FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570418
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97890.623337226139792No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90030.573286857384467No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC87160.5550114682842402No Hit
GCTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT84430.5376275615791465No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC68210.4343429583715928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61410.39104238489370347No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55890.3558925075998874No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAG55190.3514350956242223No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG54680.34818755261338064No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54650.34799652067156644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT54280.34564046005585775No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTT54000.34385749526559173No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG49780.31698566878372514No Hit
GATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA49020.3121461929244316No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC47520.30259459583372067No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG47030.29947440745075515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45480.2896044237903539No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44370.28253624194322785No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44000.28018018132751915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42650.27158374394587936No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT42600.271265357376189No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42400.2699918110974276No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT42210.2687819421326042No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41880.2666805907726478No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG41780.26604381763326707No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41600.26489762598238176No Hit
ATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA41430.26381511164543453No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38580.24566707717308386No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT37290.23745270367507249No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36780.2342051606642308No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTAC34230.2179674456100223No Hit
GCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGC32650.20790643000780684No Hit
ACCATATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAATACACTGTATCTG32340.20593243327572658No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA32240.20529566013634584No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31310.19937366994010514No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC30940.19701760932439644No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30780.19599877230138726No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC30460.19396109825536895No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30080.1915413603257222No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30070.1914776830117841No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC29430.18740233491974748No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA29360.18695659372218099No Hit
GTATATGGTATCACCATTAGGACTAATAGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCA28540.18173505397925904No Hit
AGTCAGGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTAAGTTCCTGATCTATG28500.18148034472350674No Hit
CTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTG28290.18014312113080722No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28000.17829647902660312No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27960.17804176977085082No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27300.17383906705093802No Hit
GTTCCTGATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGT25930.1651152750414221No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25220.16059418575181894No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT24910.15862018901973868No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24760.15766502931066761No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC24500.15600941914827773No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24500.15600941914827773No Hit
GATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAA24390.15530896869495892No Hit
ATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAAAACTATTAATGTC24380.15524529138102083No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24110.1535260039046929No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG23530.1498327196962847No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23510.14970536506840854No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23390.14894123730115166No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGG23290.14830446416177095No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG23280.14824078684783287No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23120.1472219498248237No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23080.1469672405690714No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22880.14569369429030996No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22880.14569369429030996No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22430.1428282151630967No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT22410.14270086053522058No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG22110.1407905411170784No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22070.1405358318613261No Hit
ACCTTGATGGGACCCCACTTTGCAGAGTGGATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTG21920.139580672152255No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21900.13945331752437887No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCG21880.13932596289650273No Hit
ATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA21870.13926228558256465No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21760.13856183512924583No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21280.13550532406021834No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21240.13525061480446607No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21090.134295455095395No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20840.13270352224694315No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20510.13060217088698678No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC19940.12697256399251663No Hit
GCCCCTAAGTTCCTGATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT19770.1258900496555694No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19730.12563534039981714No Hit
GGCCGAAGGTGATCGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCA19720.12557166308587903No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19590.12474385800468411No Hit
GAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAG19470.12397973023742723No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19310.12296089321441807No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19140.12187837887747083No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19130.12181470156353276No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT18910.12041380065689518No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18820.11984070483145252No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18790.11964967288963829No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18670.11888554512238143No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT18590.11837612661087685No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGTCACATCTGGCACCTGGGAGCCAGAG18490.11773935347149612No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18440.11742096690180576No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18330.11672051644848697No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT18230.11608374330910624No Hit
CATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTAAGTTCCTGATCTATGCTGCATCCA18180.11576535673941588No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCGATCAC18120.11538329285578745No Hit
GTGGATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTG18110.11531961554184936No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18090.11519226091397322No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18010.11468284240246863No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC17990.1145554877745925No Hit
ACTTTGCAGAGTGGATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTG17980.11449181046065443No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17860.11372768269339754No Hit
GGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTAAGTTCCTGATCTATGCTGCATC17810.11340929612370719No Hit
GGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATT17710.11277252298432647No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17690.11264516835645033No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17130.10907923877591826No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16810.10704156472989995No Hit
CTGTAGGAGACAGAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAAT16760.10672317816020958No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCGATCACCTT16750.10665950084627149No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG16670.10615008233476693No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16500.10506756799781969No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16250.10347563514936788No Hit
CTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGA16110.10258415275423485No Hit
CTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAAAACTATTAA15940.10150163841728763No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA15910.10131060647547341No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGTAACTATTAAGCTGTT15890.10118325184759727No Hit
TCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGAGC15770.10041912408034039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)