FastQCFastQC Report
Wed 8 May 2024
EGAF00004691836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716451
Total Bases52.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33290.46465145557756216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28830.40240016414241864No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27290.3809053236020328No Hit
GGCGTAGACTCTGTGTTGCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27170.37923040096252225No Hit
CTCCCAGGAGAGTGCCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26440.3690412882388328No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC22670.3164208019808752No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21500.3000903062456469No Hit
GTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20720.28920330908882813No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20130.28096827277790104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17980.25095924215333637No Hit
GGTTTATTGGAAGTGGGTCTGGGACAGACTTCACTCTCACCATAAATGGC17530.24467828225517166No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16810.23462874641810816No Hit
GTATTTTTGTCAACAATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTG16690.23295382377859758No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16630.2321163624588423No Hit
GTGTCTGGTGGCCCCATGAAGAGAGGAGACGATTGGGCCTGGATCCGGCA15560.21718163558987286No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14020.195686795049487No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13810.19275568043034347No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13760.19205779599721406No Hit
ATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTGGGACCTCAGTGGCTC13580.18954541203794814No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13570.18940583515132228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC13440.18759133562518582No Hit
CCAGTGGCCCTGGTGGAGGCATCGTAAATCAGAAGCCTGGGACTCTGACC13140.18340402902640934No Hit
GAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGGCCGGCACACCA13140.18340402902640934No Hit
GACTTCACTCTCACCATAAATGGCCTACAGTCTGACGATGTTGGACAGTA13110.1829852983665317No Hit
ATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGGCCTCCCTTCACT12970.18103122195376936No Hit
CTCTGGGATAGAAGTTCTTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12820.17893756865438112No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12760.17810010733462583No Hit
GGTATCAGCAGAGGCCAGGTCAGAGTCCCAGGCTTCTGATTTACGATGCC12750.17796053044799992No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTCTTCAGCAGGCACACAA12700.17726264601487052No Hit
GACTGTGATGACGCAGGTTCCAGCCACCCTGTCCGTGTCTGCTGGAGAAA12660.176704338468367No Hit
GTCCCAGACCCACTTCCAATAAACCTGGCCGGCACACCAGTGGCCCTGGT12550.17516899271548228No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12240.17084210923007992No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12010.16763184083768465No Hit
GAAATAGACAGCCGTGTCTTCGGGGGTCGAAGATGTCAAAGTCAGGGTGA11970.16707353329118113No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGCAACACAGAGTCTACGCCTGC11920.1663756488580517No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11870.1656777644249223No Hit
CAAAAATACTGTCCAACATCGTCAGACTGTAGGCCATTTATGGTGAGAGT11670.16288622669240466No Hit
GTCTGGGACAGACTTCACTCTCACCATAAATGGCCTACAGTCTGACGATG11270.15730315122736935No Hit
GGAGAAAGAGTGTCCCTCTCCTGCAGGGCCAGTGAGAATATTGATGATAA11240.1568844205674917No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT11240.1568844205674917No Hit
GGCCAGGTCAGAGTCCCAGGCTTCTGATTTACGATGCCTCCACCAGGGCC11170.15590738236111054No Hit
GTCCAACATCGTCAGACTGTAGGCCATTTATGGTGAGAGTGAAGTCTGTC10960.15297626774196701No Hit
GTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10800.1507430375559529No Hit
GTCCGTGTCTGCTGGAGAAAGAGTGTCCCTCTCCTGCAGGGCCAGTGAGA10680.1490681149164423No Hit
CTGCTACTCTGGCTCCCAGATGCCGCTGGACGGACTGTGATGACGCAGGT10650.14864938425656465No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGGCACTCTCCTGGGAG10630.14837023048331288No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10480.14627657718392467No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10480.14627657718392467No Hit
CCTCTGTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGTA10010.1397164635125082No Hit
TCGTCAGACTGTAGGCCATTTATGGTGAGAGTGAAGTCTGTCCCAGACCC10000.1395768866258823No Hit
GTCTATTTCTGTGCCAGGCACGACCTTTTAACCATTCCTCAAATGCGGGG9950.13887900219275287No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9740.13594788757360934No Hit
GGCCACTGGTGTGCCGGCCAGGTTTATTGGAAGTGGGTCTGGGACAGACT9720.1356687338003576No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9720.1356687338003576No Hit
GTGTTGCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGT9700.13538958002710583No Hit
GATGAAGACAGCTGGTGCAGCCACACTTCCTGTGAGAGCCACTGAGGTCC9560.13343550361434348No Hit
CACAGGAAGTGTGGCTGCACCAGCTGTCTTCATCTTCCCGCCATCTGATG9550.1332959267277176No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9510.13273761918121407No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9460.13203973474808464No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9310.12994608144869643No Hit
GCTGATACCAGGCCAATTTATCATCAATATTCTCACTGGCCCTGCAGGAG9300.12980650456207052No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGCAA9260.129248197015567No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9220.12868988946906348No Hit
CTCCTGCAGGGCCAGTGAGAATATTGATGATAAATTGGCCTGGTATCAGC9060.12645665928304936No Hit
CTTCCTGTGAGAGCCACTGAGGTCCCAGGGCCGAAAGTGAAGGGAGGCCA8960.12506089041679053No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC8890.12408385221040936No Hit
GCCTCTGTTGTGTGCCTGCTGAAGAACTTCTATCCCAGAGAGGCCAAAGT8860.12366512155053172No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8860.12366512155053172No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8840.12338596777727996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAGAACTTCTATC8740.12199019891102113No Hit
CTCTTTCTCCAGCAGACACGGACAGGGTGGCTGGAACCTGCGTCATCACA8640.1205944300447623No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8570.11961739183838113No Hit
CAATAAACCTGGCCGGCACACCAGTGGCCCTGGTGGAGGCATCGTAAATC8540.11919866117850349No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8490.11850077674537408No Hit
CTGGTGTGCCGGCCAGGTTTATTGGAAGTGGGTCTGGGACAGACTTCACT8480.1183611998587482No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGCCA8450.11794246919887054No Hit
ACAATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTGGGACCTCAGTGG8430.11766331542561878No Hit
GTCCTTGCTGTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGA8280.11556966212623054No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC8270.11543008523960466No Hit
CTGCTGATACCAGGCCAATTTATCATCAATATTCTCACTGGCCCTGCAGG8170.11403431637334585No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8160.11389473948671996No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8120.11333643194021642No Hit
ACCAGACACCGTGCATGTGAGGGACAGGGTCTCCGAAGGTCTCACCAGTC8120.11333643194021642No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC8100.11305727816696466No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8060.11249897062046113No Hit
GTCTGACGATGTTGGACAGTATTTTTGTCAACAATATAGATGGTGGCCTC8040.11221981684720937No Hit
ACACAGAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCG8020.11194066307395761No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7990.11152193241407997No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTCTGTGTTGCTCGTAGTCT7940.11082404798095054No Hit
TCTATATTGTTGACAAAAATACTGTCCAACATCGTCAGACTGTAGGCCAT7910.1104053173210729No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTCTTCAGCAGGCA7820.10914912534143997No Hit
GGTCAGAGTCCCAGGCTTCTGATTTACGATGCCTCCACCAGGGCCACTGG7790.1087303946815623No Hit
GTATCAGCAGAGGCCAGGTCAGAGTCCCAGGCTTCTGATTTACGATGCCT7770.10845124090831054No Hit
CCCTTCACTTTCGGCCCTGGGACCTCAGTGGCTCTCACAGGAAGTGTGGC7770.10845124090831054No Hit
GACCTGGCCTCTGCTGATACCAGGCCAATTTATCATCAATATTCTCACTG7660.10691589515542585No Hit
GCACACCAGTGGCCCTGGTGGAGGCATCGTAAATCAGAAGCCTGGGACTC7590.10593885694904466No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7590.10593885694904466No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7570.1056597031757929No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT7540.10524097251591526No Hit
CACCATAAATGGCCTACAGTCTGACGATGTTGGACAGTATTTTTGTCAAC7460.1041243574229082No Hit
GGACTGGTGAGACCTTCGGAGACCCTGTCCCTCACATGCACGGTGTCTGG7450.10398478053628231No Hit
TTTATGGTGAGAGTGAAGTCTGTCCCAGACCCACTTCCAATAAACCTGGC7450.10398478053628231No Hit
TTGCTGTCCTGCTCTGTGGCACTCTCCTGGGAGTTACCCGATTGGAGGGC7380.10300774232990115No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACC7340.10244943478339762No Hit
CAATATAGATGGTGGCCTCCCTTCACTTTCGGCCCTGGGACCTCAGTGGC7220.10077451214388702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)