FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609722
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40420.6629250707699574No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9050.14842830011054217No Hit
CCCCTGTTCTGGTCATCTATCAAGATAACAAGCGGCCCTCAGGGATCCCT8490.13924378651254177No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC8310.13629162142747023No Hit
GAACAGGGGACTGGCGTGGCTTCTGCTGATACCAGGAAGCAAATTTATCC8270.13563558474189877No Hit
CCTATGAGCTGACTCAGCCACCCTCACTGTCCGTGTCCCCAGGACAGACA8240.13514355722772017No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7680.12595904362971977No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACGGTGCTCCTGTCCCACGTCTGACAGAAATAGTCAG7470.12251485103046962No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7350.12054674097375524No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC7150.11726655754589797No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6820.11185425488993346No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC6660.10923010814764761No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6570.10775402560511184No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6530.10709798891954038No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6500.10660596140536178No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6470.1061139338911832No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6420.10529388803421887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6380.10463785134864742No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA200.008852554549.383661
GTATCAA37050.033.766611
TAAACGC400.003276455732.898134
AATGGTA5250.032.7195970
TCGCTAT1450.031.8237724
CTCGCTA1500.030.74023423
GTAAACT450.005830229729.242784
GCATCTA450.00583256829.2403833
ATGGCAA4000.029.20223470
ACGGGTA1252.1645974E-1028.95748116
TATCAAC43400.028.7313692
ATCAACG43350.028.5321373
AACGCAG44000.028.486796
CGCAGAG44150.028.166468
TCAACGC44050.028.1557184
CAACGCA44550.028.0635595
CAGAGTA44900.027.54943810
AGAGTAC44800.027.5329811
GCAGAGT45150.027.4697559
ACGCAGA46750.026.9518367
Produced by FastQC (version 0.11.5)