FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659690
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40990.6213524534250936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33810.5125134532886659No Hit
GAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT29750.4509693947156998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27520.41716563840591797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26320.398975276266125No Hit
GTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAATTTGGTATCAGCAGAAA22820.3459200533583956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22290.33788597674665377No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22220.3368248722884991No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21670.3284876229744274No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21230.32181782352317No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20900.3168154739347269No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20450.30999408813230456No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19920.30196001152056273No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGCTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC19470.2951386257181403No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAA19400.29407752125998576No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19220.29134896693901685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18430.27937364519698643No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17940.2719459139899043No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC17920.2716427412875745No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17880.2710363958829147No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG17760.2692173596689354No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17050.2584547287362246No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16960.2570904515757401No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16910.2563325198199154No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAA16730.2536039654989465No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC16690.2529976200942867No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC15910.24117388470342133No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15380.23313980809167942No Hit
GTAGTAAGTTGCAAAATCGTCAGGCTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15340.23253346268701963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14810.22449938607527778No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14330.21722324121936062No Hit
CACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTT13880.21040185541693826No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13430.2035804696145159No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13210.2002455698888872No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13180.1997908108353924No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12400.18796707544452695No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12310.1866027982840425No Hit
GTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGA11980.18160044869559944No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11960.18129727599326956No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11860.17978141248162016No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11780.17856872167230062No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11620.17614334005366158No Hit
GACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11330.17174733586987825No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11250.17053464506055876No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11240.1703830587093938No Hit
AGTCAGAGCATTAGCACCTATTTAATTTGGTATCAGCAGAAACCAGGGAA11200.16977671330473404No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11140.1688671951977444No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11080.16795767709075476No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11070.1678060907395898No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC10940.16583546817444558No Hit
GTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGA10700.16219739574648698No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10680.1618942230441571No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10650.16143946399066228No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAATAGGTGCTA10600.16068153223483758No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10600.16068153223483758No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10540.15977201412784794No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10400.15764980521153876No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA10360.157043459806879No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10230.15507283724173476No Hit
CTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTT10200.15461807818823994No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10120.1534053873789204No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10020.151889523867271No Hit
CCCCTAAACTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCA9980.15128317846261122No Hit
ACCTTGATGGGACCCCACCTTGCAAAGTGGATGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9870.14961572859979688No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9760.14794827873698252No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9690.14688717427882794No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTA9610.14567448346950843No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTG9610.14567448346950843No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCG9580.14521972441601358No Hit
ATTTAATTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC9570.14506813806484864No Hit
GCATTAGCACCTATTTAATTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCATCCA9540.14461337901135382No Hit
GTGGATGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAATAGGTGCTAATG9520.14431020630902394No Hit
GATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9510.144158619957859No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9410.14264275644620958No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9380.14218799739271476No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9380.14218799739271476No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9280.14067213388106534No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9180.13915627036941594No Hit
TCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAAT9080.13764040685776655No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9080.13764040685776655No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9010.13657930239961194No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATT8870.13445709348330276No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8740.13248647091815852No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGG8730.13233488456699358No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8710.1320317118646637No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8660.131273780108839No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8480.12854522578787006No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8410.12748412132971548No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT8350.12657460322272582No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8330.12627143052039594No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8250.12505873971107642No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT8220.12460398065758159No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8210.12445239430641665No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGG8210.12445239430641665No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAAT8190.12414922160408678No Hit
GTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCA8070.12233018539010748No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8050.12202701268777759No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTT7980.120965908229623No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7940.12035956282496324No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7910.11990480377146841No Hit
GCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGG7860.11914687201564371No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACCTTGCAAAGT7770.11778259485515925No Hit
TTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTG7580.11490245418302536No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7470.11323500432021101No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCACCTGGATGT7430.11262865891555124No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC7400.11217389986205642No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7380.11187072715972653No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC7270.11020327729691218No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7200.10914217283875759No Hit
GGACCCCACCTTGCAAAGTGGATGCATCATAGATCAGGAGTTTAGGGGCT7170.10868741378526278No Hit
GGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC7080.1073231366247783No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7000.10611044581545878No Hit
ACTGTCAACAGAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTG6900.10459458230380936No Hit
ATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGA6890.10444299595264443No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6880.10429140960147949No Hit
TCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT6740.10216920068517031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)