FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252360
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47640.3804018013989588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39050.3118113002650995No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA32730.2613465776613753No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32280.2577533616531988No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT29080.2322016033728321No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28510.22765019642914178No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC25140.20074100099013062No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT24650.19682838800344948No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT22680.18109808681209877No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21820.17423105177425022No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCACCTATGGCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG21140.16880130313967232No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC19830.15834105209364718No Hit
GTATATTACTGTGGCAAAGGGAGTAGTACAAGCTGCCATTCTCCCTGCTG19330.1543485898623399No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18180.14516592673033313No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17180.13718100226771854No Hit
GCGTAATAGTACCAGCAGGGAGAATGGCAGCTTGTACTACTCCCTTTGCC16970.13550416813056948No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16030.12799833913571176No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC15950.12735954517870263No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGATCATGCCATAGGTGC15710.1254431633076751No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15520.12392602765977834No Hit
GCGTAGTACGTGTGACTTCCACTACCACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC15460.12344693219202146No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15170.12113130409786323No Hit
CCTTTAGCACCTATGGCATGATCTGGGTCCGCCAGGCTCCAGGGAAGGGG15130.12081190711935866No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT15050.12017311316234948No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC14940.11929477147146189No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT14770.11793733431281739No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14610.11665974639879907No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA14490.11570155546328532No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG14470.11554185697403303No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC14200.11338592736912709No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT14030.1120284902104826No Hit
GTACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT14020.11194864096585647No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG13960.11146954549809959No Hit
GTACTACTCCCTTTGCCACAGTAATATACGGCCGTGTCCTCGGCTCTCAG13830.11043150531795969No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC13670.10915391740394136No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATAGCGT13290.10611964610814782No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA13180.1052413044172602No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA13150.10500175668338178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13090.10452266121562491No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT12980.1036443195247373No Hit
GCCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGG12890.102925676323102No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGATTCACCTTTAGCACCTATGGCATGATCTGGGTCCG12720.10156823916445751No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12580.10045034973969146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)