.14482973656502726No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18970.1428715601996135No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG18910.14241967334605646No Hit
GTGCAGGATATGGTGATCGCCTGTCCAGGAGACCCAGACACGGAGGCAGG17730.13353256522610157No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17730.13353256522610157No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGT17490.13172501781187348No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC17400.13104718753153793No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17370.13082124410475943No Hit
GCACCAAACTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16910.12735677822748886No Hit
ACTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16900.12728146375189606No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGTAGCC16890.12720614927630322No Hit
ATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACC16730.1260011176668178No Hit
GCAATAATAATGAGCCTCGTCGGCAGCCTGGAGTCCAGAGATGGTAAGGG16470.12404294130140402No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16370.12328979654547562No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16290.12268728074073293No Hit
TATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCT16230.1222353938871759No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16080.12110567675328333No Hit
CTCCACTCCCGCCTTGACGGGGCTACTATCTGCCTTCCAGGCCACTGTCA16020.1206537898997263No Hit
CATTATTATTGCAGTTCATATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCTCAGTCAG15810.11907218591227671No Hit
GCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15580.11733995297364143No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15350.11560772003500618No Hit
ACCATATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAAGTC15130.11395080157196374No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT14970.11274576996247833No Hit
CCTGAGGGCCGATCACTGACGTCATAAATCAGGAGTTTGGGGACTTTGCC14870.11199262520654996No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAGGACGGT14840.11176668177977143No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTGAG14770.11123948045062156No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14700.1107122791214717No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14510.10928130408520778No Hit
CATATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCT14480.10905536065842927No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14310.10777501457335102No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGGTACAGCTGC14280.10754907114657253No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATAT14130.10641935401267993No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA13810.10400929079370913No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13700.10318083156218791No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13610.10250300128185237No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13570.10220174337948103No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13520.10182517100151683No Hit
GTACCAACACCACCCAGGCAAAGTCCCCAAACTCCTGATTTATGACGTCA13520.10182517100151683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)