FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462813
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45070.3081050004340951No Hit
GTATTACTGTCAGTTGTATGGTACCTCACCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA43450.29703044750080837No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41160.28137567823091536No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37390.2556034161577727No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33330.22784867238669604No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT32990.22552438349946305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32510.2222430344821929No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC31700.21670575801554948No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28880.19742783253908736No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27710.18942954430949135No Hit
CAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26650.1821832318963531No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26040.17801318418690565No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25650.17534708811037364No Hit
GTGTATTACTGTCAGTTGTATGGTACCTCACCTTTGTACACTTTTGGCCA25640.17527872667251385No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24110.16481942667996524No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24000.1640674508635075No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACCA23540.16092282472195693No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT23060.15764147570468678No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT23040.1575047528289672No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22960.15695786132608885No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAACCTGAAGATT22270.152240922113763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21790.14895957309649285No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20740.1417816221212144No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20320.138910441731103No Hit
GTATGGTACCTCACCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19940.13631270709243082No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19640.13426186395663697No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19470.13309971951302046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19450.13296299663730088No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19400.1326211894480019No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18990.1298183704957503No Hit
GTACCTCACCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA18900.12920311755501215No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGACCTGCT18560.12687882866777914No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC18270.12489634696984508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18070.1235291182126492No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTGGCCTGGTACC17980.12291386527191103No Hit
GTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17900.12236697376903267No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17810.12175172082829454No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG17400.11894890187604294No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC17140.11717150449168827No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17060.11662461298880991No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC16740.1144370469772965No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG16560.11320654109582018No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16510.11286473390652121No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16300.1114291437114655No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15500.10596022868268193No Hit
GTACCATACAACTGACAGTAATACACTGCAAAATCTTCAGGTTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC15310.10466136136334583No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14860.10158509665965507No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14710.10055967509175814No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAAGGTGAGGTACCATACAA14700.10049131365389835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.044.5063821
GTGGTAT5400.039.3139721
TGGTATC5700.037.2333452
GTATCAA55400.036.2523881
TATCAAC62050.032.085742
TCAACGC63600.031.3524554
ATCAACG63800.031.3069633
GAGTCGC652.6713815E-531.1995226
CAACGCA64500.030.9693185
AACGCAG64650.030.9463926
TTATGCG553.7490053E-430.6206424
CGCAGAG68300.029.1446538
ATAGCGG1402.3464963E-1028.7730963
ACGAACC1502.5465852E-1128.34636952
GCAGAGT70250.028.2368669
AGAGTAC70250.028.23107111
TACGTAT1654.8988422E-628.04301870
ACGCAGA72050.027.8614947
AATGGTA6450.027.67035370
CAGAGTA74050.026.8305710
Produced by FastQC (version 0.11.5)