FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659690
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40690.6168048628901455No Hit
GAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCAT27230.4127696342221346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25440.3856356773636102No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25430.38548409101244524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25360.3844229865542907No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23890.3621397929330443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23310.3533477845654777No Hit
GTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAATTTGGTATCAGCAGAAA21850.3312161772953963No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19760.29953462990192364No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19710.2987766981460989No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGCTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC18860.28589185829707897No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18770.28452758113659443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18690.2833148903272749No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC18280.2770998499295123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18180.2755839864178629No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17870.27088480953174976No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17660.267701496157286No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGGTCAAACGAA17590.2666403916991314No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17400.2637602510269975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17210.2608801103548637No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTG17150.259970592247874No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16400.2486016159105034No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCC16350.24784368415467872No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15900.2410222983522564No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTC15770.23905167578711214No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15520.2352620170079886No Hit
GTAGTAAGTTGCAAAATCGTCAGGCTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15250.23116918552653518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAA15190.23025966741954557No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15170.22995649471721566No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14830.2248025587776077No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14800.22434779972411284No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14600.22131607270081405No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13450.20388364231684578No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12930.19600115205626886No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12810.19418211584228956No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12740.19312101138413498No Hit
CACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTT12620.19130197517015568No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12600.1909988024678258No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12300.18645121193287756No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11970.18144886234443452No Hit
GTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCGGGCAAGTCAGA11780.17856872167230062No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11740.17796237626764086No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11430.1732631993815277No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11230.17023147235822886No Hit
GTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCCTCTCACTTTCGGCGGAGGGA10990.16659339993027028No Hit
GACCAAGGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10960.16613864087677546No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10880.16492595006745592No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCC10740.16280374115114674No Hit
CTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTT10670.16174263669299216No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10580.1603783595325077No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAATAGGTGCTA10410.1578013915627037No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10400.15764980521153876No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10330.15658870075338416No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10160.15401173278358016No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9990.15143476481377616No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA9770.14809986508814746No Hit
GATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA9760.14794827873698252No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT9760.14794827873698252No Hit
AGTCAGAGCATTAGCACCTATTTAATTTGGTATCAGCAGAAACCAGGGAA9630.14597765617183828No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9620.14582606982067334No Hit
GTGGATGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAATAGGTGCTAATG9560.1449165517136837No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9370.14203641104154982No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9320.1412784792857251No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9310.14112689293456018No Hit
CCCCTAAACTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCA9270.1405205475299004No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTGCAACTTA9250.14021737482757055No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9230.13991420212524064No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9190.13930785672058088No Hit
ACCTTGATGGGACCCCACCTTGCAAAGTGGATGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9160.13885309766708606No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9130.13839833861359124No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9120.1382467522624263No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9060.13733723415543664No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9050.1371856478042717No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCACTTGCCG8880.1346086798344677No Hit
ATTTAATTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATC8800.13339598902514818No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8730.13233488456699358No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATTTTG8720.13218329821582867No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGG8700.13188012551349876No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8610.13051584835301427No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8610.13051584835301427No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACCTTGCAAAGT8560.12975791659718958No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8550.12960633024602464No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8490.128696812139035No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAGCCTGACGATT8490.128696812139035No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8430.12778729403204533No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8310.12596825781806606No Hit
GCATTAGCACCTATTTAATTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCATCCA8210.12445239430641665No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8160.12369446255059194No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8010.12142066728311783No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC7970.12081432187845806No Hit
GCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTT7830.11869211296214889No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7790.11808576755748912No Hit
TCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAAT7790.11808576755748912No Hit
GTGCCAGATGTGACATCCAGGTGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCGCCATCA7720.11702466309933453No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT7690.11656990404583971No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7580.11490245418302536No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7470.11323500432021101No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7460.11308341796904607No Hit
TTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTG7440.11278024526671618No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT7430.11262865891555124No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7380.11187072715972653No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCACCTGGATGT7370.11171914080856159No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7310.11080962270157194No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7280.11035486364807713No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7210.10929375918992254No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAAATTAAAT7210.10929375918992254No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGAGGGGAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCGTCAGG7150.10838424108293289No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7010.10626203216662372No Hit
GTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGCGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGA6910.1047461686549743No Hit
ATGCATCCACTTTGCAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGA6790.10292713244099501No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT6690.1014112689293456No Hit
GGACCCCACCTTGCAAAGTGGATGCATCATAGATCAGGAGTTTAGGGGCT6680.10125968257818066No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG6620.10035016447119101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA252.528723E-455.36527341
TAGGTCG252.7668106E-454.3659636
GTATCAA17750.039.8505671
ACGCTTA350.001338346639.4825940
ATGCACG350.001378907539.24353836
GTGGTAT1300.039.2389561
TATCAAC19600.035.891142
GACAGGT952.564775E-1035.7969441
GCTTAAC400.002535519234.66487542
GCACGCT400.002612440634.45428538
TCAACGC20500.034.1418884
ATCAACG20500.034.1392973
GTTATAC400.002785275534.0070951
CCGGGTC502.0310305E-433.98903713
CAACGCA20800.033.6520085
AACGCAG21350.032.7850956
GTGTACG450.003807663531.90426462
CGCAGAG23050.030.6619328
CACGCTT450.00462511230.66139839
ACGCAGA22900.030.5660157
Produced by FastQC (version 0.11.5)