FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280619
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCCTTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC184010.8068423528875275No Hit
CCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC153720.6740275337528978No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC94990.41650972827990995No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAAAGGCCACTACCCATATACAGCACACAGTAATAAT88220.38682480502004063No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAAAGGCCACTACCCATATACAGCACA84950.3724865924558201No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGG84850.372048115007373No Hit
GATTATTACTGTGTGCTGTATATGGGTAGTGGCCTTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT83040.3641116731904803No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG81850.3588937915539597No Hit
CTCATCTACAGCACAAACATCCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG78310.34337168987893196No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG76620.3359614210001758No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG75230.329866584466761No Hit
CTCTACTAGTGATCACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA65720.2881673791194408No Hit
CTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGG63160.2769423564391948No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC62490.2740045575345992No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT62270.2730399071480155No Hit
GTCTCTACTAGTGATCACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC61250.268567437173855No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG59440.26063099535696227No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT58400.25607082989311236No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG55940.24528428466131344No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT55020.2412502921356No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC54070.23708475637535248No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG53690.23541854207125346No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT53120.23291922061510492No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC53060.23265613414603664No Hit
CTACTAGTGATCACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCA52040.22818366417187616No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG51480.2257281904605723No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50570.22173804567970362No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT50380.22090493852765408No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG50010.2192825719683998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG49330.21630092531895947No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC48740.21371390837312151No Hit
CCCCAGAAGAGCGGATGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG47970.21033763202007874No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACATCCGCTCT46710.20481281616964517No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG45210.1982356544429385No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAT44770.19630635366977126No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA44470.1949909213244299No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG43310.18990458292244342No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42620.18687908852815838No Hit
ACCCATATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT41750.1830643347266685No Hit
AGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTC41520.18205583659524016No Hit
CCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCT41040.17995114484269403No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTGATCACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC41020.1798634493530046No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC40450.17736412789685607No Hit
GTGCTGTATATGGGTAGTGGCCTTTGGATGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGG39140.17162007332219895No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT39000.171006204894373No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38970.17087466165983883No Hit
CATCTACAGCACAAACATCCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC38940.1707431184253047No Hit
ATACAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA38540.16898920863151626No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC37780.16565678002331824No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA37540.16460443414704518No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACAGTCACA37030.1623681991599649No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAAAGGCCACTAC35970.15772033820642553No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC35500.1556594941987241No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35120.1539932798946251No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34340.15057315579673763No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT34330.1505293080518929No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC34100.14952080992046457No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTGATCACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG33400.1464514677813348No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG33190.1455306651395959No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT33130.14526757867052761No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT33120.14522373092568291No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTGATCACTAGTAGAGACTGAG33120.14522373092568291No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA32610.14298749593860263No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32130.1408828041860565No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31690.1389535034128892No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC31580.1384711782195974No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31550.13833963498506327No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAG31440.13785730979177144No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAAATACA31370.13755037557785846No Hit
CACTAGTAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTGTGACTGTC30870.13535798833562293No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC30800.13505105412170995No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30740.13478796765264167No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG30660.13443718569388396No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC30200.1324201894310273No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29820.13075397512692827No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC29230.1281669581810903No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA29060.12742154651873022No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGC28680.1257553322146312No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC28670.1257114844697865No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC28000.12277368556519086No Hit
CCTTGGTCCCTCCGCCGAACATCCAAAGGCCACTACCCATATACAGCACACAGTAATAATCAGATTCATCATCTG27820.12198442615798605No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT27690.12141440547500482No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA27010.11843275882556448No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC26870.1178188903977385No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATC26760.11733656520444669No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTACTAGTGATCA26600.1166350012869313No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC25530.11194329258854722No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACATCCAAAGGCCA25270.11080325122258473No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCGTT24620.10795314780767853No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG24420.10707619291078431No Hit
AGCCACAAGTGAGTGTGACTGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTCTGAGAAT24340.10672541095202662No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC24300.10655001997264778No Hit
TTATTACTGTGTGCTGTATATGGGTAGTGGCCTTTGGATGTTCGGCGGAG24190.10606769477935596No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC23960.1050591966479276No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTGTATATGGGTA23890.10475226243401461No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23780.10426993724072281No Hit
GGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG23640.10365606881289685No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT23400.10260372293662379No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA23390.10255987519177907No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC23280.10207754999848725No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23220.10181446352941897No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC23140.1014636815706613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)