FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971786
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58480.6017785808809758No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44320.4560674881095221No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41890.43106198278221747No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41340.4254023005064901No Hit
CTCCAGAGCAGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTT37990.39092969028160524No Hit
GCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCGCCATCAGCAGT34860.35872095296701123No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33280.3424622293385581No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32400.3334067376973943No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32030.3295993150755413No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30800.3169422074407328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30380.3126202682483592No Hit
GTGTAAGGGGTGCCGAAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTC29880.3074751025431525No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29880.3074751025431525No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28050.288643796062096No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27980.28792347286336706No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27040.2782505613375784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26900.2768099149401206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25550.2629179675360625No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25450.26188893439502114No Hit
AGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAA24970.25694957531802265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24200.24902602013200437No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24080.24779118036275474No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23940.24635053396529688No Hit
GTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAA23640.24326343454217284No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG23060.2372950423241331No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23030.23698633238182068No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22910.23575149261257106No Hit
GTCTCCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGGATATTGCCAC22620.23276729650355119No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22550.23204697330482227No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22230.22875406725348996No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22090.2273134208560321No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20100.20683566134930945No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19350.19911791279149937No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18830.1937669404580844No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18620.19160597086189757No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18370.18903338800929423No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18330.1886217747528777No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18240.18769564492594049No Hit
GATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTTCGGCACC17990.18512306207333712No Hit
ACTTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA17620.18131563945148418No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17580.18090402619506765No Hit
CATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATTTTG17490.17997789636813044No Hit
CCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG16750.17236305112442452No Hit
GGCCAAAAGTGTAAGGGGTGCCGAAACTCTGCTGACAGTAGTAAGTTGCA16700.17184853455390384No Hit
ATCCTGACTTGCCCGGCAAGTAATGGTGACTCTGTCTCCCACCGATGCAGACAGGGAGGATGGAGACTGGGTCAT16450.1692759517013005No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16430.1690701450730922No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16350.16824691856025914No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16260.16732078873332193No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16260.16732078873332193No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16130.16598304564996819No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15940.16402788268198967No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15610.16063207331655321No Hit
GGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCAT15540.1599117501178243No Hit
GGTTAGGGGCTTTCCCTGGTTGCTGCTGAAACCAATTTAAATAGGTGGCA15470.15919142691909535No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATCCACTGCCA15420.1586769103485747No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15410.15857400703447055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15370.15816239377805402No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15090.15528110098313824No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGCGAGAGTGAAATCTGTCCCAGATCCACTGCCA15070.15507529435493No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14910.15342884132926385No Hit
ACCTAGATGGGACCCCACTCTCCAGAGCAGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTGCTG14900.15332593801515973No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14810.15239980818822252No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14770.151988194931806No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14420.1483865789381613No Hit
CCCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC14240.14653431928428687No Hit
GGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATT14190.1460198027137662No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14090.14499076957272486No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13920.14324141323295458No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13590.13984560386751815No Hit
CCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTT13080.13459753484820733No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGA12610.129761079085313No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12510.12873204594427168No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12450.12811462605964688No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12440.12801172274554273No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12340.1269826896045014No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12270.12626236640577246No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12250.1260565597775642No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12220.12574784983525178No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12180.12533623657883525No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12110.12461591338010633No Hit
GCCCCTAACCTCCTGATCTATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGAT12080.1243072034377939No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11930.12276365372623191No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11860.12204333052750296No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC11810.12152881395698231No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11810.12152881395698231No Hit
CACCATTACTTGCCGGGCAAGTCAGGATATTGCCACCTATTTAAATTGGTTTCAGCAGCAACCAGGGAAAGCCC11780.12122010401466989No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11760.12101429738646163No Hit
CCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCG11670.12008816755952442No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11420.11751558470692108No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11310.11638364825177559No Hit
GATCTATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA11190.11514880848252598No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG11190.11514880848252598No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11160.1148400985402136No Hit
TTACTACTGTCAGCAGAGTTTCGGCACCCCTTACACTTTTGGCCAGGGGA11150.11473719522610945No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11120.11442848528379705No Hit
GTCTGCATCGGTGGGAGACAGAGTCACCATTACTTGCCGGGCAAGTCAGG11100.11422267865558877No Hit
GTCTCCCACCGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT11040.11360525877096399No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10860.11175299911708957No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTGCTGCTGAAACCAATT10830.11144428917477715No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCGGTGGGAGACAGAGTCACCATTA10570.10876880300806967No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10540.10846009306575727No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTAGATGGGACCCCA10430.1073281566106118No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT10370.106710736725987No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10130.10424105718748779No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9970.10259460416182163No Hit
ATCTGGGACAGATTTCACTCTCGCCATCAGCAGTCTGCAACCTGAAGATT9910.10197718427719683No Hit
ATGATGCATCTGCTCTGGAGAGTGGGGTCCCATCTAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCG9840.10125686107846789No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG9790.10074234450794724No Hit
GTCCCAGATCCACTGCCACTGAACCTAGATGGGACCCCACTCTCCAGAGC9730.10012492462332243No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9730.10012492462332243No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTA200.005855125454.82841560
TCGGATA200.00816533950.40608218
TACGTAT554.3495305E-449.60209370
TAGTGAT2100.048.7163470
GGTATCA10250.044.5592841
GTATCAA24300.037.729371
GTACGTA500.002086460436.07050769
GTGGTAT2900.034.741331
ATAGGTG18800.033.7386670
TATCAAC26950.033.6438562
TGGTATC3100.033.581562
ATCAACG27350.033.0171243
TCAACGC27700.032.4804234
CGGACGG1700.032.2520160
CAACGCA28100.032.139195
GATTCAC2100.031.9840811
AACGCAG28500.031.6881166
ACGCAGA29550.030.5621437
CGCAGAG29700.030.5208268
ATTGCCG1900.030.16531455
Produced by FastQC (version 0.11.5)