FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190085
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCT103340.868341336963326No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTGCTTATTGCTGGAACTGGATAAAACACT81130.6817160118815043No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80820.6791111559258373No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC75300.6327279143926694No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53800.4520685497254398No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53350.4482873072091489No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51750.4348428893734481No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47200.39661032615317393No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC43920.36904926958998724No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43290.3637555300671801No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42980.3611506741115131No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG41830.35148749879210306No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41740.3507312502888449No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39760.33409378321716515No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38970.3274556019107879No Hit
GTTTTATCCAGTTCCAGCAATAAGCACTACTTAGCTTGGTACCAGCAGAA38910.3269514362419491No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36140.3036757878638921No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC35890.3015750975770638No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35640.29947440729023556No Hit
GGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATT35560.2988021863984505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35430.2977098274492998No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTC35370.2972056617804611No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34150.2869542931807392No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33550.28191263649235143No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTT32460.2727536268417802No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATC31720.2665355835927686No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31030.2607376784011226No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGGC30440.25578004932420795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29700.2495620060751963No Hit
CTCCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAG29310.24628492922774423No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCC29290.24611687400479795No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT28770.24174743820819522No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28460.23914258225252819No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27730.2330085666149897No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26720.22452177785620356No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26580.2233453912955797No Hit
GTCAGGGACCCCGGAAACCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA26280.22082456295138583No Hit
CCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGC26270.22074053533991267No Hit
CTACACGGGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGG25910.21771554132688004No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25770.2165391547662562No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC25730.21620304432036366No Hit
GTTTATTATTGTCAGCAATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA25570.21485860253679356No Hit
GATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATTTTCTACATCT25540.21460651970237418No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24490.20578362049769555No Hit
GCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT24210.2034308473764479No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23350.19620447278975872No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23350.19620447278975872No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22940.19275934071936038No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22820.19175100938168282No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22710.19082670565547838No Hit
CCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTC22470.18881004298012327No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22320.1875496288080263No Hit
GTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCAATAAGCACTA22240.1868774079162413No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTGCTTATTGCTGGAACTG22070.18544893852119806No Hit
GGATAAAACACTCTGGCTGGACTTGCAGGTGATGGTGGCCCCCGCGCCCAGAGACACAGCCAGGGAGTCTGGAGA22000.18486074524088617No Hit
GCTAAGTAGTGCTTATTGCTGGAACTGGATAAAACACTCTGGCTGGACTT21870.18376838629173547No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21210.17822256393450886No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21060.17696214976241192No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20770.17452534902969116No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20460.17192049307402413No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATTTTCTACATCTCCGT20400.17141632740518534No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20330.17082813412487344No Hit
GGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGAT20180.16956771995277647No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20040.16839133339215268No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19880.16704689160858258No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19780.16620661549385127No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19690.16545036699059312No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19590.16461009087586181No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19510.16393786998407678No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19390.1629295386463992No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19220.161501069251356No Hit
TTATTATTGTCAGCAATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGA19220.161501069251356No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19100.16049273791367843No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA19000.15965246179894715No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18950.15923232374158147No Hit
GTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18710.15721566106622636No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18610.15637538495149506No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA18520.15561913644823688No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18460.15511497077939812No Hit
GGATCTCTGGTGCCTGCGGGGACAACGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGG18230.15318233571551612No Hit
CCCTGGTCCCTTGGCCGAACGTCCACGGAGATGTAGAAAATTGCTGACAATAATAAACTGCCACATCTTCAGC18050.15166983870899978No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17880.15024136931395657No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17830.14982123125659091No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17690.1486448446959671No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17630.1481406790271283No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA17190.1444434641223106No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17040.14318304995021364No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16980.14267888428137485No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT16970.14259485666990174No Hit
GCACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGC16890.1419226357781167No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16830.1414184701092779No Hit
CCGGAAACCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCC16820.14133444249780477No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16750.14074624921749285No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16700.14032611116012722No Hit
GCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC16580.13931777982244964No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC16460.13830944848477209No Hit
GCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCCACGG16150.13570459252910508No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16140.13562056491763194No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACAACGTGTTGA16120.13545250969468567No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15980.13427612313406187No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA15790.1326795985160724No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15520.13041085300629787No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15510.13032682539482474No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15480.13007474256040535No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15410.12948654928009345No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15250.12814210749652336No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15060.1265455828785339No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAG14560.12234420230487737No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14380.12083170529836103No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAA14220.11948726351479096No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14220.11948726351479096No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGC13790.11587407622144638No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG13660.11478171727229568No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13540.11377338593461811No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT13430.11284908220841369No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCTGA13400.11259699937399428No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC13310.11184075087073612No Hit
ACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCAATAA13180.11074839192158543No Hit
GTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCAATAAGCA13120.11024422625274664No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC13100.1100761710298004No Hit
GCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCTGACCGATT13020.10940395013801535No Hit
CCACTGAATCGGTCAGGGACCCCGGAAACCCGTGTAGATGCCCAGTAAAT12830.10780742552002588No Hit
GGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCA12820.10772339790855275No Hit
AAGCACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACT12770.10730325985118709No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12540.1053706247873051No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGAAACCCGTGT12470.10478243150699319No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATTTTCTACATCTCCGTGGACGTT12430.10444632106110067No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA12430.10444632106110067No Hit
GTCCACGGAGATGTAGAAAATTGCTGACAATAATAAACTGCCACATCTTC12410.1042782658381544No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12320.10352201733489624No Hit
ATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT12080.10150535465954112No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTC12070.10142132704806799No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11990.10074910615628296No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT11970.10058105093333669No Hit
AGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCAATAAGCACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGC11950.10041299571039042No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG11950.10041299571039042No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG11950.10041299571039042No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG11930.10024494048744417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)