FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338860
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTGGATACGTCACCTCTGACGTTCGGTCAAGGGA56000.4182662862435206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49070.36650583332088493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43480.32475389510479064No Hit
CAGTAATACACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA37460.2797902693336122No Hit
GTGTTAGTAGCGTCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTCCCAGGCTCCTCATCTATGCTGCAT35510.26522563972334673No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32720.24438701581942848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29250.21846944415398173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28570.21339049639245328No Hit
GTGTATTACTGTCAGCAGTTGGATACGTCACCTCTGACGTTCGGTCAAGGGACCAAGGTGGAGATCAAACGAA28350.21174730741078232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25650.19158089718118398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24980.1865766398279133No Hit
GCCTTATATTATTGTGCAAAAGCTCGCCGATTTTACAGCTCGTCCTTGTT24360.18194583451593146No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24110.18007857430948718No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATT22690.1694725363368836No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21820.1629744708184575No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21570.16110721061201322No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCGACTGGTGGAGT21370.15961340244685776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21270.15886649836428005No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCGTCTACTTAGCCTGGTACC21070.15737269019912464No Hit
GTAATACACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20900.15610295325874252No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT20670.15438507386881375No Hit
ATATAAGGCCGTGTCCTCACGTCTCAGACTGTTCATTTCCAGCTGCAGGG20400.15236843284585394No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19100.14265867977234364No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19100.14265867977234364No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19000.14191177568976593No Hit
CTACTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA18770.14019389629983717No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG18670.13944699221725945No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18520.13832663609339288No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18450.1378038032355885No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17580.13130573771716236No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGACCGAACGTC17470.13048414322632687No Hit
CTCCAGGCCCTTCCCTGGGGCTTGCCGGACCCAATGGAAGGCATAACCTT17390.1298866199602647No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17230.12869157342814036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17000.12697369403821163No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16960.12667493240518052No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16750.1251064338317673No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16650.12435952974918961No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16580.1238366968913852No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16370.12226819831797202No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16330.12196943668494092No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16320.12189474627668315No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15930.11898182035463005No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15840.1183096066803101No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGACGATTTTGCAGTGTATTACTGTCAGCAGTTGGATACGTCA15490.11569544239128811No Hit
GCATAGATGAGGAGCCTGGGAGGCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGACGCTACTAACACTCTGA15470.11554606157477257No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15400.11502322871696817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15310.11435101504264822No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15270.11405225340961712No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15180.11338003973529719No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15110.1128572068774928No Hit
GTCTGAGACGTGAGGACACGGCCTTATATTATTGTGCAAAAGCTCGCCGA14970.11181154116188399No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC14910.11136339871233736No Hit
GCCCTGAGATGCCCAGGTGTTTCCATTCAGTGATCAGCACTGAACACAGA14700.10979490013892415No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14510.1083757823820265No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14450.10792763993247988No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14330.10703135503338662No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14190.10598568931777781No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCA14120.10546285645997341No Hit
GTCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC13850.10344621543701357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)