FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945134
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC136990.7042702456488859No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC100720.5178049430013562No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT73160.3761180463659573No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG66030.33946247405063096No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG60600.3115466595103474No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC57710.2966890712927747No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT56470.2903141891509788No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC56440.2901599581314192No Hit
CCTATGAATTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA54910.28229417613388075No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACAGTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAG52840.2716522357842699No Hit
GACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAG47490.24414770396281182No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT47290.24311949716574796No Hit
GTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG46950.24137154561073942No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG46300.2380298735202819No Hit
GTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG46000.2364875633246861No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAGTTGGGGGAT45970.23633333230512654No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG45890.235922049586301No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG45890.235922049586301No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG43380.22301805428314964No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT43140.22178420612667302No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG42840.22024189593107726No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC42320.21756855825871124No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC41080.21119367611691534No Hit
CCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCT40940.21047393135897063No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA40780.20965136592131955No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40490.20816046606557695No Hit
GTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCTGAGCG40130.20630969383086203No Hit
GGATACGAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCAT39470.2029166114005513No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39460.2028652010606981No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT38550.19818686013405762No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT37710.1938683915863894No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA37510.19284018478932557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG37390.19222326071108725No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTGTGGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACA35200.18096439628323807No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34830.17906221370866993No Hit
AGCCAGGCCAGTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCC34580.17777695521234013No Hit
GGATCCGTGGCCTCCTATGAATTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC34360.1766459277355699No Hit
CTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCC34210.17587477263777201No Hit
AGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCC33040.16985976287494847No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32660.16790616996052715No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG32580.16749488724170158No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT32290.166003987385959No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC31930.1641532151512441No Hit
CTTCGTATCCTGATAGATGACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCCCC31750.1632278290338866No Hit
CAGTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGT31150.16014320864269505No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC30660.1576241019898886No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT30220.15536204703634815No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29620.1522774266451566No Hit
ACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCC29290.15058088543000123No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29220.15022101305102886No Hit
CGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCA29200.15011819237132248No Hit
GCACAGGATCCGTGGCCTCCTATGAATTGACTCAGCCACCCTCAGTGTCC29080.1495012682930842No Hit
GACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGT28510.14657087892145218No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC28250.1452342100852692No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC27910.14348625853026065No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27790.14286933445202232No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAATTGACT27320.1404530484789223No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26720.1373684280877307No Hit
GCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCC26500.13623740061096049No Hit
GGAGATAAGTTGGGGGATAACTATGTTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTCTTGGTCATCTAT26370.13556906619286896No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25640.1318161113835859No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25550.13135341832490718No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC24920.12811456691415604No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC24840.1277032841953305No Hit
GGCCAGTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGG24430.1255954602613496No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24150.1241559707454602No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG23930.12302494326868997No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23830.12251083987015804No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23780.12225378817089208No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC23570.12117417103397504No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT23330.11994032287749841No Hit
GGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCCCCCAACTTATCTCCAGAGCAGGTGATGCTGGCTGT23220.11937480913911329No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG23210.1193233987992601No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC22920.11783249894351752No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22880.11762685758410474No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22780.11711275418557282No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACAGTGTAGT21990.1130513373371706No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21710.11161184782128121No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG21520.11063505136407056No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC21400.11001812728583223No Hit
GGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG21300.1095040238873003No Hit
GTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT21260.10929838252788755No Hit
GGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGT21260.10929838252788755No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC21220.10909274116847477No Hit
GCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC21170.1088356894692088No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA20980.10785889301199814No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20740.10662504485552153No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20690.10636799315625554No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA20580.10580247941787044No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20530.10554542771860446No Hit
GACCCAGGCTGTGGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGAC20430.10503132432007256No Hit
CCACAGTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAGCCTCATCCACA20420.10497991398021936No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC20120.10343760378462358No Hit
GCTTGGTCCCTCCGCCGAATGCCACAGTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAGCCTCATCCACAGC20110.1033861934447704No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT19940.10251221766726612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)