FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609722
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT15670.25700237157261835No Hit
GAACAGGGGACTGGCGTGGCTTCTGCTGATACCAGGAAGCAAATTTATCC9000.14760825425357785No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8930.14646019005382782No Hit
CCTATGAGCTGACTCAGCCACCCTCACTGTCCGTGTCCCCAGGACAGACA8520.13973581402672036No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8490.13924378651254177No Hit
CCCCTGTTCTGGTCATCTATCAAGATAACAAGCGGCCCTCAGGGATCCCT8210.13465152971354158No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC8130.13333945634239866No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7370.12087475931654099No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGTGCTCCTGTCCCACGTCTGACAGAAATAGTCAG7360.12071075014514812No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7010.11497042914639787No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6980.11447840163221927No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT6860.1125102915755049No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6640.10890208980486189No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT6540.10726199809093324No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6430.10545789720561174No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6310.10348978714889738No Hit
GAGTGGCTGTGACCCCAGCGTTGGAGCCAGAGAATCGCTCAGGGATCCCT6290.10316176880611164No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6200.10168568626357587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3500.084.0494170
TACGTAT852.0569132E-856.3395570
GCGTTAC200.008919849.28918515
CGCGTTA200.00892269149.2851414
TCTCGTA3200.049.268138
GACCGGA606.57508E-445.60820870
CGTATGC4650.044.8881841
TCGTATG4500.044.59049240
GCCGTCT4500.044.06509446
CCGTCTT4800.042.2653947
CTCTTAT1400.042.258271
ATCTCGT3800.042.1887437
GTATGCC5000.041.23644342
GCGATCT3850.041.1815134
TGCTTGA5200.041.13109255
GATCTCG3950.040.44201736
ACGAGAC5450.040.412622
ATGCCGT5050.040.40401544
CTTGAAA5450.040.26566357
CCCACGA5600.039.9040419
Produced by FastQC (version 0.11.5)