FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339722
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39440.29438943303162896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31850.23773588849029872No Hit
GTGTATGCATCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTCTTTGCTGATACCAGGCTAAATGACTACTCATG29380.21929922775023475No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28870.215492467840343No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27630.20623681629472385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26320.19645866829088424No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23630.1763798758249846No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23230.17339418177801066No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23090.1723491888615698No Hit
TGCATACACCTTGCAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACAAT22380.16704958192819108No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21980.16406388788121715No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20560.1534646740144597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20080.149881841158091No Hit
GGGCATGAGTAGTCATTTAGCCTGGTATCAGCAAAGACCAGGGAAAGCCCCTAAGCTCCTGATCTATGATGCATA19580.14614972359937362No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19580.14614972359937362No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18630.13905870023781053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18150.13547586738144182No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17590.13129589571567832No Hit
GTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA17440.1301762604480631No Hit
GAGTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTGATAGTTAT17310.1292059098827966No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16810.12547379232407918No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16800.12539914997290483No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16540.12345844884237178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16320.12181631711653612No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15810.11800955720664436No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15430.11517314786201913No Hit
GCTTGATAGTTATCCTTTATGCAGTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT15340.11450136670145002No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAACTG14710.10979889857746607No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATGAGTAGTCATTTAGCCTGGTATCAGCAAAGA14400.1074849856910613No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14030.1047232186976104No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13980.10435000694173865No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13970.10427536459056432No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC13930.10397679518586692No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13810.10308108697177473No Hit
CTATCAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTC13780.1028571599182517No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA13510.1008418164365443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)