FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563813
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT118900.7603210869841854No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG90930.5814633846885785No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC78320.5008271449335694No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC73520.47013293788963256No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67090.42901548970369224No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGGCATCCCATGTTGCA63880.4084887387430594No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60450.38655516995957956No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT56520.36142428794235626No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGGCATCCCATGTTGCACAGTAATAGT53150.3398743967469256No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC50070.32017894722706614No Hit
GGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT49210.3146795684650275No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT48920.3128251267894563No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48730.3116101477606338No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48410.309563867291038No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48400.30949992102636315No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47200.3018263692653789No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT45820.2930017847402471No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG44370.28372957636239116No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC43790.28002069301124877No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41710.26671986995887614No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC39030.2495822710260114No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38270.24472235491072142No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC37300.23851956723725917No Hit
CTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAG36620.23417122123936812No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35970.2300147140355017No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAG35900.22956709018277763No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35440.22662556200773368No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34810.22259694733321694No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34630.2214459145690693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34550.22093434445167037No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33260.21268527630861236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32850.21006347945694273No Hit
CTCTTGTTCTGGAAGCAGTTCAAACATCGGAAGTAACGCTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC31030.1984252592861167No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT30930.197785796639368No Hit
GGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30460.1947803221996492No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30340.19401296702355078No Hit
CCCCTGAGGGCCGCTGACTATCATCATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA29970.19164695523058065No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29860.19094354631915708No Hit
GTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA29750.19024013740773352No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT29730.1901122448783838No Hit
GTCTGAGGATGAGGCTGACTATTACTGTGCAACATGGGATGCCAGCCTGA29000.1854441675571184No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28440.1818631767353258No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC27570.17629985170861223No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT26780.17124809679929762No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26570.16990522524112536No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC26310.1682426223595788No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC26090.1668358045367317No Hit
GCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA25980.16613239562530815No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25810.16504530912583537No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25320.16191194215676682No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACTGAGGTCAGCCTCAGGGAGA24980.15973776915782129No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24360.15577310074797945No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG24100.15411049786643288No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC24080.15398260533708313No Hit
GTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG22900.1464369461054487No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22730.1453498596059759No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22660.1449022357532518No Hit
GTTCAAACATCGGAAGTAACGCTGTAAACTGGTACCAGCAGCTCCCAGGA22580.14439066563585287No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC22580.14439066563585287No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTGCAA22340.14285595528365605No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22160.1417049225195084No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22030.1408736210787351No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21750.13908312566783881No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGATGATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG21590.1380599854330409No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGG21050.134606887140598No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20950.13396742449384932No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG20940.13390347822917448No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20730.13256060667100222No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20670.132176929082953No Hit
GTGTATTACTGTGCGAGAGGAGGGATACAGTTTTGGGACTGGGGCCAGGG20600.13172930523022894No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGTTCAAACATCGGAAGTAACGCT20570.13153746643620431No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGCGTTACTT20560.13147352017152947No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAGCCCATTCAGGCTGG20130.1287238307905101No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA19460.12443943105729394No Hit
CTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC18880.12073054770615156No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC18850.12053870891212697No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG18660.11932372988330446No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC18650.11925978361862959No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG18570.11874821350123063No Hit
GCCCCCAAACTCCTCATCTATGATGATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT18460.1180448045898071No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18460.1180448045898071No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18400.11766112700175788No Hit
GGATGAGGCTGACTATTACTGTGCAACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGAC18390.11759718073708302No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18140.1159985241202113No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18120.11587063159086157No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18110.1158066853261867No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18100.11574273906151183No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18060.11548695400281235No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAGGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18040.11535906147346262No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG17950.11478354509138881No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17950.11478354509138881No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17870.11427197497398986No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC17810.11388829738594064No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG17740.11344067353321657No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT17520.11203385571036946No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17240.11024336029947315No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17240.11024336029947315No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAG17220.11011546777012342No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17020.10883654247662604No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16870.10787734850650302No Hit
ACATGGGATGCCAGCCTGAATGGGCTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCC16780.1073018321244292No Hit
GGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCT16620.10627869188963131No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16540.10576712177223235No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGTTCAAACATCGGAAGTAACGCTGTAAA16370.1046800352727596No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16260.10397662636133605No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16060.10269770106783867No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16050.10263375480316381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTTA200.00598364554.53007564
TACAGAT7700.049.21741570
GGTATCA15050.045.551191
GTGGTAT3900.045.2505231
TGGTATC3850.044.943742
TTGACTA804.1836756E-1142.4033623
GTATCAA42600.041.9045371
GCCAGAG31800.041.0489170
AATGGTA3800.040.6307570
CTTGACT1200.036.7554632
TATCAAC50550.035.16982
ATCAACG52550.034.148633
CGTAAAC400.002747912734.10380632
GTCGTAA400.002768338434.0523330
TCAACGC52800.033.8584444
CAACGCA53650.033.3230745
AACGCAG54750.032.588486
TCAGCAT11100.032.24504570
CGCAGAG57050.031.274668
GCTTGTG1209.458745E-1131.1097351
Produced by FastQC (version 0.11.5)