FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995740
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCTGTGGTAATGAGAGACGTCATCTCCAGAGAAA40980.4115532167031554No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40190.4036194187237632No Hit
GTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT40050.4022134292084279No Hit
CTCCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCC38840.3900616626830297No Hit
CCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCCAG37280.37439492236929317No Hit
ATTTATTACTGTGCGAGCCTCCCCCTCAAAAATGGGAACACTGACGAAGA29600.297266354670898No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27680.2779842127462992No Hit
GTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCATGGTACATACTGG26690.26804185831642796No Hit
GTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCACCC25880.25990720469198786No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24870.24976399461706872No Hit
GTATAGGAGGGCCACATTAGACGTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA23690.23791351155924237No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22810.2290758631771346No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTAATGTGGCCCTCCTA22650.22746901801675135No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22050.22144334866531423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21620.21712495229678433No Hit
GTGTCTGACCCACTGCCGCTGAATCTGTCGGGGACCCCAGAGTCCCGGTT21130.21220398899311066No Hit
AGTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTCCACC20890.20979372125253581No Hit
GTGTGAACACAGTGTCTGACCCACTGCCGCTGAATCTGTCGGGGACCCCA20800.20888987084982025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19640.1972402434370418No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19220.19302227489103582No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18990.1907124349729849No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18340.18418462650892803No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18150.18227649788097294No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17730.17805852933496696No Hit
CTGTATAGGAGGGCCACATTAGACGTGCAGGAGATGGAGGCCGGCTGTCC17630.17705425110972745No Hit
ATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17430.17504569465924838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17020.17092815393576635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16720.1679153192600478No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16650.16721232450238013No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16630.16701146885733223No Hit
GGGTCAGACACTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGA16560.16630847409966457No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16260.163295639423946No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16110.16178922208608673No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15750.15817382047522446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15530.1559644083796975No Hit
GTCAGACACTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATG15520.15586398055717354No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15460.15526141362202983No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15440.15506055797698196No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15360.15425713539679034No Hit
GGCCAGTATGTACCATGCATGCAGTAATAAATGCCAACATCCTCAGCCTC15310.15375499628417058No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14920.14983831120573643No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGACGGAGG14750.14813103822282925No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14650.14712675999758973No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14580.14642376523992207No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14520.14582119830477835No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14380.14441520878944303No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14240.1430092192741077No Hit
CGTCTAATGTGGCCCTCCTATACAGTGATGGAAACACCTACGTGAATTGGTATCAACAGAGGCCAGGCCAATCTC14110.14170365758129633No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14110.14170365758129633No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13610.1366822664550987No Hit
CTGTGTTCACACTGCAAATCTCCAGGGTGGAGGCTGAGGATGTTGGCATTTATTACTGCATGCATGGTACATA13210.13266515355414066No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCACCACAACATCG13130.13186173097394902No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13080.13135959186132928No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGTCTCCACTCTCC13020.13075702492618557No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTAATGTGGCCCTCCTATA12870.12925060758832627No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGT12650.12704119549279932No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12280.12332536605941309No Hit
GTCTGACCCACTGCCGCTGAATCTGTCGGGGACCCCAGAGTCCCGGTTAG12210.12262237130174544No Hit
GTCTCTGGTGGCTCCATGACCAGCAGTGCTTCCTACTGGGTCTGGGTCCG12200.12252194347922149No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12110.12161809307650591No Hit
TGGTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGG12090.121417237431458No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11950.12001124791612269No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11890.11940868098097898No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGA11820.11870568622331132No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGGCCCCAGTAGTCTTCGTCAGTGTTCC11760.11810311928816761No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11650.11699841324040412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11560.11609456283768856No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11520.11569285154759273No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11410.11458814549982928No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11270.11318215598449394No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11120.11167573864663467No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11040.11087231606644304No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11030.11077188824391909No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10750.10795990921324844No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10710.10755819792315263No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10680.10725691445558076No Hit
GGTACATACTGGCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGA10610.1065539196979131No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10580.10625263623034126No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGCTGACCAGGGTTCCCTGG10570.1061522084078173No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10370.10414365195733825No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGCGATGTTGTGGTGACTCAGTCTCCACT10370.10414365195733825No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10360.1040432241348143No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCACGTCTA10310.10354108502219454No Hit
CACCTGCAGTGTCTCTGGTGGCTCCATGACCAGCAGTGCTTCCTACTGGG10300.10344065719967059No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10070.1011308172816197No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10060.10103038945909575No Hit
CTACAATCCATCCCTCCGGGATCGAGCCACCATATCCGTGGACACGTCCA10040.10082953381404786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)