FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995740
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCCAG44410.44599995982887103No Hit
GTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43890.4407777130576255No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40600.4077369594472453No Hit
CTCCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCC40420.4059292586418141No Hit
GTAATAAATAGCCGTGTCTGTGGTAATGAGAGACGTCATCTCCAGAGAAA39670.3983971719525177No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33770.339144756663386No Hit
ATTTATTACTGTGCGAGCCTCCCCCTCAAAAATGGGAACACTGACGAAGA31430.31564464619278126No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTAATGTGGCCCTCCTA26990.2710546929921465No Hit
GTATAGGAGGGCCACATTAGACGTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA26770.26884528089661963No Hit
GTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCATGGTACATACTGG26580.2669371522686645No Hit
GTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCACCC25990.26101191073975133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24810.24916142768192498No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22580.2267660232590837No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22340.22435575551850886No Hit
AGTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCACC22320.22415489987346093No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21590.21682366882921242No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21520.21612067407154478No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC21280.21371040633096994No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20270.20356719625605077No Hit
ATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20190.20276377367585918No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19570.19653724867937417No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19170.19252013577841603No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18680.1875991724747424No Hit
CTGTATAGGAGGGCCACATTAGACGTGCAGGAGATGGAGGCCGGCTGTCC18600.18679574989455078No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18570.18649446642697892No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18470.18549018820173943No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18370.18448590997649988No Hit
GTCAGACACTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATG17980.18056922489806576No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17900.17976580231787415No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17610.17685339546467954No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17200.1727358547411975No Hit
GGGTCAGACACTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGA16520.16590676280956876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16440.16510334022937714No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16420.16490248458432924No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16400.16470162893928134No Hit
GGCCAGTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTC15710.15777210918512866No Hit
CGTCTAATGTGGCCCTCCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCCAGGCCAATCTC15500.15566312491212567No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15130.15194729547873945No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15070.1513447285435957No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15000.15064173378592807No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGT14730.14793018257778134No Hit
GTGTGAACACAGTGTCTGACCCACTGCCGCTGAATCTGTCGGGGACCCCA14550.1461224817723502No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGACGGAGG14520.14582119830477835No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGTCTCCACTCTCC14340.14401349749934722No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14180.142406652338964No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGA14040.14100066282362866No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG13950.1400968124209131No Hit
CTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCATGGTACATA13750.13808825597043406No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCACCACAACATCG13640.1369835499226706No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13600.13658183863257475No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13540.13597927169743104No Hit
GTCCCAGCTCCATCTGCGGGAGTCGGGCCCACGACTGGTGAACCCTTCGGAGACCCTGTCCCTCACCTGCAGTGT13440.13497499347219155No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13410.13467371000461967No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13210.13266515355414066No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12980.13035531363608974No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12980.13035531363608974No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12850.12904975194327836No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12820.1287484684757065No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12800.1285476128306586No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTAATGTGGCCCTCCTATA12720.127744190250467No Hit
GTCTCTGGTGGCTCCATGACCAGCAGTGCTTCCTACTGGGTCTGGGTCCG12700.12754333460541908No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12570.1262377729126077No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12570.1262377729126077No Hit
TGGTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGG12450.1250326390423203No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12080.12131680960893407No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11980.12031253138369453No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11970.12021210356117058No Hit
GGTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGA11930.11981039227107478No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11910.11960953662602687No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11860.11910739751340711No Hit
GTGTCTGACCCACTGCCGCTGAATCTGTCGGGGACCCCAGAGTCCCGGTT11840.11890654186835921No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11580.11629541848273645No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGTCTCCACT11580.11629541848273645No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11490.11539156808002089No Hit
CTACAATCCATCCCTCCGGGATCGAGCCACCATATCCGTGGACACGTCCA11300.1134834394520658No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11250.11298130033944603No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11200.11247916122682627No Hit
GTCCTGTCCCAGCTCCATCTGCGGGAGTCGGGCCCACGACTGGTGAACCC11150.11197702211420651No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11120.11167573864663467No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11110.11157531082411072No Hit
CACCTGCAGTGTCTCTGGTGGCTCCATGACCAGCAGTGCTTCCTACTGGG11110.11157531082411072No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11100.11147488300158676No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11060.11107317171149096No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10920.10966718219615561No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTA10910.10956675437363167No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGGCCCCAGTAGTCTTCGTCAGTGTTCC10370.10414365195733825No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10370.10414365195733825No Hit
ACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGCTTTCTAACCGGGACTCTGGGGTCCCCGACAGATT10120.10163295639423946No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10080.10123124510414364No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10020.10062867816899992No Hit
GTATCAACAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAGGCTTTCTAACCGGGACTCTGGGGTCCCCGA9990.10032739470142808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)