FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407595
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC63460.4508399077859754No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATAGTGGTTA52200.37084530706630814No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49530.3518767827393533No Hit
AGCCAGGACAGGCCCCTGTTCTTATCATCTATGCTAAGAACAATCGGCCC47890.3402257041265421No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46900.3331924310614914No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44440.3157158131422746No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC44410.31550268365545486No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC43480.30889566956404363No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAAAACTTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT39930.2836753469570438No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36220.2573183337536721No Hit
CTATTACTGTAACTCCCGGGACAACAGTAATAACCACTATGTGGTTTTCG35640.2531978303418242No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35610.25298470085500446No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCCGCTGTGTCTGTGGCCTTGGGA35270.2505692333377143No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34810.24730124787314534No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCCGCT33980.24140466540446648No Hit
GAACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAAAACTTT32230.22897211200664963No Hit
GACTATTACTGTAACTCCCGGGACAACAGTAATAACCACTATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTC31760.2256330833798074No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30900.21952337142430883No Hit
GGTTATTACTGTTGTCCCGGGAGTTACAGTAATAGTCAGCCTCATCTTCC30250.2149055658765483No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA30040.21341365946881025No Hit
ACTCAGGACCCCGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC30030.21334261630653706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29940.21270322784607787No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29900.21241905519698492No Hit
CCCCTGTTCTTATCATCTATGCTAAGAACAATCGGCCCCCAGGGATCCCA29770.21149549408743282No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29540.2098615013551483No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29520.20971941503060182No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29260.20787229281149763No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAAGTTTTATGCAAGCTGGTA28990.20595412743012018No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28990.20595412743012018No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG28980.20588308426784693No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT28980.20588308426784693No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA28220.20048380393508075No Hit
GTAATAACCACTATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTA27900.1982104227423371No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26140.18570682618224704No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26090.18535161037088083No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25630.18208362490631183No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25590.18179945225721889No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25420.1805917184985738No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24970.17739477619627805No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24930.17711060354718508No Hit
GTATTTCCAGAGTGGGAGCCAGAGAATCGGTCTGGGATCCCTGGGGGCCG24210.1719954958635119No Hit
CTGGAAATACAGCTTCCTTGACCATCACTGGGGCTCAGGCGGAAGATGAG23820.16922481253485555No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23500.16695143134211188No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23380.16609891339483304No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23190.16474909331164148No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22470.15963398562796827No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20650.14670413009423877No Hit
GTTCTTATCATCTATGCTAAGAACAATCGGCCCCCAGGGATCCCAGACCG20230.1437203172787627No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTACTGTAACTCCCGG19950.14173110873511202No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19950.14173110873511202No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19750.14031024548964724No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCCGCTGTGTCTGTGG19620.13938668438009513No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG19610.1393156412178219No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC19500.13853416643281627No Hit
CTTCTGAGCTGACTCAGGACCCCGCTGTGTCTGTGGCCTTGGGACAGACA19040.13526618096824725No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18590.1320692386659515No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC18470.13121672071867263No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18240.12958272798638812No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18030.12809082157865012No Hit
GTCAGGATCACATGCCAAGGAGACAGCCTCAGAAAGTTTTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCC17980.1277356057672839No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACAGTGACTTGTCGGCGTTGCCCCTGGA17490.12425449081589521No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17390.1235440591931628No Hit
GGACAGGCCCCTGTTCTTATCATCTATGCTAAGAACAATCGGCCCCCAGGGATCCCAGACCGATTCTCTGGCTCC17280.12276258440815718No Hit
GCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTATTTCCAGAGTGGGAGCCAGAGAATCGGT17240.12247841175906421No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17190.12212319594769802No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17140.12176798013633183No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17120.12162589381178536No Hit
GTTTTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTTCTTATCATCTATGCTAAGAACAATCGGCC17060.12119963483814593No Hit
GCTTGGTCCCTCCGCCGAAAACCACATAGTGGTTATTACTGTTGTCCCGGGAGTTACAGTAATAGTCAGCCTCAT17000.12077337586450648No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16870.11984981475495436No Hit
GCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAA16480.11707913142629804No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16340.1160845271544727No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACT16000.11366905963718257No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15960.1133848869880896No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15820.11239028271626426No Hit
GATAAGAACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAAA15600.11082733314625301No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15480.10997481519897413No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15180.10784352033077697No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15140.107559347681684No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15040.10684891605895162No Hit
GAACAATCGGCCCCCAGGGATCCCAGACCGATTCTCTGGCTCCCACTCTGGAAATACAGCTTCCTTGACCATCA14860.10557013913803331No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT14800.10514388016439388No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14790.10507283700212063No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCCGCTGTGT14740.10471762119075444No Hit
GAGTTACAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGT14730.10464657802848121No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14730.10464657802848121No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14550.1033678011075629No Hit
CAGTAATAACCACTATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC14530.10322571478301643No Hit
GGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCCGCTGTGTCTGTGGC14490.10294154213392347No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14470.102799455809377No Hit
GTGATGGTCAAGGAAGCTGTATTTCCAGAGTGGGAGCCAGAGAATCGGTCTGGGATCCCTGGGGGCCGATTGTT14400.1023021536734643No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14250.10123650623936571No Hit
GGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAAGTTTT14240.1011654630770925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)