FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006131
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC23500.23356799462495442No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21090.20961485134639526No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT18350.18238181707948567No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18220.18108973881134763No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17740.17631898828283793No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17630.1752256912867211No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17140.1703555501222008No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17130.17025615948619016No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17130.17025615948619016No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17120.17015676885017955No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCC16590.16488906514161675No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16480.16379576814549995No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15170.15077559482810887No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT15000.14908595401592833No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15000.14908595401592833No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14570.14481215666747171No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCCGAGAGTATTA14540.14451398475943988No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14220.14133348440710006No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG13940.13855054659880275No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13820.13735785896667532No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13180.1309968582619957No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13090.13010234253790015No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12960.1288102642697621No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12930.12851209236173022No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTAGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC12910.128313311089709No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12750.12672306091353908No Hit
GATTATTACTGCACCTCATATACAGGCAGTAATACTCTCGGGGTGTTCGG12510.12433768564928424No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA11980.11906998194072144No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11910.11837424748864711No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTAGTTATAACTTTGTCTCCTGGTACCAACAACA11320.1125101999640206No Hit
GTATTACTGCCTGTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGC10950.10883274643162769No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10920.10853457452359584No Hit
GTAATACTCTCGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10900.1083357932515746No Hit
CAGTAATACTCTCGGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT10860.10793823070753211No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10790.10724249625545779No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10680.10614919925934097No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10660.10595041798731974No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC10560.10495651162721355No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10250.10187540191088436No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10220.10157723000285251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)