FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203523
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12090.5940360548930588No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC5760.2830146961277104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.2500945839045218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.23044078556231973No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4440.21815716159844342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.19702932838057616No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG3790.186219739292365No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA3740.18376301449958973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.17098804557715835No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT3440.16902266574293814No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA3200.15723038673761688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2920.14347272789807541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.14101600310530016No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC2820.13855927831252488No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.13659389847830467No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA2760.13561120856119455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.13069775897564403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.12922372409997887No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC2580.1267669993072036No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT2580.1267669993072036No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT2480.12185354972165308No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2470.12136220476309803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2430.1193968249288778No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG2400.11792279005321267No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG2370.1164487551775475No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC2350.1154660652604374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.11448337534332728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC2250.11055261567488688No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC2220.10907858079922171No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.10858723584066667No Hit
GTCTACAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACCTTTTCCCGCGCACTTTCGGCGGAGGGA2200.10809589088211159No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC2190.10760454592355655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2130.10465647617222623No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10367378625511614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10367378625511614No Hit
GTCCCAGATCCGCTGCCAGTGAACCTTGATGGGACCCCACTTTGTAAACT2100.10318244129656107No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG2090.10269109633800602No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG2070.10170840642089593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACG250.00106172584.28178470
CTTGTGA150.00323806863.6583910
CATGGAA150.00323806863.658393
ATGAAAC250.005007522657.0123369
TACAGTT908.957748E-539.01934470
TGTTAGA951.2315443E-436.96569470
TAGTTCT400.002832959933.8773235
CCAAATA602.0911131E-433.8012865
AGTGTTA802.0328944E-532.44313468
GAGTGTT802.748921E-531.04338567
ACCAGGT2509.822543E-1130.90331870
AGAGCAA950.00549534729.57255470
TACCACC654.008667E-529.3807968
GCACTTG450.005736041329.33061830
TCCAAAT550.00584412329.21565464
GTCCTGA450.006832275528.2995761
ATTAGGT450.00684048428.292625
TTGAGGC450.00684048428.292622
ATAAACG450.00684048428.292623
CGGGAGA805.1147817E-627.87109817
Produced by FastQC (version 0.11.5)