FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327766
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC68650.5170338749448321No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATATCCTG54980.41407898680942273No Hit
GTACCAACACCACCCAGGCAAAGTCCCCAAACTCCTGATTTATGACGTCA50370.3793590135611245No Hit
CCCTTACCATCTCTGGACTCCAGGCTGCCGACGAGGCTCATTATTATTGC42530.32031246469633956No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40090.3019357326516871No Hit
ATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACC38590.29063856131276145No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37960.2858937493504127No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36770.2769313267548649No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAC36310.2734668608775944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36170.27241245821929466No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35210.26518226856238225No Hit
TCTCTGGACTCCAGGCTGCCGACGAGGCTCATTATTATTGCAGTTCATAT34390.25900648156376954No Hit
ACCATATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAAGTC33530.2525294366627855No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTACTATCTGCCTTCCAG31200.23498116384965423No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29720.22383462146191424No Hit
TGGTAAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATCGGAAACCCCTGAGGGCCGATCACTGACGT29340.22097267138938637No Hit
GTGTTGGTACCAAGAGACAAGATTATATGTAAGAAGACTGTCTCCAGTGC29110.2192404384507511No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28170.21216087774502435No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTT27800.20937424214808936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27710.2086964118677538No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAAC27360.20606040522200447No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27350.20598509074641164No Hit
GTCCAGAGATGGTAAGGGAGGCCGTGTCGCCAGACTTGGAGCCAGAGAAGCGATCGGAAACCCCTGAGGGCCGAT26460.19928210241864905No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25250.19016905087191568No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25130.18926527716480163No Hit
TAGTAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG24120.18165851512992498No Hit
CATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAAGTCCCCAAA24020.18090537037399662No Hit
GACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATAT23970.18052879799603244No Hit
CCATATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAAGTCC23680.17834467820384015No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23650.1781187347770616No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23520.17713964659435474No Hit
TCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23320.17563335708249797No Hit
CCCCTGAGGGCCGATCACTGACGTCATAAATCAGGAGTTTGGGGACTTTGCCTGGGTGGTGTTGGTACCAAGAGA22940.17277140700997012No Hit
CTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22900.17247014910759878No Hit
GTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAA22600.17021071483981365No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22520.16960819903507093No Hit
GGATATGGTGATCGCCTGTCCAGGAGACCCAGACACGGAGGCAGGTTGAGTCAGGGCAGACTGGGCCCAGGACC21690.1633570975608654No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21510.16200143700019431No Hit
CCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21270.1601938895859662No Hit
AGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCG21110.1589888579764808No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20970.15793445531818107No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20730.15612690790395295No Hit
GCCCTCAGGGGTTTCCGATCGCTTCTCTGGCTCCAAGTCTGGCGACACGG20670.15567502105039593No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20260.15258712755108958No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTACTATCTGCCTTCCAGGCCACTGTCACGGCTC19760.14882140377144767No Hit
GCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATATCCTGCACTGGAGACAGTCTT19360.14580882474773416No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19130.1440765918090989No Hit
CTCCTGGACAGGCGATCACCATATCCTGCACTGGAGACAGTCTTCTTACA18750.14121464173657106No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC18250.13744891795692915No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17800.13405976655525145No Hit
TTATTATTGCAGTTCATATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCTCAGTCAGCC17700.1333066217993231No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCT17380.13089655858035226No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17350.13067061515357375No Hit
GGGCTACTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17190.12946558354408833No Hit
GTCCCCAAACTCCTGATTTATGACGTCAGTGATCGGCCCTCAGGGGTTTCCGATCGCTTCTCTGGCTCCAAGT17010.12810992298341725No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT16890.12720614927630322No Hit
GTGCAGGATATGGTGATCGCCTGTCCAGGAGACCCAGACACGGAGGCAGG16860.12698020584952469No Hit
GGCGATCACCATATCCTGCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGG16650.12539860186207508No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16440.1238169978746255No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16370.12328979654547562No Hit
CCTCAGGGGTTTCCGATCGCTTCTCTGGCTCCAAGTCTGGCGACACGGCCTCCCTTACCATCTCTGGACTCCAGG16170.12178350703361887No Hit
ACCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCACCATATCCTGCA16090.12118099122887618No Hit
CTCCTGATTTATGACGTCAGTGATCGGCCCTCAGGGGTTTCCGATCGCTT15710.11831904115634834No Hit
TATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCT15560.11718932402245577No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGTAGCC15530.11696338059567725No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGGTACAGCTGC15090.11364954366959237No Hit
GCACCAAACTGACCGTCCTCAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15080.11357422919399955No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT14670.11048633569469318No Hit
CATTATTATTGCAGTTCATATTCACAGAGTGGCACTTTTTTCGGCGGAGGCACCAAACTGACCGTCCTCAGTCAG14650.11033570674350753No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTACCTTTTGT14570.10973319093876481No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCC14360.10815158695131522No Hit
GTGGAAGTCCCACAAAAGGTACAGCTGCCAGGTCACGCATGAAGGGAGCA14310.10777501457335102No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14260.10739844219538684No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14250.10732312771979399No Hit
CTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC13980.10528963687878737No Hit
GCACTGGAGACAGTCTTCTTACATATAATCTTGTCTCTTGGTACCAACACCACCCAGGCAAAGTCCCCAAACTC13810.10400929079370913No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT13790.10385866184252346No Hit
CCTGAGGGCCGATCACTGACGTCATAAATCAGGAGTTTGGGGACTTTGCC13630.10265363023303806No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC13610.10250300128185237No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13500.10167454205033115No Hit
GTTCATGTGAAAGCCCAAGAAGATGAAAGGATTGTTCTTGTTGACAACAA13410.10099671176999561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG300.0027758866.19775470
GGTATCA10400.046.4276581
GTGGTAT2300.044.35221
GTATCAA27250.036.8109661
TGGTATC2750.035.8513262
TATCAAC29000.034.114182
TCAACGC30750.032.386524
ATCAACG30900.032.22933
CAACGCA31500.031.615415
AACGCAG31950.031.2765056
AATGGTA10550.030.11840870
ACGCAGA33800.029.4640667
GTGTATA1052.6844646E-829.1457331
CGAAATT605.044337E-429.10739548
GTAGTTT1155.385149E-428.78163170
CGCAGAG34700.028.6019158
GTGCTAG2800.027.9313261
AGAGTAC35750.027.66365611
CAGAGTA35900.027.35873610
TAGCTCG500.00831918927.191415
Produced by FastQC (version 0.11.5)