FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999546
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG45140.4516050286830221No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43120.4313958537175878No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGATCATTACTACTATCC40320.4033831359437185No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGA36320.36336496769533366No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32820.328349070477997No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32490.3250475715975053No Hit
ATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC32490.3250475715975053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC31990.3200453005664572No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29020.29033181064203145No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28630.28643003923781396No Hit
CCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGT27320.27332408913646794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26940.26952236315287137No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT26720.26732136389921024No Hit
GTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCC25480.254915731742211No Hit
CAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGGTTTGCAGTGGTACCAGCAGAGGCCAGGCCAGGCCC25320.2533150050122756No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25170.25181432370296114No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCC24220.24231000874396974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23610.23620723808609106No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT23230.23240551210249455No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22970.22980433116634955No Hit
CTACTATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGAC22950.2296042403251076No Hit
GTGTGGGATAGTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGAC22850.22860378611889798No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22160.2217006520960516No Hit
GGATAGTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG21820.2182991077949389No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21580.2158980177000358No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20900.20909492909781038No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG20700.20709402068539118No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20070.20079115918627058No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20040.20049102292440768No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACAATTAC19950.199590614138819No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19920.1992904778769561No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19780.19788984198826268No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19740.1974896603057788No Hit
GAGTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG19680.19688938778205303No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19400.19408811600466613No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT19380.19388802516342418No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCT19290.19298761637783554No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCTAATTCTGGGAACACGG19040.19048648086231149No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA19020.19028639002106956No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18780.18788529992616648No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18780.18788529992616648No Hit
GGGTGGCCGTGTTCCCAGAATTAGAACCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA18740.18748511824368264No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18570.18578434609312627No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC18470.18478389188691666No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC18470.18478389188691666No Hit
CATTACTACTATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG18300.18308311973636032No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18260.18268293805387648No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA16950.16957698795253046No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG16730.1673759886988693No Hit
GTATCAACGCAGAGTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16710.16717589785762735No Hit
GTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15940.1594724004698133No Hit
GGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTG15840.15847194626360367No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA15680.15687121953366828No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15500.15507040196249097No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15210.15216908476448307No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14380.14386531485294324No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14300.14306495148797554No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14050.1405638159724515No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT14040.1404637705518305No Hit
GTTTGGTCCCTCCGCCGAACACCACACCTCGATGATCATTACTACTATCCCACACCTGGCAGTAATAGTCGGCCT14020.14026367971058862No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13890.1389630892425161No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACAC13870.13876299840127418No Hit
GTACCAGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATA13800.13806268045692746No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13750.13756245335382264No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGC13550.13556154494140338No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG13530.13536145410016148No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGGTTTG13460.13466113615581474No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC13420.1342609544733309No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13310.13316045484650033No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGAT13030.13035918306911337No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12880.12885850175979896No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC12760.1276579567123474No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12530.1253569120380653No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12490.12495673035558144No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12440.12445650325247663No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11950.1195542776420495No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11880.11885395969770277No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11770.11775346007087217No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCTAATTCTGGGAACACGGCCACC11670.11675300586466254No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11620.11625277876155776No Hit
GAAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAAATACA11560.11565250623783198No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC11550.11555246081721102No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11440.11445196119038044No Hit
GGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGCCGT11400.11405177950789659No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11400.11405177950789659No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11260.11265114361920313No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG11210.1121509165160983No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG11120.11125050773050965No Hit
GGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGG11040.11045014436554196No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10940.10944969015933234No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC10670.10674846380256636No Hit
CTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGCCGTCTGTCCTGGGGC10570.10574800959635673No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAA10540.10544787333449386No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10530.10534782791387289No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10400.10404723744580038No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT10360.10364705576331654No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10310.10314682866021173No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10010.10014546604158288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3050.064.2796870
GTCGTAT309.128562E-657.49629639
TCGTATG2450.053.56899640
CGTATGC2500.052.5427941
TCGACGT2950.048.7314629
ACTCGAC2950.047.5354527
AGACTCG2950.047.5045225
ATGCCGT2800.047.06567444
ACGAGAC3050.047.05587822
CTCGACG3000.046.7572428
GACTCGA3000.046.7291526
TCTCGTA2450.046.3937438
CGACGTC3100.046.3897630
GACGTCA3150.045.67860831
CCGTCTT2900.045.63506747
GTGGTAT1950.045.5757181
GGTATCA7750.045.42871
CGAGCCC3200.044.84115615
CTTATAC3350.043.8751341
GTATGCC3100.043.54561242
Produced by FastQC (version 0.11.5)