FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203108
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49800.41392792666992484No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45210.37577673824793784No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36130.3003055419796061No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35080.29157814593536074No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33270.2765337775162329No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30110.2502684713259325No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29550.24561386010233496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28260.23489163067654775No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27430.22799283189871566No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27040.22475122765371025No Hit
GATCTATGCTGTATCCACTTTGAAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGCGTCTGGGACAGACTTCA26460.2199303803149842No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25790.21436147045818No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25400.21111986621317455No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23330.19391442829737648No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22500.18701562951954437No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21860.1816960738354329No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21450.17828823347529899No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACACGCTGACAGTTTC21390.17778952512991353No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21380.17770640707234928No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21030.17479727505760081No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20850.17330115002144444No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGCTTCCACTGATACCAGGC20540.17072449023695294No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAGTG20490.17030889994913176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19340.16075032332924394No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCAGGGCCGAAAGTGAATGGGAAA19290.16033473304142273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19180.15942043440821604No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19010.15800742742962395No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACACG18940.15742560102667424No Hit
GTTCAGCGGCAGTGCGTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA18250.15169045505474155No Hit
ATGCTGTATCCACTTTGAAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGCGTCTGGGACAGACTTCACTCTCA18140.15077615642153488No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17680.14695272577357976No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGCTCCAGATGCGACAT17640.14662025354332278No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17100.14213187843485373No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16920.14063575339869738No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16540.1374772672112562No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16360.13598114217509982No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16290.13539931577215014No Hit
GTAATACATGGCCGTGTCTGCGGCCGTCACAGAGGTCAGTTTCAGGGAGA15650.13007976008803865No Hit
AGTCAGGATATTGGCAACTGGTTAGCCTGGTATCAGTGGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATG15490.12874987116701078No Hit
GGATATTGGCAACTGGTTAGCCTGGTATCAGTGGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGTATC15050.1250926766341841No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14820.12318096131020656No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14650.12176795433161446No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14470.1202718292954581No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14440.12002247512276536No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14420.11985623900763688No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14230.11827699591391629No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGCTTCCACTGATACCAGGCTAACC14110.1172795792231454No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14020.1165315167050672No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13880.11536786389916781No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTGGCAACTGGTTAGCCTGGTATCAGTGGAAG13740.11420421109326842No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13640.11337303051762603No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13530.11245873188441935No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13510.11229249576929087No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13380.11121196102095574No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13300.11054701656044179No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13280.11038078044531331No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13270.11029766238774906No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13210.10979895404236362No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13100.10888465540915696No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12870.10697294008517938No Hit
GAGTGAAGTCTGTCCCAGACGCACTGCCGCTGAACCGTGATGGGACCCCA12830.10664046785492241No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12750.1059755233944085No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG12510.10398069001286668No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACACGCTGACAGTTTCCCATTCACTTT12370.10281703720696729No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12350.10265080109183881No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12340.10256768303427456No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGG12330.10248456497671031No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12230.10165338440106791No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGCTCCAGATGCGACATCC12120.10073908576786123No Hit
GGTTAGGGGCTTTCCCTGGCTTCCACTGATACCAGGCTAACCAGTTGCCA12080.10040661353760427No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12070.10032349548004003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT150.002618227467.1845620
CTAGCGT150.0026212567.16501617
ACGCGAT200.005529824655.627163
TCGCGTT252.6627086E-454.79335438
CTACGCT3900.042.3518528
ACTACGC3850.041.97728327
TACGCTG4200.040.16952529
GTATCAA33250.039.098391
CGAGACT4150.038.87150223
TCGTATG1950.038.858740
ATCTCCG4150.038.83757810
ACGAGAC4200.038.40395722
TCTCCGA4400.037.3940411
ACGCTGC4350.037.27190830
CCCACGA4350.037.058119
CGGGACT1550.036.83242817
AACGGAT7100.035.8818570
GAGACTA4500.035.8526424
TATACAC4600.035.768213
GTGGTAT3400.035.5683021
Produced by FastQC (version 0.11.5)