FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43710.5292696659825272No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40740.49330693644768153No Hit
CAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCAT36370.44039207851257184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29810.36095924829419207No Hit
GTGTAACTGTGTTGACAATAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAGCTGTC28800.3487294985197159No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28720.34776080546827226No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26770.3241489123393332No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26650.3226958727621677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25420.3078022170962215No Hit
GTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT24430.29581464058460627No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCACCGATTTAAATTGGTATCAGCAGAAA24390.29533029405888445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24020.2908500886959575No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23760.28770183627876567No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22380.2709918811413626No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22350.2706286212470712No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22300.27002318808991893No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAAGT22120.2678436287241707No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21920.26542189609556155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21490.26021517094405194No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20350.24641129496097985No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20270.24544260190953615No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCAC20080.2431419559123575No Hit
GTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCCCCTGGCCAAAACGG18750.22703743393210674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18580.22497896119778896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18530.22437352804063668No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18250.22098310236058388No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18190.22025658257200115No Hit
GCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAACACAGTTACACTTCC17790.21541311731478285No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17740.21480768415763057No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17560.21262812479188234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCC16340.1978555557573666No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15850.1919223108172742No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15320.18550471935146No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14460.17509126904844072No Hit
GAGTGAAAGCTGTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA14400.17436474925985795No Hit
GTCTGGGACCTGAGGACACGGCCTTCTATTACTGTGTCAAAGATCAGAGT14250.17254844978840111No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13970.16915802410834832No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13770.16673629147973917No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13000.15741262085959398No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12930.15656501443958082No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12810.15511197486241532No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12770.15462762833669347No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATCGGTGATA12730.15414328181097167No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12400.15014742297376657No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12380.14990524971090566No Hit
GTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAA12190.14760460371372697No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11810.1430033117193696No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11710.14179244540506505No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11710.14179244540506505No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11560.1399761459336082No Hit
TCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC11510.13937071277645594No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11280.13658572025355542No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11150.13501159404495947No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG11090.13428507425637673No Hit
GCCCCTAACCTCCTGATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGT11050.1338007277306549No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11020.1334374678363635No Hit
AGTCAGACCATTATCACCGATTTAAATTGGTATCAGCAGAAACCAGGGAA10990.13307420794207214No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10950.13258986141635032No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10820.1310157352077544No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10650.1289572624734366No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10650.1289572624734366No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10490.12701987637054932No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10480.12689878973911886No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAAC10380.12568792342481427No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10260.1242348838476488No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10240.12399271058478789No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10220.12375053732192698No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10110.12241858437619195No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10080.12205532448190057No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10030.12144989132474829No Hit
ACCTTGATGGGACCCCAGTTTGCAAAGTGACTGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG10000.12108663143045692No Hit
CCCCTAACCTCCTGATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCA9970.12072337153616555No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT9910.1199968517475828No Hit
TGTCAACACAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCA9850.11927033195900008No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCA9830.11902815869613914No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9830.11902815869613914No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9790.11854381217041732No Hit
GGCCAAAACGGTAGGGGGAAGTGTAACTGTGTTGACAATAGTAAGTTGCA9750.11805946564469551No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9740.11793837901326505No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9590.11612207954180818No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9480.11479012659607316No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA9470.1146690399646427No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9460.11454795333321224No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA9370.11345817365033813No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9290.11248948059889448No Hit
CATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTG9280.11236839396746402No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9220.11164187417888127No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9190.1112786142845899No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9070.10982557470742443No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9030.10934122818170261No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9020.10922014155027213No Hit
GTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAACACAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGA8850.10716166881595438No Hit
CTGCTGATGGTGAGAGTGAAAGCTGTCCCAGACCCACTGCCACTGAACCT8820.10679840892166301No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8790.10643514902737164No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8700.10534536934449752No Hit
CAGTAATAGAAGGCCGTGTCCTCAGGTCCCAGACTGTCCATTTGCAGAAA8690.10522428271306707No Hit
CCATTATCACCGATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCAGTCA8600.10413450303019295No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG8590.10401341639876249No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8590.10401341639876249No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8580.10389232976733204No Hit
GTAGGGGGAAGTGTAACTGTGTTGACAATAGTAAGTTGCAGAATCTTCAG8480.10268146345302748No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8380.1014705971387229No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCATCAAGGTT8340.10098625061300108No Hit
CACCATCACTTGCCGGGCAAGTCAGACCATTATCACCGATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCC8330.10086516398157062No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8330.10086516398157062No Hit
CCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAACAC8330.10086516398157062No Hit
GATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGCTTTCA8270.10013864419298787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC750.055.21970441
ACCGTAC750.054.6505327
GCGACTT200.00662650153.13902358
TCGTATG800.051.7146140
ATCGGGC200.007699294551.1604238
GACCGTA850.048.20644426
CGTACTA900.045.5669729
ACGAGAC900.045.49521622
CTTATAC1050.042.2481161
GTATCAA13700.039.8522381
GTGGTAT1200.039.8107261
ATCTCGT955.456968E-1239.78613337
CTAGATC955.456968E-1239.66516533
ATGCCGT1050.039.57842644
TTAACGG350.001385815639.20486535
GTACTAG1050.039.07874330
CCCACGA1050.038.9817319
ACTAGAT1100.037.35004832
TCTCCGA1100.037.2120911
ATCTCCG1100.037.20983510
Produced by FastQC (version 0.11.5)