FastQCFastQC Report
Wed 8 May 2024
EGAF00004691881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968614
Total Bases72 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT57250.5910507178298062No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC54720.5649309219152314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53410.5514064426076848No Hit
GTATAATAACTGGCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC47310.4884298595725438No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA39390.4066635419269183No Hit
CATCTATGCTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTG37830.3905580551179314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36920.38116318781268904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36620.3780659788109608No Hit
GTCTCCAGCCATCCTGCCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCT35850.37011647570652495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34190.352978585896962No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT33390.34471936189235336No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT33390.34471936189235336No Hit
GGCCAAAAGTGTGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAA31560.3258263869818111No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGC30930.3193222480781818No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGCGGCCCTGGT30840.3183930853776633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29220.3016681567683308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG28770.2970223432657385No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC28720.2965061417654504No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27860.28762747596049615No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA27820.2872145147602657No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27620.28514970875911355No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA27400.2828784221578462No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27190.2807103758566364No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC27150.28029741465640595No Hit
GCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC26320.27172846975162446No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT26130.26976690405052994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24440.25231929334079417No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCATCCTGCCTGTGTCTCCAGG23390.24147906183474532No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT22660.23394251993053994No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC22520.2324971557297334No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC21600.22299904812443347No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21520.22217312572397258No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA21010.2169078704210346No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCACACTTTTGGCCAGGGGAC20910.21587546742045852No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20910.21587546742045852No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGA19920.20565467771475532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19820.20462227471417924No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA19320.19946025971129883No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG19310.1993570194112412No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC19300.1992537791111836No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC19080.19698249250991623No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC19030.1964662910096282No Hit
CACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGA18970.19584684920928253No Hit
GTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCC18970.19584684920928253No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT18570.1917172372069782No Hit
GAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTC18510.19109779540663258No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18020.1860390207038098No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT17690.1826320908019087No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCATCCACCA16710.1725145413962631No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15880.16394559649148163No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15580.16084838748975339No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACT15530.16033218598946536No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15510.16012570538935014No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCC15470.1597127441891197No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15240.15733821728779473No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15170.15661553518739146No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14880.15362156648572084No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14600.1507308380841078No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG14580.1505243574839926No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAGCAACTTAGCCTGGTA14160.14618826488157305No Hit
GTCAGAGTGTTAACAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAG13980.1443299394805361No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGT13730.1417489319790959No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13720.1416456916790383No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13680.14123273047880786No Hit
CTCCCAGGCTCCTCATCTATGCTGCATCCACCAGGGCCGCTGGTATCCCA13550.13989060657805896No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCC13540.13978736627800137No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCT13520.13958088567788612No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCATCCTGC13520.13958088567788612No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13310.13741283937667637No Hit
ATAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGAAACA13130.13555451397563942No Hit
ATGCTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGT13110.1353480333755242No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12990.1341091497748329No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTAACACTCTGACTGGCCCT12940.13359294827454488No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12910.13328322737437204No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12900.13317998707431444No Hit
GCCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT12810.13225082437379596No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAG12790.13204434377368074No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12730.1314249019733351No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12720.1313216616732775No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12710.13121842137321987No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC12710.13121842137321987No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA12630.130392498972759No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA12460.12863741387177968No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT12430.12832769297160684No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCC12320.12719204967097317No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12310.12708880937091557No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT12190.12584992577022425No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12160.12554020487005144No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA12160.12554020487005144No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12150.12543696456999384No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12100.1249207630697058No Hit
CTGCAGGAGAGGGTGGCTCCTTCCCCTGGAGACACAGGCAGGATGGCTGG12090.12481752276964816No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12070.12461104216953296No Hit
TCTATGCTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGC12040.12430132126936012No Hit
CAATAATACACAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGCAGAAA11940.12326891826878404No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGTTA11780.12161707346786232No Hit
GCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTC11640.12017170926705582No Hit
CTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAAC11640.12017170926705582No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11460.11831338386601886No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11440.11810690326590366No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11400.11769394206567323No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11350.11717774056538519No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11340.11707450026532758No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCATCCTGCCTGTGTCT11070.11428701216377214No Hit
GGATACCAGCGGCCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCC10850.11201572556250478No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10720.11067360166175588No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGAT10710.11057036136169825No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10710.11057036136169825No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10700.11046712106164065No Hit
GTATCAACGCAGAGTACGGGGAGGAACTGCTCAGTTAGGACCCAGACGGA10600.10943471806106457No Hit
GTGTATTATTGTGCGAGAGAACGAAGCTGGGCTTATATTGACTACTGGGG10560.10902175686083415No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10540.10881527626071892No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10520.10860879566060372No Hit
CTCCAGCCATCCTGCCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGC10510.10850555536054611No Hit
GATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGT10480.10819583446037327No Hit
TTATTACTGTCAGCAGTATAATAACTGGCACACTTTTGGCCAGGGGACCA10350.10685371055962437No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10240.10571806725899068No Hit
GTATTATTGTGCGAGAGAACGAAGCTGGGCTTATATTGACTACTGGGGCC9930.10251761795720482No Hit
CTCCTCATCTATGCTGCATCCACCAGGGCCGCTGGTATCCCAGCCAGGTT9900.10220789705703201No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9760.1007625328562255No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT9740.10055605225611028No Hit
ATATAAGCCCAGCTTCGTTCTCTCGCACAATAATACACAGCCGTGTCCTC9730.10045281195605266No Hit
GTTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCCTTCCCCTGGAG9690.10003985075582225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)