FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999546
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42310.4232921746472899No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG42070.4208910845523868No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGATCATTACTACTATCC39380.393978866405348No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38190.3820734613514536No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35600.3561616974106244No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGA32350.32364693570881176No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31530.3154432112178929No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTC29580.29593435419680536No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27280.2729239074539841No Hit
GTGTGGGATAGTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGAC26760.2677215455816941No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26130.26141868408257346No Hit
ATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC26000.260118093614501No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25650.2566165038927673No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT25300.25311491417103366No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25060.2507138240761306No Hit
CCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGT24760.2477124614575017No Hit
GGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCC24700.24711218893377596No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24190.2420098724821069No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23950.23960878238720376No Hit
GTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCC23660.23670746518919591No Hit
CAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGGTTTGCAGTGGTACCAGCAGAGGCCAGGCCAGGCCC23390.23400623883242994No Hit
GGGTGGCCGTGTTCCCAGAATTAGAACCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA22940.22950419490448665No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT22920.2293041040632447No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG22810.2282036044364141No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22650.22660287770647874No Hit
GAGTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG20820.20829456573284272No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20340.20349238554303656No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20330.20339234012241558No Hit
CTACTATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGAC20270.20279206759868978No Hit
GCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCT19820.19829002367074655No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACAATTAC19610.19618906983770631No Hit
GGATAGTAGTAATGATCATCGAGGTGTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG19570.19578888815522244No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19420.19428820684590803No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19410.19418816142528708No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19290.19298761637783554No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA18740.18748511824368264No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18500.18508402814877953No Hit
GTATCAACGCAGAGTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT18330.1833832559982232No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18200.18208266553015068No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18130.18138234758580396No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17990.1799817116971105No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG17960.1796815754352476No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC17840.17848103038779606No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17570.1757798040310301No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC17550.17557971318978816No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCTAATTCTGGGAACACGG16970.16977707879377238No Hit
CATTACTACTATCCCACACCTGGCAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG16950.16957698795253046No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA16360.1636743081358937No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT16100.16107312719974867No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15780.1578716737398779No Hit
GTACGGGAGTCCGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15750.15757153747801503No Hit
GGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTG15740.15747149205739405No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA15260.15266931186758786No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15230.152369175605725No Hit
GTGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG15100.1510685851376525No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14920.14926776756647517No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC14430.14436554195604803No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14270.14276481522611265No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC14180.141864406440524No Hit
GTTTGGTCCCTCCGCCGAACACCACACCTCGATGATCATTACTACTATCCCACACCTGGCAGTAATAGTCGGCCT14060.14066386139307246No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGC13790.13796263503630649No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13660.13666204456823397No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13600.1360617720445082No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13330.13336054568774222No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13240.13246013690215358No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13190.13195990979904879No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACAC13140.13145968269594396No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12880.12885850175979896No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12550.1255570028793072No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12500.1250567757762024No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12390.12395627614937181No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC12320.12325595820502509No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12130.12135509521322681No Hit
CTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGCCGTCTGTCCTGGGGC12030.12035464100701718No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCACACCTCGATGAT11970.11975436848329142No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGGTTTG11860.11865386885646083No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11840.1184537780152189No Hit
GGCCTGGCCTCTGCTGGTACCACTGCAAACCTAAACCTCCAACGTTGCTTCCCCCACAGGTAATTGTGGCCGT11820.11825368717397698No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11740.1174533238090093No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG11720.11725323296776737No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11600.11605268792031583No Hit
GTACCAGCAGAGGCCAGGCCAGGCCCCTGTGCTGGTCGTCTATGATGATA11450.11455200661100139No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10960.10964978100057426No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10570.10574800959635673No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC10480.1048476008107681No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGGAAGCAA10420.10424732828704232No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACAC10400.10404723744580038No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10240.102446510715865No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCTAATTCTGGGAACACGGCCACC10230.10234646529524404No Hit
GGACAGACGGCCACAATTACCTGTGGGGGAAGCAACGTTGGAGGTTTAGG10210.10214637445400213No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10190.10194628361276019No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10130.10134601108903443No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT10110.1011459202477925No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10070.10074573856530865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)