FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420964
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCT109800.7727148611787491No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99960.7034660976632765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC96340.6779904346626656No Hit
ATTTTATACTTCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA80920.5694725552512239No Hit
GCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGAAGTATAAAATACT72310.5088798871751853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68260.48037810950875603No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63410.44624635106871113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58520.41183309358998543No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC58300.41028484887724104No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT57760.40648461185505047No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA57460.40437336906494464No Hit
ATATTATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCAT54810.38572405775234275No Hit
GTTTATTACTGTCACCAATATTATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAA53770.3784050827466424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG52880.37214172913599497No Hit
GCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT50960.35862977527931744No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG50270.35377391686207393No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG49130.3457511942596716No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT48630.34223245627616183No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG45180.3179531641899443No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44300.3117601853389671No Hit
GTATAAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA42280.2975444838855875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC41870.2946591187391095No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT41080.28909951272516404No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC40720.28656602137703696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40200.28290653387418685No Hit
GTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGCCGAAAGTG40120.28234353579682525No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC39750.279739669689028No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATA39040.2747430617524441No Hit
CTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC38530.2711539490092641No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37260.2622163545311493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCC36730.2584864922686289No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA36530.25707899707522497No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACT36170.25454550572709794No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34440.2423706723041541No Hit
ATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGA34100.23997793047536745No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACTTCTCCAA34070.23976680619635682No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33530.23596656917416625No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT32140.22618447758000906No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG31560.2221027415191377No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC30820.21689500930354325No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30520.21478376651343734No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29100.20479055064026955No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28830.2028904321291743No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28550.20091993885840878No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACTCCCTTCACTTT27430.19303796577534688No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT27110.19078597346590062No Hit
CGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27100.1907155987062304No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA27080.19057484918689002No Hit
GTCAGGGACCCCGGATTCCCGGTTAGAGGCCCAGGTAATGAGCAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTA26990.18994147634985825No Hit
CCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG26580.18705611120338023No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26470.18628198884700808No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26270.18487449365360417No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT26030.18318549942151946No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT25400.17875188956229715No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC25100.17664064677219127No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25060.1763591477335105No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24780.174388654462745No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24750.17417753018373444No Hit
CTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATC24710.17389603114505364No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT24690.17375528162571324No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24410.17178478835494776No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA24060.16932167176649093No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG23930.1684067998907784No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23880.1680549260924274No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23640.16636593186034268No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC23490.16531031046528977No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACTTCTCCAACAATAAGAACTA23320.16411393955089643No Hit
GGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC23230.16348056671386466No Hit
GAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT23090.16249532007848191No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22550.15869508305629135No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACTCCCT22540.15862470829662118No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22530.15855433353695098No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGC22350.15728758786288746No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG22150.15588009266948355No Hit
CTTTGGTCCCAGGGCCGAAAGTGAAGGGAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGC22140.15580971790981332No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT22030.15503559555344118No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21830.15362810036003727No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG21770.1532058518020161No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21520.15144648281026118No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21200.14919449050081493No Hit
ACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATT20920.14722399723004947No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20600.1449720049206032No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19980.14060876982105106No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC19940.14032727078237028No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19910.14011614650335968No Hit
GGTAATGAGCAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTA19650.1382864027519346No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19650.1382864027519346No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA19380.13638628424083932No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGG19280.13568253664413737No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19180.1349787890474354No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19150.1347676647684248No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT19010.1337824181330421No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG18850.13265642197831895No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18780.13216379866062758No Hit
TTATTACTGTCACCAATATTATCGTACTCCCTTCACTTTCGGCCCTGGGA18440.12977105683184093No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA18420.12963030731250053No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCT18300.12878581019645818No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18260.12850431115777738No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG18140.12765981404173504No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAA18010.1267449421660225No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC17910.12604119456932056No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17790.1251966974532782No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17740.12484482365492722No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17330.12195945850844919No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG17220.12118533615207705No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC17120.1204815885553751No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17120.1204815885553751No Hit
GTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCTGA16680.11738509912988648No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT16630.11703322533153548No Hit
CTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC16530.11632947773483353No Hit
GCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTC16500.11611835345582296No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16430.11562573013813157No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16410.11548498061879119No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC16040.11288111451099395No Hit
AACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAA15930.11210699215462179No Hit
GCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGCCGAAAGTGAAGGG15920.1120366173949516No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15680.1103476231628669No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG15600.10978462508550532No Hit
GTATTTTATACTTCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTG15570.10957350080649474No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT15560.10950312604682455No Hit
CTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCA14970.10535101522628301No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG14870.10464726762958104No Hit
ACCTGGGCCTCTAACCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAG14800.10415464431188969No Hit
GTGAAGGGAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTC14780.10401389479254927No Hit
GCTAAGTAGTTCTTATTGTTGGAGAAGTATAAAATACTCTGGCTGGACTT14740.1037323957538685No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG14740.1037323957538685No Hit
CACCAATATTATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGG14710.10352127147485791No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGC14680.10331014719584732No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14600.10274714911848576No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT14530.10225452580079439No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGTT14460.10176190248310302No Hit
GACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC14310.10070628108805008No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14270.10042478204936929No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACTTCTCCAACAATAA14210.10000253349134813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)