FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971786
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58210.5990001914001643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57630.5930317991821245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40620.4179932618909925No Hit
GCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCGCCATCAGCAGT37550.3864019444610233No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37210.3829032317814827No Hit
CTCCAGAGCAGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT36860.37930161578783805No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36530.3759058064224016No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35570.36602708826840474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34940.3595441794798443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32520.33464157746664386No Hit
GTGTAAGGGGTGCCGAAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTC31960.3288789918768124No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31950.32877608856270824No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31760.3268209255947297No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30650.3153986577291708No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29410.3026386467802582No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27630.2843218568697224No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27470.2826754038440562No Hit
AGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAA27000.2778389480811619No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26180.2694008763246229No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26060.2681660365553733No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25370.2610657078821881No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24190.24892311681790025No Hit
GTCTCCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGATATTGCCAC23990.24686505053581756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23330.24007343180494473No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22740.2340021362728008No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22680.233384716388176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22570.2322527799330305No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21480.22103631869567994No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21310.21928696235590964No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20680.2128040535673492No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20160.20745308123393422No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20090.20673275803520527No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20020.20601243483647633No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19610.20179339895820686No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19340.1990150094773952No Hit
CATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTG19230.19788307302224975No Hit
GATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTTCGGCACC19110.19664823325300015No Hit
GGCCAAAAGTGTAAGGGGTGCCGAAACTCTGCTGACAGTAGTAAGTTGCA18620.19160597086189757No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18550.19088564766316865No Hit
GTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAA18180.1870782250413157No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG17430.17936047648350562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17420.1792575731694015No Hit
ACTTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17250.17750821682963122No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17210.17709660357321466No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17190.17689079694500642No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16920.1741124074641948No Hit
ACCTAGATGGGACCCCACTCTCCAGAGCAGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTGCTG16910.17400950415009064No Hit
GGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCAT16590.1707165980987584No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16520.16999627490002941No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16510.1698933715859253No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16490.16968756495771703No Hit
ATCCTGACTTGCCCGGCAAGTAATGGTGACTCTGTCTCCCACCGATGCAGACAGGGAGGATGGAGACTGGGTCAT16470.16948175832950876No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATCCACTGCCA16250.1672178854192178No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16240.1671149821051137No Hit
GGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATT16170.16639465890638472No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16150.16618885227817648No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15970.16433659262430206No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15970.16433659262430206No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15590.16042626668834498No Hit
CCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC15580.16032336337424083No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15570.16022046006013668No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGAAACCAATTTAAATAGGTGGCA15430.15877981366267882No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15430.15877981366267882No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15340.1578536838357416No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15290.15733916726522096No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15250.15692755400880443No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15210.15651594075238787No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATCCACTGCCA14940.15373755127157626No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14660.15085625847666048No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14630.1505475485343481No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14430.1484894822522654No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14330.14746044911122408No Hit
CCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTT13870.1427268966624339No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13610.14005141049572642No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGA13600.1399485071816223No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13330.13717011770081067No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT13190.1357294713033528No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13080.13459753484820733No Hit
CACCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAAAGCCC12720.1308930155404585No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC12710.13079011222635437No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT12580.12945236914300062No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12480.12842333600195927No Hit
GCCCCTAACCTCCTGATCTATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGAT12430.12790881943143861No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12410.12770301280323035No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12400.1276001094891262No Hit
TTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGA12350.12708559291860552No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12330.12687978629039728No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12140.12492462332241872No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11970.12317526698264844No Hit
CCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCG11950.12296946035444016No Hit
GTCTCCCACCGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT11920.12266075041212778No Hit
GATCTATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11840.1218375238992947No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11650.11988236093131614No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11440.11772139133512934No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11360.11689816482229626No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11360.11689816482229626No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTA11140.11463429191200532No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10950.11267912894402678No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10930.1124733223158185No Hit
GTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGG10690.11000364277731929No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10680.10990073946321516No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10550.10856299637986142No Hit
CCCCTAACCTCCTGATCTATGATGCATCTGCTCTGGAGAGTGGGGTCCCA10510.10815138312344488No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA10500.10804847980934074No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGAAACCAATT10490.1079455764952366No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10410.10712234998240353No Hit
ATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT10270.10568170358494565No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTAGATGGGACCCCA10200.10496138038621672No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT10160.10454976712980019No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC10110.10403525055927951No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG10030.10321202404644644No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGAAACCAATTTAAAT9820.10105105445025962No Hit
ATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCG9770.10053653787973896No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG9750.10033073125153069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)