FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35930.36890101789164803No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAG31870.3272161269191991No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAGCAGTAATAAT28090.2884060560138156No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27600.28337512089645106No Hit
GTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACT27530.2826564158796847No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26450.27156782419243225No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25480.26160862610295554No Hit
GATTATTACTGCTGCTCATTTGCAGGCAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGT23290.23912342629269367No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22300.22895888391271224No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21650.22228519447131034No Hit
TTATAACTCTGTCTCCTGGTACCAACACGTCCCAGGCAAAGCCCCCAAAC21650.22228519447131034No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21140.2170489150634411No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20550.2109912584935532No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTAATTATAACTCTGTCTCCTGGTACCAACACGTCCCAGGCAAAGC20490.2103752256220392No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20350.20893781558850646No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20100.206371011957198No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG18530.19025148515258106No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18470.18963545228106704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18430.1892247637000577No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT18380.18871140297379602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17770.18244840211340344No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17620.1809083199346184No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGC17280.17741746699603891No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17250.17710945056028188No Hit
GAGTAACTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCATCCTCAGC16800.17248920402392673No Hit
CAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA16790.17238653187867437No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16620.17064110540938465No Hit
GTACCAACACGTCCCAGGCAAAGCCCCCAAACTCATCATTTATGATGTCA16270.16704758032555284No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTG16250.16684223603504816No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15910.16335138309646868No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15720.1614006123366743No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15540.15955251372213222No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15480.1589364808506182No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15440.15852579226960883No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15380.15790975939809482No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15210.15616433292880508No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15000.154008217878506No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14950.1534948571522443No Hit
GAGTTATAATTACCAACATCACTGCTGGTTCCAGTGCAGGAGATGGCGACTGACTGCCCAGGAGACCCGGACA14450.14836124988962743No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14260.14641047912983304No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14180.14558910196781433No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13780.14148221615772086No Hit
CAGCTATACTATGCGGACTCTGTGGAGGGCCGATTCACCATTTCCAGAGACAACACCAAGAACTCAGTGTATCTG13690.1405581668504498No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13550.13912075681691707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13440.13799136321914138No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12750.13090698519673008No Hit
GTACTACTGTCTGCCCATCTCCCACAGTAATACACGGCCGTGTCCTCGGC12680.13018828017996373No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12460.1279294929844123No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTTACGCTGTTCATTTGCAGATA12440.12772414869390764No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12200.12526001720785154No Hit
GTTTGGGGGCTTTGCCTGGGACGTGTTGGTACCAGGAGACAGAGTTATAA12070.12392527931957115No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGC12040.12361726288381415No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11970.12289855786704777No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAAATATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG11940.12259054143129078No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11940.12259054143129078No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGC11900.12217985285028143No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT11860.12176916426927208No Hit
GTATAGCTGACTTCCATCTTTTTTTAAGTGGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACT11750.12063977067149635No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11680.11992106565473001No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11380.11684090129715989No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11380.11684090129715989No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCTCCTGCA11340.11643021271615053No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11330.11632754057089818No Hit
CTCCTGGGCAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGATGTT11310.11612219628039351No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG11210.11509547482787014No Hit
GTAATACACGGCCGTGTCCTCGGCTCTTACGCTGTTCATTTGCAGATACA11200.11499280268261781No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT11020.11314470406807574No Hit
GGATGAGGCTGATTATTACTGCTGCTCATTTGCAGGCAGTTACTCTGTGATATTTGGCGGAGGGACCAAGCTGAC11020.11314470406807574No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC11000.11293935977757107No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10880.11170729403454302No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10750.11037255614626262No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC10740.1102698840010103No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT10730.11016721185575795No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGGCAGTCAGTCGCCATCT10600.10883247396747757No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10400.10677903106243083No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10390.10667635891717848No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10360.10636834248142148No Hit
GCTTGGTCCCTCCGCCAAATATCACAGAGTAACTGCCTGCAAATGAGCAGCAGTAATAATCAGCCTCATCCTCAG10150.10421222743112238No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10110.10380153885011303No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT9960.10226145667132798No Hit
GTAGTACCTGGTACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCCCCAA9940.10205611238082331No Hit
CCCCTGAGGGCCACTTAGTGACATCATAAATGATGAGTTTGGGGGCTTTGCCTGGGACGTGTTGGTACCAGGAGA9940.10205611238082331No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9930.10195344023557096No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATATTTGC9910.10174809594506629No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9800.10061870234729059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)