FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1420964
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97520.6862946563037488No Hit
CTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCT94870.6676453449911469No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC78140.5499083720629094No Hit
GCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGAAGTATAAAATACT70160.4937493138460932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC69800.49121582249796614No Hit
ATTTTATACTTCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA68960.4853043426856697No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67810.47721124532359727No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT58270.4100737245982305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57110.4019102524764878No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54830.38586480727168315No Hit
GTTTATTACTGTCACCAATATTATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAA53760.3783347079869722No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52800.3715787310586334No Hit
ATATTATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCAT52630.37038236014424014No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA51620.36327450941755035No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA49070.3453289457016504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47040.33104286948860073No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT46420.32667963438904857No Hit
GCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT45810.3223867740491666No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45520.320345906018731No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG45090.3173197913529125No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43700.30753769975875533No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43530.306341328844362No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40540.2852992757029735No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40270.2833991571918782No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40050.2818509124791339No Hit
GTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGCCGAAAGTG39850.28044341728572997No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT38680.2722095704043171No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA38440.27052057617223235No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC37980.26728333722740333No Hit
GTATAAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA37730.2655239682356485No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC35710.2513082667822689No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACT35320.2485636511551313No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCC35320.2485636511551313No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATA33720.2373036896079No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33060.23265895546966706No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGTT32720.2302662136408804No Hit
ATCGTACTCCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGA31730.22329911243353104No Hit
CTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC31570.22217311627880793No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACTTCTCCAA30550.21499489079244796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30050.21147615280893814No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG29730.2092241604994919No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29530.207816665306088No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG29440.2071832924690562No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA28630.20148293693577038No Hit
CGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC28320.1993013193859943No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28020.19719007659588844No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27470.19331946481402765No Hit
GAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT27090.19064522394656022No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT26970.18980072683051788No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC26400.1857893655293167No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25900.18227062754580692No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25720.1810038818717434No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25640.18044088379438183No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA25110.17671102153186147No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25080.17649989725285087No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC25040.1762183982141701No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24820.17467015350142578No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACTCCCTTCACTTT24700.17382565638538344No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCACCAATATTATCGTACTCCCT24550.1727700349903305No Hit
CCCTTCACTTTCGGCCCTGGGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG24480.17227741167263913No Hit
GTCAGGGACCCCGGATTCCCGGTTAGAGGCCCAGGTAATGAGCAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTA24410.17178478835494776No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24190.17023654364220345No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC24060.16932167176649093No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24030.16911054748748033No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC23940.16847717465044856No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23770.16728080373605525No Hit
CTTTGGTCCCAGGGCCGAAAGTGAAGGGAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTCAGC23070.16235457055914154No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG22970.1616508229624396No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22710.1598210792110145No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA22570.15883583257563175No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22120.15566896839047295No Hit
GGACCAAAGTGGGTATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC21890.15405034891805844No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21840.15369847511970747No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG21770.1532058518020161No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21530.1515168575699314No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20780.14623875059466673No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20750.14602762631565613No Hit
CTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATC20520.14440900684324162No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20330.1430718864095079No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20280.14272001261115694No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20170.1419458902547848No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC20150.1418051407354444No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19920.14018652126302988No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19760.13906052510830677No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19760.13906052510830677No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19720.13877902606962597No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACTTCTCCAACAATAAGAACTA19710.13870865130995577No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19640.1382160279922644No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19000.1337120433733719No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18960.1334305443346911No Hit
TTATTACTGTCACCAATATTATCGTACTCCCTTCACTTTCGGCCCTGGGA18500.1301933053898621No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGC18430.12970068207217073No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18400.12948955779316013No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTCCGGGGACATCGTGATGA18170.12787093832074561No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT17990.1266041926466821No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT17710.12463369937591663No Hit
GGTAATGAGCAGCTTAGGAGGCTGTCGTGGTTTCTGCTGGTACCAAGCTA17600.12385957701954449No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGG17560.1235780779808637No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG17400.12245208182614056No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG17390.12238170706647038No Hit
ACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATT17270.12153720995042802No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT17010.11970746619900292No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16890.11886296908296058No Hit
CTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG16860.11865184480395No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16710.11759622340889707No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16580.11668135153318451No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16500.11611835345582296No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16270.11449973398340846No Hit
GTGAAGGGAGTACGATAATATTGGTGACAGTAATAAACTGCCACATCTTC16220.11414786018505746No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16200.11400711066571709No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG16030.11281073975132376No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15720.11062912220154769No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT15500.10908087748880337No Hit
TCCGGGGACATCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCT15470.10886975320979278No Hit
GCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGCCGAAAGTGAAGGG15280.10753263277605907No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15090.10619551234232534No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT15050.10591401330364457No Hit
CAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAA15030.10577326378430418No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT14980.1054213899859532No Hit
GTCTGGAGACTGGGTCATCACGATGTCCCCGGAGGCACCAGAGATCCAGAGCAACAGAGAAATGAAGACCTGGGT14970.10535101522628301No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATACCCACTTTGGTCCCAGGGC14890.10478801714892143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14320.10077665584772029No Hit
GTACCAGCAGAAACCACGACAGCCTCCTAAGCTGCTCATTACCTGGGCCTCTAACCGGGAATCCGGGGTCCCTGA14230.10014328301068852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.043.2641941
TGGTATC4700.035.163492
TATCAAC35900.032.600842
GATTCAC3000.031.4963341
ATCAACG37150.031.4042723
TCAACGC37400.031.1030964
CAACGCA37750.030.906225
AACGCAG38350.030.5095316
CGCAGAG40100.029.1780688
AGAGTAC40400.028.9583411
TGTTAGA28300.028.52960870
ACGCAGA41000.028.4553327
GCAGAGT41750.028.0239379
GGTCTGG44800.027.64399370
GTCTTAC1006.0878665E-726.9968591
ATTCACT3700.025.5240762
AGTCGTC750.001364222324.54810165
AATGGTA4200.024.4366870
GTGCAAC700.00151722724.1043361
GCCTGAC8400.024.084827
Produced by FastQC (version 0.11.5)