FastQCFastQC Report
Wed 8 May 2024
EGAF00004691812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570418
Total Bases116.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT105750.6733875948951171No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC96230.6127667920260721No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT90830.5783810424995128No Hit
GCTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTA83630.5325333764641007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC71550.455611181226909No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC69460.44230262261385184No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC63280.402950042600123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT62560.39836527599658184No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54210.3451947188582912No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAATAG54190.34506736423041506No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTT51620.32870229454833044No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG50290.3202332117945668No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA50120.31915069745761954No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA49560.3155847678770875No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC49300.31392915771469765No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA48160.30666994392575736No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT47880.3048869791354913No Hit
GATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCG47440.3020851773222161No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG47320.30132104955495925No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT46630.2969273148932322No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT44830.2854653983843792No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG44580.28387346553592735No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG44040.2804348905832715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43050.2741308365034023No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC42450.2703101976671179No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC42410.2700554884113656No Hit
ATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT39750.25311732290383837No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT39610.25222584050870533No Hit
ACCATATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA39320.25037919840450124No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT39170.24942403869543014No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC38890.2476410739051641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38840.24732268733547372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG36310.23121232690914137No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA35110.2235710492365727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC33210.21147235958833888No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA32530.20714230224054994No Hit
GCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGC32190.20497727356665552No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT32170.20484991893877938No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC31300.19930999262616705No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC31290.199246315312229No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31260.19905528337041475No Hit
CTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGA31160.19841851023103402No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA29830.18994942747727037No Hit
ACTTATTACTGTCAACAGCTTAATAGTTACCCGATCACCTTCGGCCAAGG29040.18491891967616264No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC28720.18288124563014432No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC28650.18243550443257783No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA28610.18218079517682553No Hit
GTATATGGTATCACCATTAGGACTAATAGATGAGACCCACTCCAGCCCCT28530.18167137666532096No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC28530.18167137666532096No Hit
GTTCCTGATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCCATCAAGGT27840.17727764200359394No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27560.17549467721332793No Hit
AGTCAGGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAA27240.1734570031673096No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26270.16728030371531655No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC25850.1646058565299175No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGGTAACTATTAAGCTGTTGACAG25820.1644148245881033No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG25790.16422379264628909No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT25760.16403276070447487No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25730.16384172876266065No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA25110.15989373529850015No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25080.15970270335668593No Hit
GATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAA25060.15957534872880977No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25010.15925696215911941No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT24960.15893857558942906No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24470.1558183872064635No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC24120.15358968121863098No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA24030.1530165853931883No Hit
ATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTGATAC23730.15110626597504614No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23680.15078787940535576No Hit
ATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA23610.15034213820778924No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT23470.14945065581265624No Hit
ACCTTGATGGGACCCCACTTTGCAGAGTGGATGCAGCATAGATCAGGAAC23260.1481134322199567No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC22870.1456300169763719No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22850.14550266234849576No Hit
GGCCGAAGGTGATCGGGTAACTATTAAGCTGTTGACAGTAATAAGTTGCA22480.14314660173278707No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22390.1425735059073444No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT22300.14200041008190176No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC22160.14110892768676875No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22090.14066318648920223No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATC22080.14059950917526418No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA21680.13805241661774126No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21590.1374793207922986No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21060.13410442315358076No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC21050.1340407458396427No Hit
GTGGATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTGGCTTTTGCTG20700.13181203985181017No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20520.13066584820092486No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA20280.12913759266641112No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20240.12888288341065882No Hit
GCCCCTAAGTTCCTGATCTATGCTGCATCCACTCTGCAAAGTGGGGTCCC20210.1286918514688446No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20160.12837346489915422No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20070.1278003690737116No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20040.12760933713189737No Hit
GTCTCCTACAGATGCAGACAGGAAGGATGGAGACTGGGTCAACTGGATGT20020.12748198250402124No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19760.12582637234163133No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19460.12391605292348916No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCG19410.12359766635379879No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19340.1231519251562323No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAGAGT19330.1230882478422942No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCA19190.12219676544716121No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19110.12168734693565662No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTC19040.1212416057380901No Hit
GAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAATAGTTTTTTAGCC18980.12085954185446167No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18740.11933128631994794No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18600.11843980392481492No Hit
GGACATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCC18510.11786670809937227No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18080.11512858360003515No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGGTAACTATTAAGCTGTT18050.11493755165822094No Hit
ACTTTGCAGAGTGGATGCAGCATAGATCAGGAACTTAGGGGCTTTCCCTG18010.11468284240246863No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCA17900.11398239194914984No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17840.11360032806552141No Hit
CATTAATAGTTTTTTAGCCTGGTATCAGCAAAAGCCAGGGAAAGCCCCTA17780.11321826418189299No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG17440.11105323550799852No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17320.11028910774074163No Hit
GGTAACTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGC17310.11022543042680356No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT17230.10971601191529898No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA17120.10901556146198019No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17070.10869717489228982No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT17010.1083151110086614No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16960.10799672443897103No Hit
CAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGT16710.10640479159051922No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG16700.10634111427658113No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG16690.10627743696264307No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16590.10564066382326234No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16440.10468550411419125No Hit
CTGTAGGAGACAGAGTCACCATCGCTTGCCGAGCCAGTCAGGACATTAAT16400.10443079485843897No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA16250.10347563514936788No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTTAGCAGCTATAGCATGTGCTGGG16200.10315724857967751No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16120.10264783006817292No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGAGCCAGTCAGG16000.10188370230091606No Hit
CTTAGGGGCTTTCCCTGGCTTTTGCTGATACCAGGCTAAAAAACTATTAA15990.101820024986978No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT15980.10175634767303993No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15800.10061015602215462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)