FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43560.5274533665110703No Hit
CAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCAT32960.399101537194786No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32370.391957425940389No Hit
GTGTAACTGTGTTGACAATAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAGCTGTC29500.35720556271984794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28760.3482451519939941No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28620.3465499391539677No Hit
GTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT25610.3101028630934002No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25560.30949742993624785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24310.2943616010074408No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23210.28104207155009053No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22890.2771672993443159No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCACCGATTTAAATTGGTATCAGCAGAAA22510.2725660073499585No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCAGTTTGCAAAGT21780.26372668325553517No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTATCAC21190.2565825720011382No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20630.24980172064103262No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20200.244594995489523No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19740.23902501044372199No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19530.23648219118368236No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19300.23369719866078187No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19210.23260741897790774No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19100.23127546603217272No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC19070.23091220613788133No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18990.22994351308643768No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18830.22800612698355036No Hit
GTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCCCCTGGCCAAAACGG18750.22703743393210674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18360.2223150553063189No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18060.21868245636340522No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17290.20935878574326003No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17090.20693705311465088No Hit
GCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAACACAGTTACACTTCC16830.203788800697459No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16250.19676577607449247No Hit
GTCTGGGACCTGAGGACACGGCCTTCTATTACTGTGTCAAAGATCAGAGT16030.19410187018302244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16020.19398078355159198No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGGATCTCCAGCTTGGTCC15370.1861101525086123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15260.18477819956287725No Hit
GAGTGAAAGCTGTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCA13780.16685737811116963No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13640.16516216527114325No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13140.1591078336996204No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA13120.15886566043675948No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13030.15777588075388538No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12930.15656501443958082No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12870.15583849465099806No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATCGGTGATA12730.15414328181097167No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12290.14881547002803155No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12050.14590939087370058No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11990.14518287108511785No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11880.14385091813938283No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11780.14264005182507825No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11710.14179244540506505No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11570.14009723256503864No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11440.13852310635644272No Hit
GTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAA11180.13537485393925083No Hit
AGTCAGACCATTATCACCGATTTAAATTGGTATCAGCAGAAACCAGGGAA11100.13440616088780719No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11100.13440616088780719No Hit
GCCCCTAACCTCCTGATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGT10800.13077356194489348No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10800.13077356194489348No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10780.13053138868203254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10720.12980486889344983No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAAC10440.12641444321339704No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10380.12568792342481427No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10380.12568792342481427No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT10340.12520357689909245No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10220.12375053732192698No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG10100.1222974977447615No Hit
TCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC10040.12157097795617874No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10000.12108663143045692No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9840.1191492453275696No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9730.1178172923818346No Hit
GTCTGCAACCTGAAGATTCTGCAACTTACTATTGTCAACACAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGA9570.11587990627894727No Hit
ACCTTGATGGGACCCCAGTTTGCAAAGTGACTGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTG9550.11563773301608636No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9510.11515338649036454No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9240.11188404744174218No Hit
CCCCTAACCTCCTGATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCA9210.11152078754745082No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9150.1107942677588681No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA9140.11067318112743764No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9100.11018883460171579No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA9080.10994666133885489No Hit
GGCCAAAACGGTAGGGGGAAGTGTAACTGTGTTGACAATAGTAAGTTGCA9070.10982557470742443No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9070.10982557470742443No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9050.10958340144456352No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9030.10934122818170261No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT9020.10922014155027213No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9010.10909905491884167No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8990.10885688165598077No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCACCA8970.10861470839311986No Hit
TGTCAACACAGTTACACTTCCCCCTACCGTTTTGGCCAGGGGACCAAGCTGGAGATCCAACGAACTGTGGCTGCA8900.10776710197310665No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8850.10716166881595438No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8770.10619297576451071No Hit
GATCTATGATGCAGTCACTTTGCAAACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGCTTTCA8710.10546645597592798No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8710.10546645597592798No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8620.10437667629305386No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8500.10292363671588838No Hit
CATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGCTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTG8490.10280255008445792No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8480.10268146345302748No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCA8410.10183385703301426No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8270.10013864419298787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC200.007747655351.0794515
GTGGTAT2000.049.367821
CGCACTT6050.040.09558570
TCGTCAC400.002773097534.03851710
GTATCAA15250.033.7118871
AATGGTA3300.031.78748970
AGTTTAT2100.030.8060872
TATCAAC16800.030.6034162
AACTAGC450.004732247430.51912536
GCTTGTA450.004930762630.2637961
ATCAACG17150.029.7839323
TCAACGC17150.029.7767124
GACAGGT1501.8189894E-1229.5072021
GAGTTTA2200.029.4040321
CAACGCA17350.029.2372425
AACGCAG17500.028.9866376
ACGGCCT4800.027.66971218
GTGACGA500.00808449527.35016631
AGTGACG500.00810853627.33352930
ACGCAGA18650.027.1992577
Produced by FastQC (version 0.11.5)