FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190085
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCT94350.7928005142489822No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT80330.6749938029636539No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTGCTTATTGCTGGAACTGGATAAAACACT75800.6369292949663259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62270.523239936643181No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54520.45811853775150513No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53710.45131230122218163No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49380.4149283454543163No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45590.38308188070599997No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCAC40940.34400904137099453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39120.32871601608288487No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38960.32737157429931474No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG38510.32359033178302393No Hit
GTTTTATCCAGTTCCAGCAATAAGCACTACTTAGCTTGGTACCAGCAGAA38220.32115353105030314No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC38140.32048131015851805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38010.3193889512093674No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37330.31367507362919456No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37320.3135910460177214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36180.3040118983097846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36180.3040118983097846No Hit
GGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATT35230.29602927521983724No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34760.29207997748060016No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34330.2884667901872555No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC33080.2779633387531143No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32980.277123062638383No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTTTC30460.2559481045471542No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30210.2538474142603259No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29300.24620090161627112No Hit
GTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGGC28820.24216757626556087No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATC28430.2388904994181088No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCC26880.22586621963977366No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26330.2212447010087515No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACACGGGTT25880.2174634584924606No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25690.21586693387447115No Hit
GATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATTTTCTACATCT25690.21586693387447115No Hit
GTTTATTATTGTCAGCAATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA25670.2156988786515249No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25620.21527874059415925No Hit
GTCAGGGACCCCGGAAACCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTA24720.20771625556157752No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24560.20637181377800745No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24500.20586764810916866No Hit
CTCCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAG24300.20418709587970607No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGAAACCCGTGT24040.2020023779814047No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23980.20149821231256593No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT23920.2009940466437271No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGCTCATT23620.19847321829953324No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23570.19805308024216758No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC23010.19334753399967228No Hit
CTACACGGGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGG23000.19326350638819914No Hit
GGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATTTTCTACATCTCCGT22750.19116281610137092No Hit
GCTCATTTACTGGGCATCTACACGGGTTTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT21980.1846926900179399No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA21790.1830961653999504No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21780.1830121377884773No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG21650.1819197788393266No Hit
CCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTC21510.18074339227870279No Hit
ATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGAGAAATCTGTCCCAGACCCGCTGCCA21480.1804913094442834No Hit
CCCTGGCTGTGTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGC21220.178306591545982No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21120.17746631543125072No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21060.17696214976241192No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTGCTTATTGCTGGAACTG20660.17360104530348672No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20620.17326493485759423No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20620.17326493485759423No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20410.17150035501665847No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20170.16948369234130334No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20120.1690635542839377No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20020.1682232781692064No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19940.16755105727742137No Hit
GGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19830.16662675355121692No Hit
GGTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGAT19700.16553439460206623No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19690.16545036699059312No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19150.1609128759710441No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19140.16082884835957095No Hit
GTCTCTGGGCGCGGGGGCCACCATCACCTGCAAGTCCAGCCAGAGTGTTTTATCCAGTTCCAGCAATAAGCACTA19080.16032468269073216No Hit
GGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC18840.15830802001537705No Hit
GCTAAGTAGTGCTTATTGCTGGAACTGGATAAAACACTCTGGCTGGACTT18830.15822399240390395No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18800.15797190956948454No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA18690.1570476058432801No Hit
TTATTATTGTCAGCAATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGA18610.15637538495149506No Hit
GTCTGGGACAGATTTCTCTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18510.15553510883676375No Hit
CCCTGGTCCCTTGGCCGAACGTCCACGGAGATGTAGAAAATTGCTGACAATAATAAACTGCCACATCTTCAGC18250.1533503909384624No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18170.15267817004667733No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18090.1520059491548923No Hit
GGATAAAACACTCTGGCTGGACTTGCAGGTGATGGTGGCCCCCGCGCCCAGAGACACAGCCAGGGAGTCTGGAGA17780.14940109319922526No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17770.14931706558775215No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17650.14830873425007457No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17440.14654415440913884No Hit
GGATCTCTGGTGCCTGCGGGGACAACGTGTTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGCGGGGG17310.14545179545998815No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17290.14528374023704188No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17150.14410735367641808No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17070.14343513278463305No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16980.14267888428137485No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16970.14259485666990174No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16910.14209069100106295No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16700.14032611116012722No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACAACGTGTTGA16500.13864555893066463No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16340.13730111714709453No Hit
GCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTCCACGG15880.13343584701933053No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15810.13284765373901863No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15730.1321754328472336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15370.1291504388342009No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15270.12831016271946963No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15200.1277219694391577No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14800.1243608649802325No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14760.12402475453433999No Hit
CCGGAAACCCGTGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCC14500.1218400366360386No Hit
GCACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAACTGC14400.1209997605213073No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14200.11931920829184471No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14130.1187310150115328No Hit
GCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC14120.11864698740005966No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14060.11814282173122088No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA14000.1176386560623821No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13960.11730254561648959No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13770.11570602099850011No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13580.11410949638051063No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13400.11259699937399428No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGC13030.10948797774948849No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCTCTCTCACCATCAG12680.10654701134792893No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA12620.10604284567909016No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12570.1056227076217245No Hit
GTCCACGGAGATGTAGAAAATTGCTGACAATAATAAACTGCCACATCTTC12460.10469840389552007No Hit
GAGAGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12450.10461437628404695No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG12430.10444632106110067No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12410.1042782658381544No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC12120.10184146510543364No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12100.1016734098824874No Hit
ATTTTCTACATCTCCGTGGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT12060.10133729943659488No Hit
CCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAA12010.10091716137922921No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC11910.10007688526449791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)