FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287463
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT133961.0404959210478282No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA121390.94286204729767No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC110830.8608402726913317No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT96370.74852636541788No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71170.5527925851073001No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC65040.5051795663254012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC62280.4837420570532901No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA58830.45694517046315114No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56440.4383815301876637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52050.40428346290339995No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51850.4027300202025223No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT51050.3965162493990118No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT50170.38968110151515034No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46980.36490369043615234No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG45280.3516994274786926No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT44460.3453303124050944No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43290.33624267260496027No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAA43240.3358543119297409No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43160.3352329348493899No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42170.32754339348004563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41190.31993152424574534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG40780.3167469667089462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39920.31006716309517246No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39640.3078923433139438No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39100.3036980480215742No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC38230.29694057227275655No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC37610.29212489990003593No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC35960.2793089976177956No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35960.2793089976177956No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35200.2734059153544607No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGATCCCCGGGT32260.2505703076515597No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA32050.2489391928156382No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32030.24878384854555044No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG31050.24117197931125012No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA30210.2346475199675641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29800.23146296243076503No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT29550.229521159054668No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28790.22361807679133305No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28770.22346273252124527No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28100.21825869947330526No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27990.21740430598782257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27180.2111128630492682No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26530.20606417427141593No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26470.20559814146115266No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTA26340.20458840370558223No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA26230.20373401022009954No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25880.2010154854935637No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACC25660.19930669852259833No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC25440.19759791155163292No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG25050.1945686982849216No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG24930.19363663266439501No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24720.19200551782847353No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24650.19146181288316635No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24380.18936466523698156No Hit
AGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA24140.18750053399592845No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23910.18571407488991917No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23530.1827625337582517No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23340.18128676319241796No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23240.18051004184197914No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23130.17965564835649644No Hit
GGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTT22780.1769371236299606No Hit
ATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGA22420.17414092676838092No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22170.1721991233922839No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTA22160.17212145125724002No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21930.17033499215123074No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21570.16753879528965104No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21550.16738345101956328No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21200.16466492629302745No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21170.16443190988789583No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTT21120.16404354921267641No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20570.15977158178526296No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20480.15907253256986803No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20330.15790745054420982No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20240.1572084013288149No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20060.15581030289802505No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAATAAGAACTA19890.15448987660227906No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19830.15402384379201578No Hit
CTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATC19760.1534801388467086No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19500.1514606633355677No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG19460.1511499747953922No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19170.14889748287911964No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18850.14641197455771546No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG18800.14602361388249605No Hit
CAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA18760.1457129253423205No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18670.1450138761269256No Hit
ACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATT18590.14439249904657456No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18480.14353810556109187No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC18460.1433827612910041No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18450.1433050891559602No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18370.14268371207560915No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18320.14229535140038976No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18190.1412856136448193No Hit
AATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGA17950.13942148240376617No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCA17920.13918846599863452No Hit
GTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTA17150.1332077116002557No Hit
CCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17110.13289702306008017No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16830.1307222032788515No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCC16760.13017849833354436No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16690.1296347933882372No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16600.12893574417284226No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16560.12862505563266674No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG16540.12846971136257895No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16460.12784833428222792No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16370.12714928506683298No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGT16200.12582885877108702No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT16100.12505213742064822No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16050.12466377674542879No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTCTTAGGAGTAGT15990.12419774393516551No Hit
GAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGC15920.12365403898985834No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTAC15380.11945974369748878No Hit
GCTTGGTACCAGCAAAAATCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTC15360.11930439942740101No Hit
GCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15340.11914905515731325No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC14940.11604216975555802No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14940.11604216975555802No Hit
TTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGA14910.11580915335042638No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14710.11425571064954876No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14680.11402269424441712No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14490.11254692367858338No Hit
GTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC14410.11192554659823234No Hit
GCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGT14330.11130416951788129No Hit
GGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATC14270.11083813670761801No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG14000.10874098906143322No Hit
GTCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTC13930.10819728411612604No Hit
GTATCAACGCAGAGTACGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGA13770.10695452995542395No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGCTGCAGACCCAGGTCTTCATTTCTCTGTTG13740.10672151355029232No Hit
GGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACA13720.10656616928020457No Hit
GCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTT13660.10610013646994128No Hit
GACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC13470.10462436590410752No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA13390.1040029888237565No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGC13310.10338161174340543No Hit
GTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG13220.10268256252801052No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC13180.10237187398783498No Hit
CATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCA13160.10221652971774724No Hit
GCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAG13140.10206118544765945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)