FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252360
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG46830.37393401258424097No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35210.2811491903286595No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33370.2664569293174487No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32460.25919064805646935No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30970.24729311060717368No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28010.2236577341978345No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT25880.20664984509246542No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC23360.18652783544667667No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22540.17998019738733273No Hit
GTATATTACTGTGGCAAAGGGAGTAGTACAAGCTGCCATTCTCCCTGCTG20150.16089622792168387No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCACCTATGGCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG19970.15945894151841322No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT18930.15115462007729408No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC18720.149477785940145No Hit
GCGTAATAGTACCAGCAGGGAGAATGGCAGCTTGTACTACTCCCTTTGCC18510.14780095180299593No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17750.14173240921140887No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17340.1384585901817369No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17170.1371011530230924No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT17080.1363825098214571No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16730.13358778625954199No Hit
GCGTAGTACGTGTGACTTCCACTACCACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC16660.13302884154715897No Hit
CCTTTAGCACCTATGGCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG16210.1294356255389824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16180.12919607780510395No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGATCATGCCATAGGTGC15920.12711999744482416No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15890.12688044971094575No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15730.1256028617969274No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC15630.12480436935066594No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15440.12328723370276917No Hit
GTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT15380.1228081382350123No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC15120.1207320578747325No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC15110.12065220863010635No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14850.11857612826982657No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14840.11849627902520042No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC14750.11777763582356511No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14640.1168992941326775No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT14620.11673959564342522No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAGCGT14350.11458366603851929No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT14330.11442396754926698No Hit
GTACTACTCCCTTTGCCACAGTAATATACGGCCGTGTCCTCGGCTCTCAG14170.11314637963524864No Hit
CCTATGGCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGG13640.10891436967006292No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG13280.10603979686352168No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13220.1055607013957648No Hit
GACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTG13060.10428311348174646No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGGTGTTGGAGT13030.10404356574786802No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC12900.10300552556772812No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTAGCACCTATGGCATGATCTGGGTCCG12890.102925676323102No Hit
GCCCAAGGGGGTGGCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAG12850.1026062793445974No Hit
GCCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGG12750.10180778689833594No Hit
CTATTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT12660.10108914369670063No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG12650.10100929445207447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)