FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563813
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG144260.9224888141996518No Hit
GACTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT120840.7727266623311099No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC81160.5189878841012321No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGGCATCCCATGTTGCA74210.4745452301521985No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC65310.41763305459156563No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG64400.41181394450615255No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT64360.4115581594474531No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGGCATCCCATGTTGCACAGTAATAGT60250.38527624466608223No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC55940.3577154045912139No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT55330.3538146824460469No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG54410.34793162609595907No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51750.330921919692444No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT51510.32938720934024723No Hit
GGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT46390.2966467218267146No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC44190.2825785435982435No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43580.27867782145307657No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC43260.27663154098348075No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42080.2690858817518463No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG41950.268254580311073No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41040.26243547022566No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC40410.25840685555114323No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT39330.25150065896625745No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38980.24926253970263706No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38780.2479836144091397No Hit
CTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAG38380.24542576382214498No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC37510.23986243879543143No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA37340.23877535229595864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36290.23206099450509748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT34730.222085377215818No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG34550.22093434445167037No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34490.22055066686362118No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA33340.2131968464260113No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAG32680.20897639295747No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA31660.20245387396063338No Hit
CCCCTGAGGGCCGCTGACTATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA30880.19746606531599367No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30210.19318166558277747No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC29150.18640336152724143No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28840.18442102732232052No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA28680.1833978870875226No Hit
GGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA28610.18295026323479854No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28270.180776090235853No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT28260.18071214397117813No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACTGAGGTCAGCCTCAGGGAGA27810.17783456206080908No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC27490.17578828159121326No Hit
GTCTGAGGATGAGGCTGACTATTACTGTGCAACATGGGATGCCAGCCTGA27470.17566038906186354No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC27290.1745093562977159No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC27280.17444541003304104No Hit
CTCTTGTTCTGGAAGCAGTTCAAACATCGGAAGTAACGCTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC25880.16549293297855946No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25010.1599296079518459No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT24880.1590983065110726No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24560.15705202604147683No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGG24330.15558126195395486No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24020.15359892774903391No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTGCAA23470.15008188319191618No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23390.1495703130745172No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG23350.14931452801581774No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23030.14726824754622198No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC23010.14714035501687223No Hit
GCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA22800.1457974834587No Hit
GTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22720.14528591334130103No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22270.14240833143093196No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGG22260.14234438516625708No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22220.1420886001075576No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21880.1399144271086121No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC21870.13985048084393722No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21750.13908312566783881No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGATGATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG21430.137036845198243No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21030.13447899461124826No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20650.13204903655360328No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20610.1317932514949038No Hit
GTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20510.13115378884815512No Hit
GTTCAAACATCGGAAGTAACGCTGTAAACTGGTACCAGCAGCTCCCAGGA20300.12981091728998287No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGCGTTACTT20290.12974697102530802No Hit
GTGTATTACTGTGCGAGAGGAGGGATACAGTTTTGGGACTGGGGCCAGGG19840.12686938911493892No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG19780.12648571152688973No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT19730.12616598020351538No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19660.12571835635079132No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19650.12565441008611644No Hit
ACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC19470.12450337732196881No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19390.12399180720456986No Hit
ATGTTGCACAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATG18930.12105027902952592No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18910.12092238650017617No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18900.1208584402355013No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG18680.11945162241265421No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18650.11925978361862959No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18610.11900399855993012No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18390.11759718073708302No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18370.11746928820773328No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17910.11452776003268933No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT17840.11408013617996526No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG17760.11356856606256631No Hit
GGATGAGGCTGACTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGAC17670.11299304968049248No Hit
CTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC17460.11165017812232024No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17360.11101071547557158No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17180.10985968271142393No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17130.1095399513880496No Hit
GCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGGCATCCCATGTTGCA17060.10909232753532551No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC17060.10909232753532551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17000.1087086499472763No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG16940.10832497235922708No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG16790.10736577838910406No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16630.10634263815430618No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG16470.10531949791950827No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG16390.10480792780210933No Hit
GTCAGGGACCCCTGAGGGCCGCTGACTATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA16370.1046800352727596No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC16260.10397662636133605No Hit
GCCCCCAAACTCCTCATCTATGATGATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT16200.10359294877328683No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAG16120.10308137865588787No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC16120.10308137865588787No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC16100.10295348612653814No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTTCAAACATCGGAAGTAACGCT16060.10269770106783867No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15960.10205823842108999No Hit
GACTATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG15780.10090720565694236No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15670.10020379674551881No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15640.1000119579514942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAT8150.061.1449370
AGGGGGG4300.057.15171470
ACGCGTA3500.053.45271727
CGTATGC3500.053.2755841
TCGTATG3500.053.16500540
GCGTAGT3550.052.74038329
GACGCGT3600.051.9496326
ATCTCGT3400.051.45625337
CGCGTAG3650.051.2724428
TCTCGTA3350.051.2697738
GGTATCA13650.049.5584371
CGTAGTA3850.048.6650430
ACGAGAC3850.048.5436522
ATGCCGT3800.048.39365444
GTAATCT3550.048.15715834
GCCGTCT3850.047.9342146
CGAGACG3900.047.9228323
CCCACGA3850.047.65494519
AATCTCG3600.047.5909136
CCGTCTT3900.047.3988847
Produced by FastQC (version 0.11.5)