FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38030.3904621683946389No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAG33390.34282229299755435No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAGCAGTAATAAT28770.29538776189097454No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27130.2785495300695912No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26530.27238920135445094No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25890.2658181840583014No Hit
GATTATTACTGCTGCTCATTTGCAGGCAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGT25880.26571551191304904No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACT25000.25668036313084336No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24940.2560643302593293No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23820.2445650499910675No Hit
TTATAACTCTGTCTCCTGGTACCAACACGTCCCAGGCAAAGCCCCCAAAC23430.24056083632622635No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22330.22926690034846928No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACTCTGTCTCCTGGTACCAACACGTCCCAGGCAAAGC21240.2180756365159645No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21160.2172542593539458No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21100.21663822648243175No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20070.20606299552144103No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19690.20216145400185223No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT19380.19897861749902973No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG18810.19312630521964652No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18660.19158622304086145No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18240.1872739929402633No Hit
GAGTAACTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC18150.18634994363299226No Hit
CAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA18060.18542589432572124No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17930.18409115643744084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17270.17731479485078658No Hit
GTACCAACACGTCCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCA17170.1762880733982632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17130.17587738481725385No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17100.17556936838149684No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16420.1685876625043379No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTG16390.16827964606858087No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16200.16632887530878648No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16170.16602085887302945No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15970.1639674159679827No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15920.16345405524172105No Hit
GAGTTATAATTACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGACTGACTGCCCAGGAGACCCGGACA15760.16181130091768364No Hit
CAGCTATACTATGCGGACTCTGTGGAGGGCCGATTCACCATTTCCAGAGACAACACCAAGAACTCAGTGTATCTG15750.16170862877243128No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGC15660.16078457946516025No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15530.15944984157687986No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14970.15370020144274898No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14520.1490799549063938No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14420.14805323345387045No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTTACGCTGTTCATTTGCAGATA14360.1474372005823564No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14180.14558910196781433No Hit
GTACTACTGTCTGCCCATCTCCCACAGTAATACACGGCCGTGTCCTCGGC14150.14528108553205732No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14130.14507574124155265No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14040.14415169193428162No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGC13760.14127687186721616No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC13680.14045549470519747No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13550.13912075681691707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13080.13429516599005722No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12950.13296042810177683No Hit
GTAATACACGGCCGTGTCCTCGGCTCTTACGCTGTTCATTTGCAGATACA12710.13049629661572074No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12430.1276214765486553No Hit
GTATAGCTGACTTCCATCTTTTTTTAAGTGGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT12080.1240279514648235No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCT12030.1235145907385618No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTGCA11980.12300123001230012No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGC11890.12207718070502908No Hit
GTTTGGGGGCTTTGCCTGGGACGTGTTGGTACCAGGAGACAGAGTTATAA11850.12166649212401973No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAAATATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG11740.12053709852624403No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11630.1194077049284683No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT11600.11909968849271131No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11590.11899701634745896No Hit
CTCCTGGGCAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT11520.11827831133069261No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11510.11817563918544026No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11480.11786762274968327No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11470.11776495060443092No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11450.11755960631392624No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11420.11725158987816924No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11300.11601952413514119No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGAC11300.11601952413514119No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11250.1155061634088795No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11170.11468478624686079No Hit
CCCCTGAGGGCCACTTAGTGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGACGTGTTGGTACCAGGAGA10930.1122206547608047No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10560.10842178538646823No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT10550.10831911324121588No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG10500.1078057525149542No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10490.10770308036970186No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATATTTGC10490.10770308036970186No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10460.10739506393394485No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10350.10626567033616913No Hit
GCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCATCCTCAG10310.10585498175515978No Hit
GTAGTACCTGGTACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAA10290.10564963746465511No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT10130.1040068831406177No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTC10120.10390421099536538No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10110.10380153885011303No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC10090.10359619455960836No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10060.10328817812385135No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10040.10308283383334668No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC9790.10051603020203824No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAA9760.10020801376628125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1500.058.6614670
TCGTATG650.058.33209640
CGTATGC750.055.2191841
ACTACGC1250.054.5496527
CTACGCT1300.052.46506528
AGACGAC200.00708616552.2462147
ACCGTAG200.00734856851.76798241
CATCTCG900.049.5107636
TATACAC1450.049.3413473
GGTATCA4050.048.805251
CGAGACT1400.048.6700423
CGACATT305.6547706E-447.0360654
CTTATAC1450.047.0062371
CACGAGA1450.046.99175621
ACGAGAC1450.046.99175622
ATCTCGT950.046.9582937
TCTCGTA900.045.81345738
TTATACA1500.045.425372
GTATGCC1150.042.0692942
ACACATC2000.040.882836
Produced by FastQC (version 0.11.5)