FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203523
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT5760.2830146961277104No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC5620.2761358667079396No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT5280.25943013811706783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.23682827002353543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.20636488259312213No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG4030.19801201829768625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.19653798342202108No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA3750.18425435945814478No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.1611611464060573No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3150.15477366194484163No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.14691214260796076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.13855927831252488No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2780.13659389847830467No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT2730.1341371736855294No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT2710.1331544837684193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.13266313880986425No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC2690.1321717938513092No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2690.1321717938513092No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC2680.13168044889275415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.13069775897564403No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC2630.12922372409997887No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC2530.12431027451442833No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA2520.12381892955587329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.11988816988743288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.11890547997032276No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2410.11841413501176772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2370.1164487551775475No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG2310.11350068542621719No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA2300.11300934046766214No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.11202665055055203No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG2260.11104396063344192No Hit
GATCTGGGACAGATCATTACACTCTCACCATCAACAGTCTACAACCTGAA2240.1100612707163318No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.10809589088211159No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC2180.1071132009650015No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC2120.10416513121367119No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT2090.10269109633800602No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA2090.10269109633800602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCA100.006992327145.6053570
TCGTAAT150.001345167679.43339560
TAGGTTT201.03735234E-466.2731932
GTAGGTT201.06282634E-465.9493331
GTTTAGA150.003344711163.13986621
GAATTAG150.003370902763.0157281
ATAAATC251.5958626E-460.7429152
CCTATAA251.6842673E-460.0845949
ACCTATA251.72617E-459.78688448
ATCGTAA200.00431316459.20820659
CCGCCTA200.004817725657.57731654
GCCGAGT303.330203E-452.33945558
TAAATCT303.831147E-450.875253
TACCTAT304.3526918E-449.57675647
CTTATAC900.049.012231
TATACAC900.049.0001873
CTCTACC304.726197E-448.75660744
TTTACTT300.00949917348.4892269
CATGAAT900.047.86397632
AAGGGGG1600.045.45864569
Produced by FastQC (version 0.11.5)