FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004691859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55643
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT4080.7332458709990475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.34146253796524273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.3360710242078968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.33067951045055083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.30372194166382116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.24441529033301582No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.22644357780852936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.21386337904138886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.20667469403159427No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.18510863900221053No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.18151429649731324No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.17971712524486458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT930.16713692647772405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.16713692647772405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.15994824146792946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT890.15994824146792946No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1563538989630322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.15096238520568625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT830.14916521395323762No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.141976528943443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.141976528943443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.1365850151860971No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.13478784393364843No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT730.13119350142875114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.12759915892385384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.12759915892385384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.11861330266161063No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT660.11861330266161063No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG640.11501896015671334No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11501896015671334No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10783027514691874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT590.1060331038944701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.1060331038944701No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.10423593264202145No Hit
GTATCAACGCAGAGTACGGGAGGCAGCGGCCCAGTGAAATGCTTCCAACG570.10243876138957282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.10243876138957282No Hit
CTCCAGCCCCCACGTTGCACCAGACCCGTACTCTGCGTTGATAC560.10064159013712416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)