FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551597
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16360.29659334622922173No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA11820.21428687973284846No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11780.2135617126271535No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10700.1939822007733907No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9950.180385317541611No Hit
GTATATTACTGTGCGAAAGGTTCACCTCTTAGTGGGAGCTACTCGGGATT9390.17023297806188215No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9190.16660714253340753No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA7370.13361203922428877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7360.133430747447865No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7240.13125524613078027No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6770.12273453263886497No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6650.12055903132178021No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT6630.12019644776893276No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6600.11965257243966156No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6590.11947128066323784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6500.11783965467542427No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCGGGAACGTTCGGCCA6500.11783965467542427No Hit
GTATGGTAGCTCACCTCCGGGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6410.11620802868761071No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6310.11439511092337341No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6090.11040669184205136No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT6080.11022540006562763No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT5850.10605568920788185No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5690.10315502078510218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC200.00853909449.8356173
TAGACCA359.833836E-442.03384860
ACCCTGG4450.032.62360470
GTATCAA32700.032.2369651
AACTCGG450.003994635331.59233948
CTGTTTA900.004573715430.72487470
AACTGTA1154.1710588E-430.0569470
TATCAAC34550.029.7327292
CGGACCT450.00555826529.5295375
CTTACCC500.0056561529.42369560
ATCAACG34800.029.3094523
TCAACGC34750.029.2533634
AACGCAG34850.029.172076
CGCAGAG34400.029.1673568
GGTATCA13000.029.1611161
CAACGCA35200.028.973765
CGCGGTA500.006278371428.80196853
GCAGAGT34550.028.7522439
AGAGTAC35050.028.53424811
CTCGCTA1650.028.25904323
Produced by FastQC (version 0.11.5)