FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338860
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTGGATACGTCACCTCTGACGTTCGGTCAAGGGA57650.4305902036060529No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44340.331177270214959No Hit
CAGTAATACACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41310.3085460765128542No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36500.27261999014086613No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34220.25559057705809424No Hit
GTGTTAGTAGCGTCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGCTGCAT34180.25529181542506313No Hit
GTGTATTACTGTCAGCAGTTGGATACGTCACCTCTGACGTTCGGTCAAGGGACCAAGGTGGAGATCAAACGAA28650.21398801965851547No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28460.2125689019016178No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26710.19949808045650777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24740.18478407002972677No Hit
GCCTTATATTATTGTGCAAAAGCTCGCCGATTTTACAGCTCGTCCTTGTT24650.18411185635540683No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23890.1784353853278162No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23850.1781366236947851No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23360.1744767936901543No Hit
GTAATACACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22820.17044351164423466No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT22210.1658873967405106No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22170.1655886351074795No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCGTCTACTTAGCCTGGTACC22050.16469235020838624No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT21720.1622275667358798No Hit
ATATAAGGCCGTGTCCTCACGTCTCAGACTGTTCATTTCCAGCTGCAGGG21270.15886649836428005No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21260.15879180795602227No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21030.15707392856609353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20650.15423569305229823No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20360.15206967121282286No Hit
CTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA20110.15020241100637854No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19750.1475135563090988No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19000.14191177568976593No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCGACTGGTGGAGT18900.1411648716071882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18700.13967106344203278No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGACCGAACGTC18650.1392976114007439No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG18620.1390735401759706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18330.13690751833649523No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18300.13668344711172192No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT17990.134368044455731No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17830.13317299792360665No Hit
CTCCAGGCCCTTCCCTGGGGCTTGCCGGACCCAATGGAAGGCATAACCTT17680.13205264179974008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17290.12913971587768697No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGACGCTACTAACACTCTGA17250.1288409542446559No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16740.12503174342350956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16580.1238366968913852No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16090.1201768668867544No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16060.11995279566198108No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15980.11935527239591891No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15920.11890712994637229No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTGTATTACTGTCAGCAGTTGGATACGTCA15910.11883243953811451No Hit
GTCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC15790.11793615463902127No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15750.11763739300599017No Hit
GTCTGAGACGTGAGGACACGGCCTTATATTATTGTGCAAAAGCTCGCCGA15720.11741332178121687No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15720.11741332178121687No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15570.11629296565735028No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC15470.11554606157477257No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15450.11539668075825703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15240.11382818218484382No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15210.1136041109600705No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA15170.11330534932703941No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15160.11323065891878165No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15150.11315596851052387No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14960.11173685075362623No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14850.11091525626279074No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14670.10957082891415085No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14440.10785294952422211No Hit
GCCCTGAGATGCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAGA14090.1052387852352001No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13970.10434250033610683No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13920.10396904829481797No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13670.1021017880883737No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13660.10202709768011592No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13410.10015983747367163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)