FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716451
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTCTT30160.42096389006366103No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25740.35927090617502105No Hit
GGCGTAGACTCTGTGTTGCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23530.328424414230701No Hit
CTCCCAGGAGAGTGCCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22800.3182353015070116No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCG21060.29394892323410815No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19140.2671501610019387No Hit
GTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17260.24090970631627284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGCTGGTGCAGCCACACTTC15850.22122936530202345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15730.21955444266251287No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15540.21690248181662108No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15150.2114589832382117No Hit
GTATTTTTGTCAACAATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTG14440.20154902428777405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14030.19582637193611288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14000.19540764127623525No Hit
CCAGTGGCCCTGGTGGAGGCATCGTAAATCAGAAGCCTGGGACTCTGACC12960.18089164506714348No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12550.17516899271548228No Hit
GGTATCAGCAGAGGCCAGGTCAGAGTCCCAGGCTTCTGATTTACGATGCCTCCACCAGGGCCACTGGTGTGCCGG12530.1748898389422305No Hit
ATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTGGGACCTCAGTGGCTCTCACAGGAAGTGTGGCTGCACCAG12280.17140041677658346No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12190.17014422479695052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12180.17000464791032463No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12060.16832972527081405No Hit
ATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTGGGACCTCAGTGGCT11920.1663756488580517No Hit
GAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGGCCGGCACACCA11800.16470072621854112No Hit
CTCTGGGATAGAAGTTCTTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11600.16190918848602345No Hit
GTGTCTGGTGGCCCCATGAAGAGAGGAGACGATTGGGCCTGGATCCGGCA11490.16037384273313876No Hit
CAAAAATACTGTCCAACATCGTCAGACTGTAGGCCATTTATGGTGAGAGT11460.15995511207326113No Hit
GACTGTGATGACGCAGGTTCCAGCCACCCTGTCCGTGTCTGCTGGAGAAA11310.1578614587738729No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAGAACTT11190.15618653613436229No Hit
GAAATAGACAGCCGTGTCTTCGGGGGTCGAAGATGTCAAAGTCAGGGTGA11170.15590738236111054No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11080.1546511903814776No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTCTTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11070.1545116134948517No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11000.15353457528847053No Hit
GTCCCAGACCCACTTCCAATAAACCTGGCCGGCACACCAGTGGCCCTGGT10990.15339499840184465No Hit
GGCCAGGTCAGAGTCCCAGGCTTCTGATTTACGATGCCTCCACCAGGGCC10820.15102219132920466No Hit
GACTTCACTCTCACCATAAATGGCCTACAGTCTGACGATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGG10660.14878896114319054No Hit
CACAGGAAGTGTGGCTGCACCAGCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT10520.1468348847304282No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10210.14250800124502583No Hit
GGAGAAAGAGTGTCCCTCTCCTGCAGGGCCAGTGAGAATATTGATGATAA10110.141112232378767No Hit
GTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9750.13608746446023523No Hit
GTCTGGGACAGACTTCACTCTCACCATAAATGGCCTACAGTCTGACGATG9670.1349708493672282No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9660.1348312724806023No Hit
GTCCAACATCGTCAGACTGTAGGCCATTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGG9570.13357508050096936No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9570.13357508050096936No Hit
GTCCGTGTCTGCTGGAGAAAGAGTGTCCCTCTCCTGCAGGGCCAGTGAGA9540.1331563498410917No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9330.1302252352219482No Hit
GATGAAGACAGCTGGTGCAGCCACACTTCCTGTGAGAGCCACTGAGGTCCCAGGGCCGAAAGTGAAGGGAGGCCA9240.12896904324231523No Hit
GTCTATTTCTGTGCCAGGCACGACCTTTTAACCATTCCTCAAATGCGGGG9230.12882946635568937No Hit
GGCCACTGGTGTGCCGGCCAGGTTTATTGGAAGTGGGTCTGGGACAGACT9050.12631708239642347No Hit
GGTTTATTGGAAGTGGGTCTGGGACAGACTTCACTCTCACCATAAATGGC9050.12631708239642347No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8790.12268808334415053No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8560.11947781495175525No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8450.11794246919887054No Hit
TCGTCAGACTGTAGGCCATTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGGCCGGCACA8320.11612796967273409No Hit
GCTGATACCAGGCCAATTTATCATCAATATTCTCACTGGCCCTGCAGGAGAGGGACACTCTTTCTCCAGCAGACA8210.11459262391984937No Hit
CCTCTGTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGTA8120.11333643194021642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8110.11319685505359053No Hit
GTGTTGCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCT8040.11221981684720937No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8030.11208023996058349No Hit
GACCTGGCCTCTGCTGATACCAGGCCAATTTATCATCAATATTCTCACTGGCCCTGCAGGAGAGGGACACTCTTT8010.11180108618733173No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7960.1111032017542023No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7950.11096362486757642No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7950.11096362486757642No Hit
CTCCTGCAGGGCCAGTGAGAATATTGATGATAAATTGGCCTGGTATCAGC7750.10817208713505878No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7750.10817208713505878No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7740.1080325102484329No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7700.10747420270192938No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTCTGTGTTGCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7690.1073346258153035No Hit
ACCAGACACCGTGCATGTGAGGGACAGGGTCTCCGAAGGTCTCACCAGTC7660.10691589515542585No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7660.10691589515542585No Hit
CTCACAGGAAGTGTGGCTGCACCAGCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGC7620.1063575876089223No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7590.10593885694904466No Hit
GTCCTTGCTGTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7490.10454308808278583No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG7480.10440351119615995No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAGAACTTCTATC7470.10426393430953407No Hit
CTGCTACTCTGGCTCCCAGATGCCGCTGGACGGACTGTGATGACGCAGGTTCCAGCCACCCTGTCCGTGTCTG7450.10398478053628231No Hit
GTCTGACGATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGGCCTC7430.10370562676303055No Hit
GTTAAAAGGTCGTGCCTGGCACAGAAATAGACAGCCGTGTCTTCGGGGGTCGAAGATGTCAAAGTCAGGGTGAA7370.10286816544327526No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7280.10161197346364231No Hit
GCCTCTGTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7280.10161197346364231No Hit
CAATAAACCTGGCCGGCACACCAGTGGCCCTGGTGGAGGCATCGTAAATCAGAAGCCTGGGACTCTGACCTGGC7230.10091408903051291No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7220.10077451214388702No Hit
TTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGGC7220.10077451214388702No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7220.10077451214388702No Hit
CACCATAAATGGCCTACAGTCTGACGATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGGCCTCCCTTCA7190.10035578148400938No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGC7170.10007662771075762No Hit
ACACAGAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCG7170.10007662771075762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)