FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945134
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC127260.6542479849717294No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC94680.48675309773002784No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG73160.3761180463659573No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69450.35704481028042284No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC59070.30368087751280887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57070.29339880954217035No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG55730.28650982400184255No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG53170.2733487769994252No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT52450.2696472325299954No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC51380.2641463261657037No Hit
CCTATGAATTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA50170.2579256750434674No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG48060.24707809333444378No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT47630.24486744872075653No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACAGTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAG46490.23900666997749256No Hit
GACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAG45320.23299166021466902No Hit
GTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG44410.22831331928802848No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGG43600.22414908175991988No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAAGTTGGGGGAT43230.2222468991853518No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42530.21864817539562825No Hit
CCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCT42410.21803125131738993No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC40930.21042252101911743No Hit
GTGTAGTCGCCCCACGCCTGACAGTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGG40690.2091886728626408No Hit
GTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCTGAGCG40580.20862315912425572No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC40090.20610405247144928No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT39560.20337930445923005No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39530.20322507343967047No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA38580.19834109115361717No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38440.19762134639567247No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38220.19649031891890226No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG37610.1933542881878575No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT37280.19165774697270213No Hit
GGATACGAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGACCAT37270.19160633663284896No Hit
GGATCCGTGGCCTCCTATGAATTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAC35220.18106721696294445No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34620.17798259657175292No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC34470.177211441473955No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34230.1759775933174784No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA32590.1675462975815548No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA32290.166003987385959No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC32250.16579834602654625No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTGTGGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACA32090.16497578058889517No Hit
CTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCC31720.16307359801432703No Hit
GCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGG31480.1618397498578504No Hit
AGCCAGGCCAGTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCC31200.160400260341961No Hit
AGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCC31150.16014320864269505No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT31000.15937205354489717No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC30870.15870371912680567No Hit
CTTCGTATCCTGATAGATGACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCCCC30860.15865230878695247No Hit
GACCAAGACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATAGT28960.14888434421484587No Hit
CAGTAGTAGTCAGCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGT27790.14286933445202232No Hit
GCACAGGATCCGTGGCCTCCTATGAATTGACTCAGCCACCCTCAGTGTCC27740.14261228275275636No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27640.14209817935422445No Hit
ACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCC27610.14194394833466487No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC27420.14096715187745418No Hit
CGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCA27150.139579072701418No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26910.13834522454494136No Hit
GGAGATAAGTTGGGGGATAACTATGTTTGCTGGTATCAGCAGAAGCCAGGCCAGTCCCCTGTCTTGGTCATCTAT26820.13788253148626264No Hit
GCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCC26600.1367515040094924No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG26540.13644304197037324No Hit
GCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTGCACAGGATCCGTGGCCTCCTATGAATTGACT26400.13572329721242854No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC25490.13104495628578802No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25380.1304794425474029No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT25210.12960546676989862No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25110.1290913633713667No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC25040.12873149099239437No Hit
GGCCAGTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGG24410.12549263958164322No Hit
GGCCTGGCTTCTGCTGATACCAGCAAACATAGTTATCCCCCAACTTATCTCCAGAGCAGGTGATGCTGGCTGT23880.12276789156942401No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22890.11767826792395794No Hit
GGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGACCAAGCTGACCGT22530.115827495689243No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22430.11531339229071108No Hit
GCCTCAGCCATGGCATGGATCCCTCTCTTCCTCGGCGTCCTTGCTTACTG22430.11531339229071108No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA22250.11438800617335361No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22180.11402813379438126No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22070.11346262005599614No Hit
GTCCCCTGTCTTGGTCATCTATCAGGATACGAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACT22060.11341120971614296No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACAGTGTAGT21850.1123315925792259No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21790.11202313054010675No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC21790.11202313054010675No Hit
GTGTCCGTGTCCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATAA21420.11012094796553863No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21300.1095040238873003No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG20700.10641940349610875No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT20560.10569965873816405No Hit
GCCTCATCCACAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCC20400.10487709330051297No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20340.1045686312613938No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC20250.10410593820271508No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC20210.1039002968433023No Hit
GGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG20190.10379747616359593No Hit
GACCCAGGCTGTGGATGAGGCTGACTACTACTGTCAGGCGTGGGGCGACTACACTGTGGCATTCGGCGGAGGGAC20050.10307773140565123No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC20010.10287209004623847No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC19720.10138119019049588No Hit
GTATCAACGCAGAGTACGGGGGGCCTCAGGAAGCAGCATCGGAGGTGCCTCAGCCATGGCATGGATCCCTCTCTT19550.1005072144129916No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19470.10009593169416604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)