FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543569
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC63620.41216168502995326No Hit
GCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTG60710.3933092722126449No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG53400.34595149293617583No Hit
GGTTAACAGTGATGGCAGTCAATACAAGGGGGGCGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTCA51650.3346141312762824No Hit
TCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46300.29995419705889403No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT45440.29438269361460356No Hit
ATACAAGGGGGGCGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTCACCGCTTCCTCACCATCTCCAG45060.29192086651131244No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43560.2822031279456895No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38770.25117114945946695No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38300.24812625804223848No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG38110.24689534449059292No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35850.2322539517183877No Hit
CTGTTAACCTGCATCAAGAACCGAGGGCCCTTCTCTGGCTGCTGCTGATGCCATGCGATGGCGTAGGTGCTGTGG34400.2228601377716189No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT33680.21819562326011988No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32830.21268890473960023No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31910.20672869175268485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31780.20588648774366422No Hit
GTCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31610.20478514403956027No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31000.2008332636895403No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT30920.20031498429937375No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACTCAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAG30010.19441955623622917No Hit
GTAGTATGCGGCCGTGTCGTCGGCTCTCAGGCTGCTCATTTGCAGATACA29250.1894959020296469No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGC28840.18683972015504327No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC28780.18645101061241837No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28010.18146257148206527No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27920.1808795071681279No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27880.1806203674730446No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC27790.18003730315910724No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27700.17945423884516987No Hit
CCTGCACTCTGAGCAGTGGCCACAGCACCTACGCCATCGCATGGCATCAG27130.17576149819023315No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27040.17517843387629578No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC26580.17219832738283808No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25310.16397064206394402No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT25030.16215666419836106No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGG24750.16034268633277812No Hit
CCCTTGTATTGACTGCCATCACTGTTAACCTGCATCAAGAACCGAGGGCC24480.158593493390966No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24230.15697387029669552No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23440.1518558613188008No Hit
CACCTGCACTCTGAGCAGTGGCCACAGCACCTACGCCATCGCATGGCATC23220.15043059299584277No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22930.148551830206489No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC22850.14803355081632244No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC22490.14570129356057293No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGCGGTGAGCCCCAGAGCTGGAGC21320.13812145747938706No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21270.13779753286053295No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20900.13540049068101265No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20830.1349469962146169No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT20770.134558286671992No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20600.13345694296788807No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACTCAGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCC20600.13345694296788807No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20490.13274430880640906No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20320.1316429651023051No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20300.13151339525476347No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG20250.13118947063590936No Hit
TCCTCAGACTGGAGGCTGGAGATGGTGAGGAAGCGGTGAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC20080.13008812693180544No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19620.12710802043834776No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19600.1269784505908061No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19460.12607146165801464No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19450.12600667673424382No Hit
GCTCTGGGGCTCACCGCTTCCTCACCATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC19300.12503490287768154No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18830.12199001146045302No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18780.12166608684159891No Hit
CCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCA18100.11726071202518319No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACTC17920.11609458339730844No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT17800.11531716431205863No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17780.11518759446451697No Hit
CCTTGGTCCCTCCGCCGAACACCTGAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA17340.11233705781860091No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16660.1079316830021852No Hit
GCATACTACTGTGCGAAACTCGCGCATCCCTCTTGTGCTGGTGGTAGTCG16590.10747818853578946No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16580.10741340361201863No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACAC16320.10572899559397735No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16090.10423894234724848No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15750.10203625493904063No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15440.10002792230214522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)