FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107576
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGGAGAGGGTCACCATCTC52780.47653614740658884No Hit
GTAATATACGGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGGAGAAACA48560.4384349245559672No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47820.43175366746841753No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46820.4227249416744313No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT45890.4143282266860243No Hit
GCCTTGGGCTGACCTAGGATGGTCACCTTGGTCCCTCCGCCGAAAACCACACCATGGAGACTGTCATCCCATG44130.3984376692886087No Hit
GACCTAGGATGGTCACCTTGGTCCCTCCGCCGAAAACCACACCATGGAGA41580.37541441851394397No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38120.3441750272667519No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33540.30282346313029535No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGGAGAGGGTCAC32710.2953296207212869No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCACCTTGGTCCCTC31000.27989049961357054No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTCCATGGTGTGGTTTTCGG28080.25352662029513096No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27580.24901225739813793No Hit
GTATATTACTGTGCGAGAGATCAGACTATGATACCAGACTACTGGGGCCA27500.248289959334619No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26070.23537888144921884No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25180.22734331549257117No Hit
CCCCTGAGGGCAGCAGATCATCATAATAGATGAGGAGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCAATATA24710.2230998143693977No Hit
GGATGACAGTCTCCATGGTGTGGTTTTCGGCGGAGGGACCAAGGTGACCATCCTAGGTCAGCCCAAGGCTGCCCC24710.2230998143693977No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24090.21750200437712627No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23850.2153351101865696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23070.2082927040672604No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22440.20260460681704912No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGT22150.19998627633679317No Hit
GTGTTGTATATTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTATTATGATGATCTGCTGCCCT21620.19520105166598048No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21270.19204099763808535No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21050.19005467796340839No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20550.1855403150664153No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20140.18183853749088097No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGT19840.17912991975268516No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19310.17434469508187247No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19250.17380297153423332No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19250.17380297153423332No Hit
GTTGTATATTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCAT19190.17326124798659415No Hit
GGACTGAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19040.17190693911749624No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG18870.17037205573251857No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTTCCCCCTGAGCTGGGTCCGCCAGGCTCCACGAAAGGGG18870.17037205573251857No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18760.1693788958951801No Hit
CTCCTGTGCTGGAAGCGGCTCCAATATCGGAAGTAGTGTTGTATATTGGTACCAGCAGCTCCCAGGAAAGGCTCC18550.16748286347844302No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGGAGAGGGTCAC18240.1646839584823073No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGTCTCCATGGTGTGGTTTTCGGCGGAGGGAC18190.164232522192608No Hit
GAACAGGGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT18010.1626073515496905No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGC17950.16206562800205132No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17930.1618850534861716No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT17930.1618850534861716No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17810.16080160639089328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17760.16035017010119396No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17710.15989873381149464No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17570.1586347122003366No Hit
TCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAG17500.15800270139475756No Hit
GTATATTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAACTCCTCATCTA17430.15737069058917852No Hit
ACCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACAGTGTTTCTC17280.1560163817200806No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17050.1539397747874638No Hit
CCCTCGGTGTCTGCAGCCCCCAGGGAGAGGGTCACCATCTCCTGTGCTGG16760.15132144430720781No Hit
CTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCTCAGACTGGAG16380.14789052850549309No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15660.14138984593382303No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15580.14066754787030417No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15510.14003553706472513No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15450.13949381351708595No Hit
CTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15350.13859094093768734No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15310.1382297919059279No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15070.13606289771537122No Hit
CCTTGGTCCCTCCGCCGAAAACCACACCATGGAGACTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCT14980.13525031239391247No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14860.13416686529863414No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCACCTTGGTCCCTCCGCCGAAAACCACAC14800.13362514175099496No Hit
GCATGGGATGACAGTCTCCATGGTGTGGTTTTCGGCGGAGGGACCAAGGTGACCATCCTAGGTCAGCCCAAGG14750.13317370546129567No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14390.12992336417546063No Hit
ATATACAACACTACTTCCGATATTGGAGCCGCTTCCAGCACAGGAGATGGTGACCCTCTCCCTGGGGGCTGCAGA14260.12874962982224245No Hit
AGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGGAGAGG14210.12829819353254313No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGG13900.12549928853640743No Hit
TCATAATAGATGAGGAGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCA13880.12531871402052772No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13870.12522842676258786No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13860.125138139504648No Hit
ATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13760.12423526692524936No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13640.12315181982997105No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG13620.1229712453140913No Hit
CCCCAGGGAGAGGGTCACCATCTCCTGTGCTGGAAGCGGCTCCAATATCG13610.12288095805615144No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13440.1213460746711738No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13380.12080435112353463No Hit
GTATGTGGTACCACCACTGCCACTGATAGTTGAGACCCACTCCAGCCCCTTTCGTGGAGCCTGGCGGACCCAGCT13290.11999176580207588No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCAG13260.11972090402825629No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13170.11890831870679755No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13100.11827630790121851No Hit
GTATCAACGCAGAGTACGGGAGCTGCGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCC13030.11764429709563949No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12960.11701228629006045No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12890.11638027548448143No Hit
GTGCTGGAAGCGGCTCCAATATCGGAAGTAGTGTTGTATATTGGTACCAGCAGCTCCCAGGAAAGGCTCCCAAA12850.11601912645272199No Hit
CATCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12740.1150259666153835No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12710.11475510484156393No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12700.11466481758362407No Hit
GGGAGAGGGTCACCATCTCCTGTGCTGGAAGCGGCTCCAATATCGGAAGTAGTGTTGTATATTGGTACCAGCAG12690.11457453032568421No Hit
CCTTTAGTAGCTTCCCCCTGAGCTGGGTCCGCCAGGCTCCACGAAAGGGG12680.11448424306774435No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGATGGTCACCTTGGTC12590.11367165774628558No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12560.113400795972466No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12550.11331050871452614No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12510.1129493596827667No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12260.11069217823427016No Hit
CCATGGAGACTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCTC12160.10978930565487154No Hit
GGACCAAGGTGACCATCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12030.10861557130165335No Hit
CCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGTC11830.10680982614285611No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11790.10644867711109667No Hit
CAGTAATATACGGCCGTGTCCCCGGCTCTCAGGCTGTTCATTTGGAGAAA11730.1059069535634575No Hit
GTCTGTGCTGACTCAGCCACCCTCGGTGTCTGCAGCCCCCAGGGAGAGGGTCACCATCTCCTGTGCTGGAAGCGG11710.10572637904757778No Hit
ATACAACACTACTTCCGATATTGGAGCCGCTTCCAGCACAGGAGATGGTGACCCTCTCCCTGGGGGCTGCAGACA11650.1051846554999386No Hit
CTATTATGATGATCTGCTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC11580.10455264469435958No Hit
CTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGT11550.10428178292053998No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGAGACCCCTGAGGGCAGCAG11500.10383034663084068No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTTCCCCCTGAGCTGGGTCCG11460.10346919759908124No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11400.10292747405144208No Hit
ATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCGCTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11390.10283718679350219No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11370.10265661227762247No Hit
ACCTAGGATGGTCACCTTGGTCCCTCCGCCGAAAACCACACCATGGAGAC11370.10265661227762247No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11360.10256632501968262No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCTGGTATCATAGT11300.10202460147204345No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11290.1019343142141036No Hit
GTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCAATTGGTGATCGGGACTGAACAGGGAGAACTCA11150.10067029260294554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)