FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814609
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49370.6060576301022945No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACTGTTGCTGCCTGCATATGAGGCG40130.4926289790562098No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG28920.3550169467806027No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28820.35378936397707367No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27660.3395494034561366No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23240.28529024354015237No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23050.28295783621344717No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTC22360.2744875148690967No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20820.25558273969474926No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC19760.24257036197734128No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19300.23692348108110764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19200.23569589827757859No Hit
GATTATTATTGCGCCTCATATGCAGGCAGCAACAGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTCGGT18370.2255069610082874No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17400.21359940781405556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16780.20598839443217543No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCGCCTCATAT16740.20549736131076382No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16480.2023056460215883No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGTAGCAGTGACGTT15960.19592221544323718No Hit
GTCAGTCACCATCTCCTGCACTGGAAGTAGCAGTGACGTTGGTCGTTATAACTATGTCTCCTGGCACCAACACCA15820.1942035995182965No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15650.1921167087522971No Hit
GTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15650.1921167087522971No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15650.1921167087522971No Hit
CTCCTGCACTGGAAGTAGCAGTGACGTTGGTCGTTATAACTATGTCTCCTGGCACCAACACCACCCAGGCAAAGC15240.187083619257828No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15210.18671534441676926No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15100.18536500333288733No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15070.18499672849182858No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14890.18278707944547629No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14780.18143673836159432No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14600.17922708931524203No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14430.17714019854924265No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14000.1718615924940677No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13960.17137055937265608No Hit
CCTCAGGAGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG13640.1674422944013631No Hit
GGCCTCAGGAGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG13490.16560092019606953No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13430.16486437051395209No Hit
GTACTAAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC13300.16326851286936434No Hit
CTCCTGAGGCCCGCTTAGTGACGTCAAAAATTATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGGTGCCAGGAGA13300.16326851286936434No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13100.1608133472623062No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT13020.15983128101948296No Hit
TAGTTATAACGACCAACGTCACTGCTACTTCCAGTGCAGGAGATGGTGAC12860.1578671485338365No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12590.15455267496430802No Hit
ATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12580.15442991668395514No Hit
GTGTTGGTGCCAGGAGACATAGTTATAACGACCAACGTCACTGCTACTTCCAGTGCAGGAGATGGTGACTGA12570.15430715840360223No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT12530.1538161252821906No Hit
ATACCAGACTGTTGCTGCCTGCATATGAGGCGCAATAATAATCAGCCTCA12470.15307957560007315No Hit
TTATAACTATGTCTCCTGGCACCAACACCACCCAGGCAAAGCCCCCAAAC12460.15295681731972025No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12440.15271130075901446No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12250.15037889343230926No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12000.1473099364234866No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11970.14694166158242788No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC11940.1465733867413692No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11880.14583683705925174No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11820.14510028737713432No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC11140.13675272431313673No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGA10940.13429755870607862No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10860.13331549246325536No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG10830.13294721762219666No Hit
CCTGAGGCCCGCTTAGTGACGTCAAAAATTATGAGTTTGGGGGCTTTGCC10770.13221066794007924No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10650.13073756857584437No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA10620.13036929373478565No Hit
GACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACTGTTGCTGCCTGCATATGAGGCGCAATAATAAT10590.13000101889372695No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10460.12840516124913917No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10440.12815964468843336No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10350.1270548201652572No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10320.12668654532419849No Hit
GGAAGTAGCAGTGACGTTGGTCGTTATAACTATGTCTCCTGGCACCAACA10250.12582723736172813No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC10240.12570447908137522No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACTGTTG10220.12545896252066943No Hit
CATATGCAGGCAGCAACAGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCC10100.12398586315643456No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9990.12263552207255259No Hit
GCTTGGTCCCTCCGCCGAATACCAGACTGTTGCTGCCTGCATATGAGGCGCAATAATAATCAGCCTCATCTTCAG9920.12177621411008227No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT9910.12165345582972936No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA9910.12165345582972936No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9810.1204258730262003No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9700.11907553194231833No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATT9570.11747967429773057No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9350.11477899212996664No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTATTGCGCCTCATATGCAGGCAGCAACAGT9250.11355140932643758No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT9060.11121900199973238No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9040.11097348543902658No Hit
GTTGCTGCCTGCATATGAGGCGCAATAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGC8740.10729073702843941No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA8630.10594039594455744No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8590.10544936282314582No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC8580.10532660454279291No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8480.10409902173926387No Hit
GGACCAAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8390.10299419721608771No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG8280.10164385613220575No Hit
TCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8220.10090730645008833No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT8190.10053903160902962No Hit
CTATGTCTCCTGGCACCAACACCACCCAGGCAAAGCCCCCAAACTCATAA8160.1001707567679709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)